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Tissue culture fast propagation method of Eucalyptus pellita

A technique for tissue culture rapid propagation and eucalyptus roughum, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effects of low operation difficulty, high survival rate, and stable genetic traits

Active Publication Date: 2016-03-09
广西壮族自治区国有东门林场
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, there is no relevant literature report on the use of excellent single-plant germination strips of excellent Eucalyptus rugosa lines as explants, directly carrying out tissue culture without going through the callus approach, and quickly obtaining a large number of Eucalyptus rugosa seedlings with stable genetic characteristics

Method used

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  • Tissue culture fast propagation method of Eucalyptus pellita
  • Tissue culture fast propagation method of Eucalyptus pellita
  • Tissue culture fast propagation method of Eucalyptus pellita

Examples

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Effect test

Embodiment 1

[0042] A method for tissue culture and rapid propagation of Eucalyptus rugosa, the steps of which are as follows:

[0043] (1) In the family test forest introduced in Australia, after testing and comparison, select an excellent family of Eucalyptus rugosa suitable for local growth;

[0044] (2) In the excellent family of Eucalyptus rugosa, select excellent individual plants after comprehensive comparison by measuring diameter at breast height, tree height, stock volume, annual growth, wood properties and dry shape indicators;

[0045] (3) Cut down the fine individual plants at a height of 15cm from the ground to promote germination. When the sprouting strips grow to 12cm, cut the sprouting strips, soak the base in water and bring them back to the laboratory for later use;

[0046] (4) Trim the bud strips to remove leaves and petioles, rinse with running water for 3 minutes, then wash with saturated detergent solution for 10 minutes, and clean with pure water;

[0047] (5) On ...

Embodiment 2

[0055] A method for tissue culture and rapid propagation of Eucalyptus rugosa, the steps of which are as follows:

[0056] (1) In the family test forest introduced in Australia, after testing and comparison, select an excellent family of Eucalyptus rugosa suitable for local growth;

[0057] (2) In the excellent family of Eucalyptus rugosa, select excellent individual plants after comprehensive comparison by measuring diameter at breast height, tree height, stock volume, annual growth, wood properties and dry shape indicators;

[0058] (3) Cut down the fine individual plants at a height of 18cm from the ground to promote germination. When the sprouting strips grow to 15cm, cut the sprouting strips, soak the base in water and bring them back to the laboratory for later use;

[0059] (4) Trim the bud strips to remove leaves and petioles, rinse with running water for 4 minutes, then wash with saturated detergent solution for 10 minutes, and clean with pure water;

[0060] (5) On ...

Embodiment 3

[0068] A method for tissue culture and rapid propagation of Eucalyptus rugosa, the steps of which are as follows:

[0069] (1) In the family test forest introduced in Australia, after testing and comparison, select an excellent family of Eucalyptus rugosa suitable for local growth;

[0070] (2) In the excellent family of Eucalyptus rugosa, select excellent individual plants after comprehensive comparison by measuring diameter at breast height, tree height, stock volume, annual growth, wood properties and dry shape indicators;

[0071] (3) Cut down the fine individual plants at a height of 20cm from the ground to promote germination. When the sprouting strips grow to 18cm, cut the sprouting strips, soak the base in water and bring them back to the laboratory for later use;

[0072] (4) Trim the bud strips to remove leaves and petioles, rinse with running water for 5 minutes, then wash with saturated detergent solution for 10 minutes, and clean with pure water;

[0073](5) On t...

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Abstract

The invention discloses a tissue culture fast propagation method of Eucalyptus pellita. The method comprises the following steps: adopting excellent single plants of an excellent family of Eucalyptus pellita, carrying out stump sprouting on the excellent single plants, carrying out in vitro aseptic bud induction on stems with buds through using obtained sprouts as an explants to directly generate lateral buds or adventitious buds without a callus approach, carrying out shoot proliferation culture on the aseptic buds, carrying out rooting culture, and transplanting rooted seedlings to obtain regenerated plants. The tissue culture fast propagation method of Eucalyptus pellita has the advantages of low operating difficulty, low production cost, high rooting rate (of 90% or above), high survival rate of produced tissue culture plants, and easy large-scale popularization and use, and the regenerated plants obtained by using the method have the advantages of stable result, good repeatability, realization of large-scale popularization and seedling growing, stable hereditary character, and maintenance of excellent hereditary characters of excellent trees.

Description

technical field [0001] The invention relates to the technical field of eucalyptus cultivation, in particular to a method for tissue culture and rapid propagation of eucalyptus roughum. Background technique [0002] Eucalyptus is native to Australia and belongs to the Myrtle family (Mytaceae) Eucalyptus ( Eucalyptus ), Cupberry ( Angophora ), corymbs ( Corymbia ) The collective name of the three genus tree species. A total of 1039 species, subspecies and varieties, Eucalyptus rougha belongs to a species of Eucalyptus. Because of its fast growth, short rotation period, wide adaptability, wide application and high economic benefits, eucalyptus has become one of the three world-recognized plantation tree species. my country has been introduced since 1890 and has a history of more than 120 years. The introduction was mainly used for garden planting at first, and by 1973, a more systematic provenance family experiment was carried out. In 1982, the Sino-Australian technical...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/008
Inventor 唐再生苏勇莫继有李丽芳石前梁庭辉黎怀玲熊涛张磊王建忠陈东林梁秀莉兰俊
Owner 广西壮族自治区国有东门林场
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