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A kit for detecting serum-specific miRNA in Yin-deficiency and upper-fire constitution and its application

A specific, kit-based technology applied in the field of serum biomarkers to achieve high-accuracy results

Active Publication Date: 2021-06-08
李继承
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

miRNA exists stably in serum and plasma, and is suitable as a candidate biomarker for disease, and finding disease biomarkers from serum has always been a research hotspot, but the use of miRNA as a biomarker for TCM syndromes is still scarce. There are reports

Method used

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  • A kit for detecting serum-specific miRNA in Yin-deficiency and upper-fire constitution and its application
  • A kit for detecting serum-specific miRNA in Yin-deficiency and upper-fire constitution and its application
  • A kit for detecting serum-specific miRNA in Yin-deficiency and upper-fire constitution and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Composition of the kit for detecting serum-specific miRNA in Yin-deficiency and upper-fire constitution

[0057] In this example, the kit for detecting serum-specific miRNA in yin-deficiency and upper-fire constitution includes 3 PCR primers for serum miRNA, 1 PCR primer for internal reference miRNA, RNA extraction solution, reverse transcription reaction solution, Fluorescent quantitative RT-PCR reaction solution and small RNA adsorption column.

[0058] In this embodiment, the three serum miRNAs are hsa-miR-221-3p, hsa-miR-30b-5p and hsa-miR-142-3p respectively, and the PCR primers for the three serum miRNAs include hsa-miR- 221-3p PCR primers, hsa-miR-30b-5p PCR primers and hsa-miR-142-3p PCR primers;

[0059] The PCR primer sequences of the 3 serum miRNAs are as follows:

[0060] Primer for hsa-miR-221-3p: 5'-GCGCAGCTACATTGTCTGCTGGGTTTC-3',

[0061] Primer for hsa-miR-30b-5p: 5'-CGCGCGTGTAAACATCCTACACTCAGCT-3',

[0062] Primer for hsa-miR-142-3p: 5'-CC...

Embodiment 2

[0071] Example 2 Establishment of Logistic stepwise regression model for detection of yin-deficiency and upper-fire constitution serum-specific miRNA kit

[0072] Serum-specific miRNA kits for detecting Yin-deficiency and upper-fire constitution were used to detect hsa-miR-221-3p, hsa-miR-30b-5p and hsa-miR-142-3p in the serum of people with Yin-deficiency and upper-fire constitution and normal constitution level of expression.

[0073] (1) Sample collection

[0074] According to the TCM disease diagnostic criteria and syndrome classification diagnostic criteria for aphthous sores in the TCM industry standard of the People's Republic of China "Diagnosis and Curative Effect Criteria of TCM Diseases and Syndromes" issued in 1994, those who meet the following clinical symptoms are listed as yin-deficiency lit constitution: 1) The main symptoms are single or several ulcers with a diameter of 3 to 5 mm in the oral mucosa, and burning pain as the main symptoms; 2) the onset is fast...

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Abstract

The invention discloses a kit for detecting serum-specific miRNA for Yin deficiency and inflamed constitution, and belongs to the field of serum biological markers. The kit includes 3 PCR primers for serum miRNA. The kit for detecting serum-specific miRNA for Yin deficiency and inflamed constitution provided by the present invention adopts three serum miRAs as diagnostic markers, and quantifies the above markers to identify Yin deficiency and inflamed constitution, which can replace the traditional The TCM diagnostic methods of smelling, asking, and cutting combine the diagnosis of TCM syndromes with modern molecular biology to achieve the standardization of diagnosis; and the kit for detecting serum-specific miRNAs of Yin deficiency and inflamed constitution, through the Three serum miRNAs were quantified at the same time to identify Yin-deficiency inflamed constitution. The diagnostic sensitivity reached 95.7% and the specificity reached 73.6%, which was higher than the diagnostic characteristics of a single miRNA.

Description

technical field [0001] The invention relates to the field of serum biological markers, in particular to a kit for detecting serum-specific miRNA in yin-deficiency and upper-fire constitution and its application. Background technique [0002] Yin deficiency is a common sub-health state, manifested as dry mouth, dry eyes, pain, dry stool, upset and irritable, or accompanied by recurrent mouth sores, swollen gums, swollen throat and other inflammatory symptoms , commonly known as getting angry. Although getting angry from yin deficiency has not yet reached the clinical diagnosis standard of the disease, as a sub-health state, it seriously affects people's normal life and work, and reduces the quality of life. According to reports, yin deficiency and getting angry mainly occur in white-collar workers and college students who are under high pressure from work and school. The incidence of getting angry due to yin deficiency in the age group of 15 to 30 is much higher than that o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12Q1/6851
CPCC12Q1/6851C12Q1/6883C12Q2600/166C12Q2600/178C12Q2563/107C12Q2531/113C12Q2521/107
Inventor 李继承刘昌铭
Owner 李继承
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