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Explant collecting and inducing method of leaf buds of betula alnoides

An explant and germination technology, applied in botany equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems of different growth phenotypes, it is difficult to achieve high yield and high quality of the population, and hinder the application of plant tissue culture technology, etc. To achieve simple and practical operation, ensure good quality, and ensure consistent results

Inactive Publication Date: 2013-07-17
中国林业科学研究院热带林业实验中心
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AI Technical Summary

Problems solved by technology

At present, the traditional method of sowing seedlings is mainly used in production to cultivate afforestation seedlings. Due to the diversity of genetic materials, the growth phenotypes of seedlings are different after afforestation, and it is difficult to achieve the goal of high-yield and high-quality groups.
The use of plant tissue culture propagation technology can quickly propagate clone seedlings with excellent genes, so that the purpose of high-yield and high-quality population can be achieved after afforestation. However, the collection and induction of explants in the tissue culture process is a key technical link, which often hinders plant tissue culture. The bottleneck of technology application

Method used

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  • Explant collecting and inducing method of leaf buds of betula alnoides

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Experimental program
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Effect test

Embodiment 1

[0012] (1) Selection of excellent mother trees. In 2005, 10 excellent trees were selected in the 15-year-old Betula plantation.

[0013] (2) The method for promoting germination of leaf buds at the base of the trunk. In August 2005, the weeds and shrubs within the diameter of 5m around the selected mother tree were removed, so that the trunk was fully exposed to sunlight, and a 6-benzyladenine (6-BA) solution of 5 mg / L was prepared at the same time , use a medical infusion bottle to hang on the trunk at a height of 2m, and use a medical infusion tube to keep the cambium at the base of the trunk infused for 35 days to promote its germination;

[0014] (3) Processing of explant material. In October 2005, 2-3cm long robust young leaf buds germinated below the trunk base 2m were cut with scissors, and the leaves other than the terminal buds were cut off as tissue culture explants. A total of 55 leaf buds were collected. Leaf bud explants were first soaked in 3% washing powder w...

Embodiment 2

[0018] (1) Selection of excellent mother trees. In 2008, 10 excellent trees were selected in the 5-year-old Southwest Betula plantation.

[0019] (2) The method for promoting germination of leaf buds at the base of the trunk. In October 2008, the weeds and shrubs within the diameter of 5m around the selected mother tree were removed, so that the trunk was fully exposed to sunlight, and a 6-benzyladenine (6-BA) solution with a concentration of 5mg / L was prepared at the same time , use a medical infusion bottle to hang on the trunk at a height of 2m, and use a medical infusion tube to keep the cambium at the base of the trunk infused for 32 days to promote its germination;

[0020] (3) Processing of explant material. In December 2008, scissors were used to cut the 2-3cm long robust young leaf buds that germinated below the base of the trunk 2m, cut off the leaves other than the terminal buds, and used them as tissue culture explants. A total of 40 leaf buds were collected. Le...

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Abstract

The invention discloses an explant collecting and inducing method of leaf buds of betula alnoides, belonging to the technical field of vegetative propagation of plants. The method comprises the following steps of: (1) selecting a betula alnoide superior tree of above five years old as a seed tree; (2) in August-October, clearing weeds and bushes around the seed tree in the diameter range of 5m to ensure that the tree trunk is fully illuminated by sunlight, preparing a 6-benzyladenine solution with the concentration of 5mg / L simultaneously and transfusing the solution into the cambium of the tree trunk base for more than 30 days; (3) in October-December, scissoring and taking 2-3cm long healthy and strong leaf bud produced at 2m below the tree trunk base as the explant, soaking for 20min by using 3% of washing powder, disinfecting the explant for 10-15s by using 75% of alcohol on a super-clean worktable, disinfecting the explant for 7-10min by using 10% of H2O2 and finally washing 5-7 times by using sterile water; and (4) transferring the leaf bud to an induction medium of the explant and culturing for 40-60 days, wherein the induction success rate is more than 50%.

Description

technical field [0001] The invention relates to a method for collecting and inducing leaf bud explants in the tissue culture propagation technology of betula southwest, belonging to the technical field of plant asexual propagation. Background technique [0002] Southwest Betula is an excellent native fast-growing timber tree species of Betulaceae in southern my country. It has strong adaptability and wide uses. It is suitable for cultivating large and medium-diameter timber, but the resources of fine species are very scarce. At present, the traditional method of sowing and raising seedlings is mainly used in production to cultivate afforestation seedlings. Due to the diversity of genetic materials, the seedlings have different growth phenotypes after afforestation, and it is difficult to achieve the goal of high-yield and high-quality groups. The use of plant tissue culture propagation technology can quickly propagate clone seedlings with excellent genes, so as to achieve th...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王小宁谌红辉贾宏炎
Owner 中国林业科学研究院热带林业实验中心
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