Factory-like rapid propagation method for test-tube seedlings of dendranthema morifolium

A test-tube seedling, rapid propagation technology, applied in the directions of gardening methods, botanical equipment and methods, gardening tools/equipment, etc., to achieve the effects of high efficiency, land saving, and fast reproduction

Inactive Publication Date: 2014-03-26
INST OF GARDENING ANHUI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After retrieval, no patents related to Bo Chrysanthemum's industrial seedling cultivation technology have been declared at home and abroad

Method used

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  • Factory-like rapid propagation method for test-tube seedlings of dendranthema morifolium
  • Factory-like rapid propagation method for test-tube seedlings of dendranthema morifolium
  • Factory-like rapid propagation method for test-tube seedlings of dendranthema morifolium

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example 1

[0032] (1) Collection and pretreatment of Bo chrysanthemum explants:

[0033] For Bochrysanthemum plants that grow robustly in the field and are free from diseases and insect pests, when they bloom in autumn, choose flower buds wrapped with sepal diameters ranging from 0.2-0.3cm as inoculation materials, pick branches with flower buds, place them in a sterile environment, and put them under ultraviolet light Irradiate for 15-20 minutes, then soak the flower buds in saturated washing powder for 15 minutes, and rinse with running water for 30 minutes. In addition, an appropriate bag cover can be added to keep the buds clean before picking.

[0034] (2) disinfection of chrysanthemum explants:

[0035] Disinfection and inoculation of explants: soak the pretreated flower buds in 75% alcohol for 30 seconds, then disinfect them with 0.1% mercury chloride for 8-10 minutes, rinse them with sterile water for 3-4 times, and absorb the surface moisture with sterile filter paper . Under...

Embodiment 2

[0042] Embodiment 2: The method for the rapid propagation of Bo chrysanthemum industrialized test-tube plantlets, including the rapid propagation process of Bo chrysanthemum tissue culture test-tube plantlets and the transitional culture process of test-tube plantlets.

[0043]The rapid propagation process of the described Bo chrysanthemum tissue culture test-tube plantlet comprises:

[0044] 1) Collection and pretreatment of Bochrysanthemum explants: Take Bochrysanthemum explants that grow vigorously and are free from diseases and insect pests. When they bloom in autumn, choose unopened flower buds wrapped with sepals as inoculation materials, and pick flower buds with a diameter range of 0.3cm. placed in a sterile environment, irradiated with ultraviolet light for 20 minutes, then soaked the flower buds in saturated washing powder for 15 minutes, and rinsed with running water for 30 minutes;

[0045] 2) Disinfection and inoculation of explants: Disinfection of explants: soak...

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Abstract

The invention discloses a factory-like rapid propagation method for test-tube seedlings of dendranthema morifolium. The method comprises the following steps: by taking a flower bud of dendranthema morifolium as an explant, inducing dedifferentiation and redifferentiation; obtaining the test-tube seedlings through a tissue culture technique; carrying out transitional planting in a vermiculite matrix or a matrix with a ratio of peat to vermiculite to perlite being 33% to 33% to 33% to obtain a shoot with 3-5 leaves and height of 15-20cm after about 30 days; and transplanting to a land for growing field crops, thus harvesting in the current year. By adopting the technical scheme, the seedlings are fully originated from a same plant which accords with inheritable character, so that virus accumulation is reduced, the period is short and the effect is achieved fast. Genuine medicinal material dendranthema morifolium is convenient to store and quick to popularize as a conventional resource, and the method is an effective method suitable for factory-like production of the test-tube seedlings.

Description

technical field [0001] The invention relates to a method for rapid propagation of Bo chrysanthemum industrialized test-tube seedlings, and belongs to the fields of agricultural biotechnology and tissue culture rapid propagation of medicinal plants. Background technique [0002] Bo chrysanthemum [Dendranthem a morifolium (Rama.t) Tzve.l-Boju.cv.nov.] is a Compositae plant, mainly produced in Bozhou, Guoyang, Anhui, with a cultivation history of 200 to 300 years. Hang chrysanthemum and Gong chrysanthemum are called four medicinal chrysanthemums. "Dictionary of Chinese Medicine" contains: "white chrysanthemum is produced in Bozhou, Anhui, and it is called Boju, and its quality is the best." The same is true of "Chinese Medicine Records". The 20th century traditional Chinese medicine masterpiece "Zhonghua Materia Medica" also claims that Boju and Chuju are the best medicines. In recent years, due to serious species degradation, low efficiency and market reasons, the planting ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 江芹董玲廖华俊宁志怨李卫文陈静娴汤婷婷
Owner INST OF GARDENING ANHUI ACADEMY OF AGRI SCI
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