The invention discloses a hyaluronic acid stock solution, which comprises, by weight, 4-10% of butanediol, 2-7% of glycerol, 0.05-0.5% of sodium hyaluronate, 0.2-0.8% of hydroxyethyl cellulose, 0.5-2%of betaine, 1-5% of hydrolyzed chitin, 0.3-1.2% of p-hydroxyacetophenone, 0.5-2% of oat-beta-glucan, 0.1-0.8% of a mixture of sclerotium and phenoxyethanol,0.2-1% of 1, 2-hexanediol, water and the balance; the sum of the weight percentages of the above components is 100%. According to the hyaluronic acid stock solution, a hyaluronic acid precursor with small molecular weight is permeated into skin, so that the synthesis speed of the hyaluronic acid in the skin is promoted; and moreover, the hyaluronic acid stock solution is fresh and non-greasy in texture, and is water-supplementing and moisture-preserving. The invention also discloses a preparation method of the hyaluronic acid stock solution, which has simple operation and lower cost and is suitable for most production equipment on themarket.