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SNP (single nucleotide polymorphism) molecular markers related to salt tolerance and hypoxia tolerance of rice and application of SNP molecular markers

A salt tolerance, rice technology, applied in the fields of rice breeding and molecular genetics, can solve the problems of lack of seedlings, irregular seedling emergence, and restrictions on the development of direct seeding rice

Pending Publication Date: 2019-02-22
INST OF CROP SCI CHINESE ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, environmental conditions such as low oxygen, low temperature and high salinity during direct seeding of rice have caused irregular seedling emergence and lack of seedlings, which limit the development of direct direct rice.

Method used

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  • SNP (single nucleotide polymorphism) molecular markers related to salt tolerance and hypoxia tolerance of rice and application of SNP molecular markers
  • SNP (single nucleotide polymorphism) molecular markers related to salt tolerance and hypoxia tolerance of rice and application of SNP molecular markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1. Acquisition and Identification of SNP Molecular Markers Closely Linked to Rice Salt Tolerance and Low Oxygen Germination

[0046] 1. Acquisition of SNP molecular markers closely linked to rice salt tolerance and low oxygen tolerance germination

[0047] 1. From the 3,000 global rice core germplasm resources that have been deeply sequenced (3K RGP.The 3,000RiceGenome Project[J].GigaScience,2014,3:7), according to the growth period, genotype, geographical origin and rice type, etc. characteristics, and combined with other agronomic traits such as plant height and seed setting rate, 582 rice materials with rich genetic variation were screened and used as test materials, including 372 indica rice, 172 japonica rice, 16 Aro and 14 Aus and Admixed 8 servings.

[0048]2. In the laboratory of the Institute of Crop Science, Chinese Academy of Agricultural Sciences, investigate the relative length of coleoptiles of 582 germplasm resources under normal conditions and u...

Embodiment 2

[0056] Example 2, Application of SNP Molecular Markers Closely Linked to Rice Salt Tolerance and Low Oxygen Germination

[0057] 1. Detection of relative length of coleoptile

[0058] In the related population, 20 germplasm resources with significant differences in the relative length of coleoptiles under low-oxygen and high-salt stress conditions were randomly selected as experimental materials, and all 20 materials were recorded in the literature "Wang Wensheng et al. Accessions of Asian cultivated rice. Nature, 2018, 557:43-49.", the public can obtain from the applicant, the biological material is only used for repeating the relevant experiments of the present invention, and cannot be used for other purposes. The relative length of the rice coleoptile was detected according to the method of 2 in step 1 of Example 1. The results are shown in Table 2.

[0059] 2. Genotype detection

[0060] The genotype of the rice to be tested was detected according to the method in Step ...

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Abstract

The invention discloses SNP (single nucleotide polymorphism) molecular markers related to salt tolerance and hypoxia tolerance of rice and application of the SNP molecular markers. The SNP molecular markers provided by the invention are located at the 26th, 874th and 154th sites of rice chromosome 1, and the polymorphism is A or C. The SNP molecular markers are significantly correlated with the length of coleoptiles in rice under hypoxia and high salt germination conditions. The relative length of coleoptiles in a rice variety with a genotype being AA at the sites is significantly greater thanthat of a rice variety with the genotype being CC under hypoxia and high salt germination conditions. The invention also designs PCR primers based on the SNP sites, and the nucleotide sequences of the primers are shown in sequence 1 and sequence 2. The SNP molecular markers provided by the invention are verified in natural populations that the markers can effectively predict the hypoxia and highsalt germination of rice, and thus can be used for marker-assisted breeding of salt tolerance, hypoxia tolerance and direct seeding molecular breeding in rice.

Description

technical field [0001] The invention relates to the fields of rice breeding and molecular genetics, in particular to a SNP molecular marker related to rice salt tolerance and hypoxia tolerance and its application, in particular to a SNP molecular marker on the No. 1 chromosome of rice genome that is related to rice salt tolerance and hypoxia tolerance. SNP molecular markers significantly associated with oxygen germination traits and their applications. Background technique [0002] Rice is one of the three most important food crops in the world and is the main source of food for 2 / 3 of the world's population. With the rapid growth of population and economy, food production is facing enormous development pressure, and safe production of rice is crucial to China's sustainable development. In recent years, with the development of the economy, the increase of labor costs and the popularization of agricultural mechanization, direct-directed rice has become a new development dire...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 王文生傅彬英穆罕默德·拉菲克伊·斯拉姆黎志康吴志超徐建龙赵秀琴张帆孙帆
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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