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Tissue culture rapid propagation method of eucalyptus fine-breed urophylla*E.camaldulensis DH191-7

A technology of tissue culture rapid propagation and red eucalyptus, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of no clone name, less research on seedling cultivation of the red eucalyptus group, and unclear sources, etc., to achieve Good repeatability, stable test results, and stable genetic traits

Inactive Publication Date: 2017-10-20
广西壮族自治区国有东门林场
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] At present, there are a lot of researches on the tissue culture of Eucalyptus erythra in China, but there are few studies on the cultivation of Eucalyptus erythra group. In 2007, Zhang Yuanhua et al. 2 、D 6 、D 5 The tissue culture rapid propagation was successfully carried out; in 2006, Chen Jianyong et al. successfully carried out the tissue culture rapid propagation of Eucalyptus, but there was no specific clone name, and the source was not clear; in 2006, Zhang Lianshui et al. The breeding was successful, but DH201 is a hybrid offspring of Eucalyptus grandis and Eucalyptus spp., not G.

Method used

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  • Tissue culture rapid propagation method of eucalyptus fine-breed urophylla*E.camaldulensis DH191-7
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  • Tissue culture rapid propagation method of eucalyptus fine-breed urophylla*E.camaldulensis DH191-7

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Experimental program
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Effect test

Embodiment 1

[0045] The specific method for obtaining regenerated plants by tissue culture and rapid propagation of the improved Eucalyptus eucalyptus DH191-7 is as follows:

[0046] (1) In the first-generation clone stand of Eucalyptus eucalyptus DH191-7, the diameter at breast height, tree height, wood properties and dry shape indicators were measured, and excellent individual plants were selected after calculation and analysis, as a fast tissue culture method. Propagated breeding object, determine that the breeding object is the first-generation ramet of the original strain of the DH191-7 clone.

[0047] (2) Cut down the excellent individual DH191-7 plants at a height of 10-15cm from the ground to promote germination. When the sprouting strips grow to 10-15cm, cut the sprouting strips, soak the base in water, and bring them back to the laboratory for later use.

[0048] (3) Trim the bud strips to remove the leaves, wash them with running water for 30 minutes, then wash them with a satur...

Embodiment 2

[0056] (1) In the first-generation clone stand of Banwei red eucalyptus DH191-7 in 25 forests of Dongmen Forest Farm, through the measurement of diameter at breast height, tree height, height under branches and trunk shape, etc., the excellent individual plants were selected after calculation and analysis , as the breeding object of tissue culture rapid propagation, it is determined that the breeding object is the first-generation ramet of the original DH191-7 clone.

[0057] (2) In the morning of spring and no rain, cut down the fine DH191-7 plants at a height of 15cm from the ground to promote germination. After 25 days, when the germination strips grow to 10-15cm, choose the ones with more than 3 consecutive days of sunny days. In the morning, cut the sprouting strip near the rhizome, soak the base in water, and bring it back to the laboratory for later use;

[0058](3) Trim the bud strips to remove the leaves, keep the petioles, rinse with running water for 30 minutes, the...

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Abstract

The invention discloses a tissue culture rapid propagation method of eucalyptus fine-breed urophylla*E.camaldulensis DH191-7. Stump germination promotion is performed on a superior tree of a DH191-7 clone, and a stem segment of a sprouting band latent bud is used as an explant to perform induction in vitro on a germ-free bud. A side bud is sprouted directly from the stem segment without a callus tissue, and subculture multiplication cultivation, rooting cultivation and rooted seedling transplantation are performed on the germ-free bud to obtain a regeneration plant. As the eucalyptus fine breed is used as a reproduction target, and seedlings are directly formed in the way of performing induction in vitro on the germ-free bud by a bud organ, the fine hereditary feature of the superior tree can be completely kept in the regeneration plant, and the regeneration plant is not easy to mutate; the method is low in operation difficulty, low in production cost, high in nursery stock root yield, high in transplantation survival rate, stable in result and good in repeatability; and the method can be used for massive industrialized seedling production.

Description

technical field [0001] The invention relates to the technical field of eucalyptus cultivation, in particular to a method for tissue culture and rapid propagation of Eucalyptus eucalyptus DH191-7, a fine species of eucalyptus. Background technique [0002] Eucalyptus is native to Australia and belongs to the Myrtle family (Mytaceae) Eucalyptus ( Eucalyptus ), Cupberry ( Angophora ), corymbs ( Corymbia ) The collective name of the three genus tree species. There are a total of 1039 species, subspecies and varieties, among which there are as many as 945 species of Eucalyptus, with a large geographical distribution span and large differences in performance. Introduced and cultivated in more than 100 countries in the world, they all show excellent characteristics such as strong adaptability, fast growth, high yield, and short rotation period. Eucalyptus wood can be used in vehicles, buildings, mines, wood-based panels, and is an important raw material for the pulp and paper...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 唐再生李丽芳张磊王建忠熊涛邱炳发陈德文莫继有兰俊黎怀玲陈东林陈远龙沈云吴兵庞贞武黄全东梁秀莉韦炳俭吴满芬俸荣娣
Owner 广西壮族自治区国有东门林场
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