Fast and efficient agrobacterium-mediated rice stem tip genetic transformation method

Abstract

The invention discloses a fast and efficient agrobacterium-mediated rice stem tip genetic transformation method. The method comprises the following major steps: directly soaking mature rice seeds in tap water, accelerating germination, then transversely cutting the coleoptiles to expose stem tips as transformation receptors, performing genetic transformation by virtue of agrobacterium mediation without sterilization treatment, putting the transformed materials under an appropriate environment to allow plants to grow, and carrying out transgenic screening and identification to obtain the transgenic rice plants. According to the fast and efficient agrobacterium-mediated rice stem tip genetic transformation method, callus induction and a series of subsequent tissue culture processes are avoided, the defects of materials affected by bacteria, difficult regeneration, vitrification, browning, low survival rate of transplanted tissue cultured seedlings and the like in the tissue culture process are avoided, the rice genetic transformation cost is reduced, the restriction of the rice genotype to the transformation efficiency is effectively overcome, and a large quantity of transgenic rice plants can be obtained in short time; the fast and efficient agrobacterium-mediated rice stem tip genetic transformation method has a significant value in good-quality rice culture and rice genetic transformation research.

Description

technical field [0001] The invention belongs to the fields of agricultural biotechnology and plant genetic engineering, in particular to a fast and efficient Agrobacterium-mediated rice transformation method. Background technique [0002] Rice is one of the most important food crops in the world. More than half of the world's population depends on rice as a staple food, and its stable supply directly affects the global food balance. Breeding a variety of high-quality rice is one of the ways to solve the increasingly prominent food and environmental problems. Conventional rice hybrid breeding has a long cycle and slow results, and has the disadvantages of gene linkage and reproductive isolation. With the help of mutagenesis breeding under some physical and chemical conditions, it is difficult to control the direction of mutagenesis, and the beneficial mutation rate is low. Obtaining hybrid cells by means of cell fusion, cultivating and developing into plant cell engineering...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the needs in the above-mentioned fields, the present invention provides a genetic transformation method, which avoids the cumbersome technology, strict disinfection and aseptic conditions in plant tissue culture that traditional rice genetic transformation must rely on, long cultivation period, high economic investment, and There are high requirements on the state and genotype of the material. Different materials often need to explore different tissue culture conditions, and even fail to obtain a series of defects such as ideal results. Establish an efficient and rapid Agrobacterium-mediated genetic transformation of rice shoot tips method

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