Explant collecting and inducing method of leaf buds of betula alnoides
An explant and germination technology, applied in botany equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems of different growth phenotypes, it is difficult to achieve high yield and high quality of the population, and hinder the application of plant tissue culture technology, etc. To achieve simple and practical operation, ensure good quality, and ensure consistent results
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[0011] Example 1:
[0012] (1) Selection of good mother trees. In 2005, 10 excellent trees were selected from the 15-year-old Southwestern birch plantation.
[0013] (2) The method of promoting buds at the base of the trunk. In August 2005, weeds and shrubs within a diameter of 5m around the selected mother tree were removed to make the trunk fully exposed to sunlight, and a 6-benzyl adenine (6-BA) solution with a concentration of 5 mg / L was prepared. Use the medical infusion bottle to hang on the trunk at a height of 2m and use the medical infusion tube to maintain the infusion on the cambium at the base of the trunk for 35 days to promote its germination;
[0014] (3) Treatment of explant materials. In October 2005, use scissors to cut the sturdy young leaf buds of 2 to 3 cm in length from the base of the trunk 2m below, and cut off the leaves other than the apical buds as tissue culture explants. A total of 55 leaf buds were collected. The leaf bud explants are first soaked in...
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[0017] Example 2:
[0018] (1) Selection of good mother trees. In 2008, 10 excellent trees were selected from the 5-year-old Southwestern birch plantation.
[0019] (2) The method of promoting buds at the base of the trunk. In October 2008, weeds and shrubs within a diameter of 5m around the selected mother tree were removed to make the trunk fully exposed to sunlight, and a 6-benzyl adenine (6-BA) solution with a concentration of 5 mg / L was prepared. , Use a medical infusion bottle to hang on the trunk at a height of 2m and use a medical infusion tube to maintain the infusion on the cambium at the base of the trunk for 32 days to promote its germination;
[0020] (3) Treatment of explant materials. In December 2008, scissors were used to cut 2 to 3 cm long sturdy young leaf buds that sprouted below 2 m from the base of the trunk, and cut off the leaves other than the terminal buds as tissue culture explants. A total of 40 leaf buds were collected. The leaf bud explants are first...
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