High-molecular wheat glutelin subunit gene, nucleic acid sequence of promoter thereof, and application thereof

A glutenin subunit, high molecular weight technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc.
CN1970765AInactive Publication Date: 2007-05-30SICHUAN AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SICHUAN AGRI UNIV
Publication Date
2007-05-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a coded gene sequence and amino acid sequence with high-expressive activity macromolecular glutelin subunit with sequence as SEQ ID No.1 and SEQ ID No.2 in the biological gene engineering technical domain, which is characterized by the following: providing astopic endosperm promoter with high-expressive activity macromolecular glutelin subunit with sequence as SEQ ID No.3; constructing gene-transmitting carrier; utilizing gene shooting method, agricillin dielectric method and pollen tube channel method to transmit gene to common wheat; culturing superior wheat.
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Description

technical field

[0001] The invention belongs to the technical field of biogenetic engineering, and in particular relates to a high-molecular-weight glutenin subunit gene 1Ay with expression activity and a nucleic acid sequence of an endosperm-specific expression promoter and application thereof. Background technique

[0002] Wheat is one of the most important food crops, accounting for about 17% of the cultivated area of ​​food crops in the world, and is the basic source of human food and nutrition. Wheat endosperm storage proteins can be divided into two categories: Glutenin and Gliadin. According to the difference of SDS-PAGE electrophoretic mobility, gluten can be divided into high molecular weight glutenin subunits (High molecular weight glutenin subunits, HMW-GS) and low molecular weight glutenin subunits (LHW-GS ). High-molecular-weight glutenin subunits are an important storage protein in wheat seeds, and are the main factors that determine the processing and baking...

Claims

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