Method for evaluating maximum elongation characteristics of mesocotyl and coleoptile of Zea mays

A technology of elongation characteristics and coleoptiles is applied in the field of screening maize germplasm resources resistant to deep sowing and evaluating the maximum elongation characteristics of maize mesocotyls and coleoptiles. and efficiency, deep sowing resistance, time-consuming and labor-intensive, etc.

Active Publication Date: 2021-06-22
GANSU AGRI UNIV +1
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Problems solved by technology

However, using these indicators to evaluate the deep-sowing tolerance of maize germplasm resources is time-consuming and laborious, and the operation is difficult, which is not conducive to the rapid screening of high-quality materials in large quantities, and seriously restricts the progress and efficiency of maize deep-sowing tolerance breeding.
Whether the physical properties such as size, mass, specific gravity and bulk density of maize seeds regulated by genetic characteristics and the nutritional qualities such as starch, protein, fat and various amino acids have stable direct and direct effects on the elongation characteristics of maize mesocotyls and coleoptiles The indirect effect, the extent of its correlation, and whether the physical characteristics and nutritional quality of corn seeds can be used to reflect the deep-sowing resistance of corn need to be further studied

Method used

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  • Method for evaluating maximum elongation characteristics of mesocotyl and coleoptile of Zea mays
  • Method for evaluating maximum elongation characteristics of mesocotyl and coleoptile of Zea mays
  • Method for evaluating maximum elongation characteristics of mesocotyl and coleoptile of Zea mays

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Embodiment 1

[0060] The invention provides a method for screening evaluation parameters of the maximum elongation characteristics of corn mesocotyls and coleoptiles, and the specific screening method is carried out according to the following steps:

[0061] 1. Seed selection: select 2 strong deep-sowing inbred lines N192 (German material), W64A (O 2 o 2 BC 3 ) and 2 seeds of intolerant deep-sowing inbred lines Ji853 ([Huangzao 4 / Zi330] / Huangzao 4 offspring) and K12 (Huangzao 4 / Weichun offspring) seeds.

[0062] 2. Field planting of corn materials: Seeds of 4 corn inbred lines including N192, W64A, Ji853 and K12 were sown in Zhangye Experimental Field of Gansu Academy of Agricultural Sciences in mid-April 2019 (http: / / zyc.gsagr.ac.cn / channels / channel_486_1.html; 38.93°N, 100.43°E, altitude 1570m, annual precipitation 129mm). Seeds of corn inbred lines were planted according to a completely random block design, and each inbred line had 7.5m of material 2 , two-row area, row spacing 0.3...

Embodiment 2

[0068] The present invention provides a screening result of evaluation parameters for the maximum elongation characteristics of corn mesocotyls and coleoptiles, and the specific screening results are as follows:

[0069] 1. Analysis of the physical characteristics of the seeds of different genotypes of corn inbred lines: there were large differences in the appearance and morphology of the seeds of the 4 corn inbred lines with large differences in deep sowing resistance (P figure 2 And attached image 3 ). Among them, the maximum grain length, grain width, and seed shape coefficient of the deep-seeding inbred line N192 were 12.3 mm, 10.4 mm, and 11.1, respectively, while the maximum grain thickness of the non-deep-seeding inbred line K12 was 5.0 mm (attached figure 2 And attached image 3 ). It indicated that the difference in appearance and morphology of maize kernels with obvious differences in tolerance to deep sowing may be determined by the genetic characteristics of th...

Embodiment 3

[0078] The invention provides a method for evaluating the maximum elongation characteristics of corn mesocotyls and coleoptiles, specifically:

[0079] (1) seed selection;

[0080] (2) field planting of corn materials;

[0081] (3) Analysis of physical characteristics of corn inbred seeds;

[0082] (4) Analysis of nutritional quality of corn inbred seeds;

[0083] (5) Observation on the maximum elongation characteristics of mesocotyls and coleoptiles of maize inbred lines;

[0084] (6) A method for evaluating the maximum elongation characteristics of maize mesocotyls and coleoptiles, namely: (1) the cumulative mesocotyl length and glycine content, lysine content, glutamic acid content, arginine content of 12-day-old corn Acid content, leucine content, isoleucine content, tyrosine content, methionine content, alanine content, valine content, seed density, threonine content, aspartic acid content, etc. 13 There was a significant correlation between the three traits, and the ...

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Abstract

The invention provides a method for evaluating the maximum elongation characteristics of the mesocotyl and the coleoptile of Zea mays. The method comprises the following steps of: (1) carefully choosing Zea mays seeds; (2) carrying out field planting of Zea mays materials; (3) analyzing the physical characteristics of Zea mays selfing line seeds; (4) analyzing the nutrient quality of the Zea mays selfing line seeds; (5) observing the maximum elongation characteristics of the mesocotyl and the coleoptile in the Zea mays selfing line; and (6) analyzing a result. The method disclosed by the invention takes the Zea mays selfing line seeds with different deep-sowing tolerance characteristics as a test material, the maximum elongation characteristics, which perform a main decisive effect for the deep-sowing tolerance characteristics of the Zea mays, of the mesocotyl/ coleoptile/ seedling length in the corresponding Zea mays selfing line are observed so as to obtain the 12-day-old accumulated mesocotyl length/ coleoptile length/ sum of the mesocotyl and the coleoptile/ ratio of the mesocotyl to the coleoptile of the Zea mays selfing line as well as 22-day-old accumulated seedling length of the Zea mays selfing line during the maximum elongation characteristics of the mesocotyl/ coleoptile/ seedling length, optimal multiple regression equations between the physical characteristics and the nutrient quality characteristics of the seed and the above 12-day-old accumulated mesocotyl length/ coleoptile length/ sum of the mesocotyl and the coleoptile/ ratio of the mesocotyl to the coleoptile of the Zea mays selfing line as well as 22-day-old accumulated seedling length are established, so that the potential maximum elongation characteristics of the mesocotyl, the coleoptilea and the seedling length of different Zea mays selfing lines can be quickly, nondestructively, objectively and accurately predicted, the Zea mays selfing line with good deep-sowing tolerance characteristics can be screened, and a technical reference is provided for quick screening and breeding application of the germplasm resources of the Zea mays selfing line with the good deep-sowing tolerance characteristics.

Description

technical field [0001] The invention belongs to the technical field of breeding of new agricultural varieties, and in particular relates to a method for evaluating the maximum elongation characteristics of maize mesocotyls and coleoptiles, and screening maize germplasm resistant to deep sowing by utilizing the maximum elongation characteristics of maize mesocotyls and coleoptiles resource method. Background technique [0002] Corn (Zea mays) grains are rich in more than 300 metabolites such as starch, crude protein, and crude fat, which can be used as important food for humans, animal feed, and industrial raw materials, as well as important commodities. Maize kernel size is a key and relatively stable life history characteristic of maize, which can reflect the quantity, amount and distribution of nutrients stored in the kernel to a certain extent. There is a large genetic variation between maize and nutritional quality, both of which can affect maize seed vigor, germination...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 赵小强钟源石菁周文期
Owner GANSU AGRI UNIV
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