Tabebuia chrysantha tissue culture and rapid propagation method

A method of tissue culture of Suzuki chinensis, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effects of easy disinfection, maintaining excellent genetic traits, and rapid germination

Active Publication Date: 2017-05-17
钦州市林业科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on domestic research on propagation of P. chrysantha by tissue culture technique

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) The preparation of the culture medium: including the basic medium and the composition of the culture medium at each stage and the weight per liter, of which:

[0028] 1. Basic medium: MS medium;

[0029] 2. Initial culture medium: MS+6-BA0.4mg / L+NAA0.2mg / L+sucrose 30g / L, agar powder 4.2g / L, pH=5.8;

[0030] 3. Bud differentiation and proliferation medium: MS+6-BA1.2mg / L+IAA0.3-0.5mg / L+L-cysteine ​​25mg / L+ sucrose 30g / L, agar powder 4.2g / L, pH = 5.8;

[0031] 4. Rooting medium: 1 / 2MS+IBA0.8mg / L+activated carbon 0.2g / L+sucrose 20g / L, agar powder 4.5g / L, pH=5.8;

[0032] (2) Processing and disinfection of explants: take the seeds of P. chinensis as explants, peel off the outer seed shell, get the seed kernels, and wash them with 0.1% HgCl on the ultra-clean workbench. 2 Disinfect the solution for 8 minutes, then wash it with sterile water for 4-5 times, and drain it for later use;

[0033] (3) Initial culture: inoculate the sterilized explants on the primary cultur...

Embodiment 2

[0038] (1) The preparation of the culture medium: including the basic medium and the components of the culture medium at each stage of cultivation and the weight contained in each liter, wherein:

[0039] 1. Basic medium: MS medium;

[0040]2. Initial culture medium: MS+6-BA0.4mg / L+NAA0.2mg / L+sucrose 30g / L, agar powder 4.2g / L, pH=5.8;

[0041] 3. Bud differentiation and proliferation medium: MS+6-BA1.6mg / L+IAA0.3-0.5mg / L+L-cysteine ​​25mg / L+ sucrose 30g / L, agar powder 4.2g / L, pH = 5.8;

[0042] 4. Rooting medium: 1 / 2MS+IBA1.0mg / L+ activated carbon 0.2g / L+ sucrose 20g / L, agar powder 4.5g / L, pH=5.8;

[0043] (2) Processing and disinfection of explants: take the seeds of P. chinensis as explants, peel off the outer seed shell, get the seed kernels, and wash them with 0.1% HgCl on the ultra-clean workbench. 2 Disinfect the solution for 10 minutes, then wash it with sterile water for 4-5 times, and drain it for later use;

[0044] (3) Initial culture: inoculate the sterilized e...

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PUM

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Abstract

The invention discloses a tabebuia chrysantha tissue culture and rapid propagation method. The tabebuia chrysantha tissue culture and rapid propagation method comprises the steps of treatment and disinfection of an explants, acquisition of aseptic seedlings, differentiation and proliferation of aseptic buds and culture and transplanting of rooting seedlings. A propagation method and a culture medium formula of the invention are used for carrying out tabebuia chrysantha tissue culture, the annual growth coefficient of the aseptic buds reaches 3.012, and the rooting rate reaches 97 percent. The tabebuia chrysantha tissue culture and rapid propagation method is provided by using tabebuia chrysantha seeds as the explants, an effective path is provided for seedling breeding of tabebuia chrysantha, and large-scale production requirements of the seedling breeding are met.

Description

technical field [0001] The invention belongs to the technical field of asexual propagation of seedlings, and in particular relates to a rapid propagation method of Suzuki chrysanthemum tissue culture. Background technique [0002] Tabebuia chrysantha is a deciduous tree belonging to the Bignoniaceae family, native to Mexico, Central America, and South America. It has various colors and is a tree that changes its appearance with the seasons. It has the unique charm of spring flowers, summer fruits, autumn green and winter dryness. In the spring of flowering, the flowers open first and the leaves open, the corolla is bell-shaped, and the trees are golden when they are in full bloom. It is quite spectacular and has excellent ornamental value. It has been widely used in gardens and courtyard greening in South China for more than ten years. Greenery and street trees. In addition, Suzuki chrysalis has strong adaptability, extensive management and high planting profits, and is ve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈丽文时群杨利平陈乃明何贵整王华宇蔡林梁刚樊东函陈艳杨琼
Owner 钦州市林业科学研究所
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