Compound premix for enhancing health of fish intestinal tracts and preparation method and application thereof
A technology for premix and fish, applied in the direction of bacteria used in food preparation, application, animal feed, etc., can solve the problems affecting the comprehensive economic benefits of farmed fish, achieve enhanced immune defense function, good regulation ability, and promote Effect of repairing damaged intestinal villi
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Embodiment 1
[0029] In terms of parts by mass, weigh the total content of the number of bacteria ≥ 10 12 25 cfu of solid plant source Streptococcus faecalis powder, total bacteria content ≥10 13 28 parts of cfu solid lactobacillus powder, 27 parts of compound Chinese medicine extract, 20 parts of flour. Then, the raw material components are fully mixed and passed through an 80-mesh pulverizer to prepare a mixture, and dried at 25°C until the moisture content is 9%, and the prepared mixture is collected as the compound premix of the present invention.
[0030] The solid plant-source Streptococcus faecalis powder is prepared by the following steps: inoculate the plant-source Streptococcus faecalis species into a lactic acid bacteria liquid medium, perform anaerobic fermentation at a constant temperature of 35°C for 48 hours, centrifuge at 5000rpm for 10 minutes to collect the bacteria, and neutralize PBS After diluting the buffer solution, centrifuge at 5000rpm for 10 minutes to collect the...
Embodiment 2
[0034] In terms of parts by mass, weigh the total content of the number of bacteria ≥ 10 12 28 cfu of solid plant source Streptococcus faecalis powder, total bacteria content ≥10 1325 parts of cfu solid lactobacillus powder, 25 parts of compound Chinese medicine extract, and 22 parts of flour. Then, the raw material components are fully mixed and passed through an 80-mesh pulverizer to prepare a mixture, and dried at 25°C until the moisture content is 10%, and the prepared mixture is collected as the compound premix of the present invention.
[0035] The solid plant-source Streptococcus faecalis powder is prepared by the following steps: inoculate the plant-source Streptococcus faecalis species into a lactic acid bacteria liquid medium, perform anaerobic fermentation at a constant temperature of 35°C for 48 hours, centrifuge at 5000rpm for 10 minutes to collect the bacteria, and neutralize PBS After diluting the buffer solution, centrifuge at 5000rpm for 10 minutes to collect...
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