Serum specific biomarker used for diagnosing primary liver cancer and screening method and application thereof

Abstract

The invention relates to a serum specific biomarker used for diagnosing primary liver cancer and a screening method and an application thereof. According to the invention, a Boyden chamber is used forseparating extension and soma of liver cancer transfer cells, from a subcellular structure, an improved Boyden chamber method is used for separating extension and soma of liver cancer transfer cells,and the separating results can be verified by using the technologies such as Western-blot, RNA dyeing and immunofluorescence. The differential expression condition in HCCLM3 liver cancer cell extension and soma is researched, minimal RNA-related to a HCC infiltration transferring process is screened, and clinical experiment proves that change of individual serum has-let-7c-3p has high sensitivityand specificity on HCC diagnosis. The serum specific biomarker provides a novel diagnosis means for clinical diagnosis of the primary liver cancer, and has certain latent clinical value on HCC treatment and pathogenesis monitoring, through a ROC curve of let-7c-3p as well as the ROC curve of let-7c-3p and CEA combination, let-7c-3p and AFP combination, and let-7c-3p and AFP and CEA combination, sensitivity and specificity are increased, and diagnosis value can be obviously increased.

Description

technical field [0001] The present invention specifically relates to the field of molecular biology technology, in particular to a serum-specific biomarker for diagnosing primary liver cancer, a screening method and application. Background technique [0002] Hepatocellular carcinoma (HCC) is the fifth most common malignant tumor worldwide. Although great progress has been made in the diagnosis and treatment of HCC in recent years, the 5-year survival rate of HCC patients is still quite low due to the frequent clinical recurrence and metastasis of HCC. The occurrence and development of HCC is a typical multi-stage process: first, liver tissue is exposed to external stimuli (hepatitis B virus (HBV) or hepatitis C virus (HCV) infection, aflatoxin B1 intake or alcohol abuse etc.) develop chronic hepatitis or cirrhosis, and then, after going through a series of hyperplasia and dysplasia stages, finally acquire the malignant phenotype of intrahepatic metastasis and distant metast...

AI Technical Summary

Problems solved by technology

[0004] Although some progress has been made in the basic and clinical research of HCC in recent years, the mechanism of HCC metastasis and recurrence has not yet been elucidated.

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