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Method for cultivating catharanthus roseus transgenic plants induced by agrobacterium tumefacien

A technology of Agrobacterium tumefaciens and periwinkle, applied in botany equipment and methods, horticultural methods, genetic engineering, etc., can solve problems such as the reduction of TIAs content, cell culture metabolites and genetic instability, and application value limitations.

Active Publication Date: 2010-03-10
成都上交致远生物科技有限公司
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Benefits of technology

The technical effect of this patented method is that it allows scientists to create new plants by manipulating certain genes involved in their growth process or producing them through bioremediation techniques. This results in improved plant properties such as increased yield over time compared to traditional methods like crop improvement programs.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving the amount or purity of certain types of chemical compounds called volatile flavonoids found naturally occurring throughout various parts of plants like VINCA. These include α-,β-unsaturated carboxylate esters from citrus fruits, pteridins, lignanolides, coumarinols, cisplatin, tetrahydrocannabinoceae, vanillimodalamide, etc., among others.

Method used

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  • Method for cultivating catharanthus roseus transgenic plants induced by agrobacterium tumefacien
  • Method for cultivating catharanthus roseus transgenic plants induced by agrobacterium tumefacien
  • Method for cultivating catharanthus roseus transgenic plants induced by agrobacterium tumefacien

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Experimental program
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Effect test

Embodiment

[0030] (1) Cultivation of periwinkle aseptic seedlings

[0031] The periwinkle seeds used in the present invention are purchased from PanAmerican Seed Company, Pacifica Cherry Red (PCR). The periwinkle seeds were sterilized with 75% alcohol for 1 min, 20% NaClO for 5 min, rinsed with sterile water for 3 times, blotted the excess water with filter paper, then inoculated on MS medium, cultured for 3-4 days, and cut to obtain 5 mm long Bacterin hypocotyls.

[0032] Culture room conditions: temperature (25±2)°C, light time 16h / d, light intensity 2,000lx.

[0033] (2) Preparation of Agrobacterium tumefaciens bacterial liquid

[0034] Plant expression vector pCAMBIA2300-GUS (such as figure 1) as the test material, which is preserved by the Plant Biotechnology Research Center of Shanghai Jiaotong University. The expression vector can be obtained through publicly available commercial channels, for example, it can be purchased from CAMBIA Company in Australia, and the vector number...

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Abstract

A method for cultivating catharanthus roseus transgenic plants induced by agrobacterium tumefacien comprises the steps as follows: taking aseptic seedling of the catharanthus roseus, and obtaining hypocotyl of the aseptic seedling; preparing bacteria solution of the agrobacterium tumefacien and utilizing the agrobacterium tumefacien for inducing transformation of the hypocotyl of catharanthus roseus; taking and placing the hypocotyl on a callus induction culture medium for culture to obtain a callus; inoculating the callus onto a differential culture medium for culture to obtain a green callus; inoculating the green callus onto an indefinite bud induction culture medium for culture to obtain an indefinite bud; and taking and placing the indefinite bud into a radication culture medium for culture to obtain the catharanthus roseus transgenic plants. In the method, a stable genetic transformation system of the catharanthus roseus induced by the agrobacterium tumefacien is established, therefore the method lays a foundation for further study on metabolic pathway control net and for the improvement of TIAs content, and can be further applied to gene engineering seed breeding for producing TIAs metabolite.

Description

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Claims

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Application Information

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Owner 成都上交致远生物科技有限公司
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