6results about How to "Enhance immune regulation function" patented technology

The invention relates to an extraction and culture method of urine-derived stem cells, and belongs to the field of cell biology and regenerative medicine. The method comprises the following steps: coating a culture plate with a gelatin solution; collecting clean midstream urine of healthy people and adding antibiotics; centrifugally separating the cell precipitate; washing with a phosphate buffer solution; re-suspending the precipitate by using a urine stem cell complete culture medium for primary culture; and after the cells grow to a certain fusion degree, carrying out passage amplification. The urine stem cell complete medium comprises a basic medium, fetal calf serum, epidermal growth factors, platelet-derived growth factors and basic fibroblast growth factors. By adopting the method disclosed by the invention, the urine-derived stem cells can be efficiently and stably separated from urine, and the obtained cells have high multiplication capacity, good stemness maintaining capacity, multidirectional differentiation potential and immunoregulation function, and can provide an ideal seed cell source for treating diseases such as diabetic erectile dysfunction.

The application provides a momordica root polysaccharide and a preparation method and application thereof, and belongs to the technical field of natural product development. The preparation method of the momordica root polysaccharide comprises the following steps: drying and crushing momordica roots, and removing fat by ethanol reflux; after water extraction and alcohol precipitation, and removing protein, a momordica root crude polysaccharide is obtained; and the momordica root crude polysaccharide is sequentially separated and purified by DEAE-52 cellulose column and Sephadex LH20 gel column to obtain the momordica root refined polysaccharide. The preparation method has the advantages that the polysaccharide obtained by directly extracting water in the prior art has a narrow molecular weight distribution range and better immune enhancement activity. Experimental results show that the momordica root polysaccharide can stimulate RAW264.7 cells to secrete NO, TNF-alpha and IL-6 factors, enhance the phagocytosis of RAW264.7 cells, and thus enhance the cell immune function, and can be applied to the development of products for improving immunity.

This invention discloses a compound traditional Chinese medicine powder inhaler for respiratory tract infections and its preparation method, relating to the field of pharmaceutical formulation technology. The compound traditional Chinese medicine powder inhaler for respiratory tract infections is composed of polysaccharide composite gel microspheres, forsythia, scutellaria, honeysuckle, and isatis root. The polysaccharide composite gel microspheres are composed of seaweed sulfated polysaccharide, lentinan, calcium chloride, and curcumin. Seaweed sulfated polysaccharide, lentinan, curcumin, and calcium chloride cross-link to form a three-dimensional network spherical structure, improving the particle morphology and flowability of the micropowder. Furthermore, due to its good hygroscopic and moisturizing properties, it competitively reduces the moisture absorption of the micropowder, preventing aggregation and further improving flowability. In addition, the gel microspheres have excellent adhesion, adhering to the infection site after entering the respiratory tract, reducing expulsion losses due to respiration, maintaining an effective drug concentration at the infection site, and improving therapeutic efficacy.

The invention relates to the technical field of bioengineering, in particular to a targeting nanoparticle for treating acute respiratory distress syndrome as well as a preparation method and application of the targeting nanoparticle. Comprising the following steps: (1) combining MP peptide with DSPE-PEG2000-MAL through a maleimide-mercaptan reaction, dialyzing the obtained conjugate, washing, and freeze-drying, so as to obtain a DSPE-PEG2000-MP conjugate; and (2) mixing the conjugate, lipid, a pharmaceutical preparation and a solvent, removing the solvent to obtain a lipid film, hydrating the lipid film, and performing membrane extrusion to obtain the nanoparticles. The invention not only provides a novel efficient and safe ARDS treatment scheme, but also provides an innovative mode for the design of a nano-drug delivery system based on cell mediation. And due to the dual targeting capability, excellent anti-inflammatory efficiency and good biocompatibility, the nano-material has a wide prospect in clinical application.

The application discloses a microwave-probiotics synergistic process for enzymatic Cordyceps militaris protein peptide and application thereof, and belongs to the field of food biotechnology. In view of the problems of low hydrolysis efficiency, poor retention of active ingredients, limited bioavailability and lack of greenness in the preparation of Cordyceps militaris protein peptide, after supercritical CO2 defatting pretreatment, a "complex protease-intermittent microwave-delayed addition of probiotics" synergistic system is constructed, and high-efficiency directional hydrolysis is realized by precise control of parameters; the finished product is obtained through subsequent step-by-step inactivation, 10 kDa ultrafiltration purification and low-temperature vacuum drying; with the synergistic effect of microwave wall breaking and probiotic metabolites (organic acids and peptidases), the degree of protein peptide hydrolysis is greater than or equal to 38%, the proportion of 5-6 peptides is greater than or equal to 90%, the retention rate of cordycepin is greater than or equal to 92%, and the bioavailability is increased by more than 35%; meanwhile, enzyme preparation recycling, residue resourceization and process cost reduction by more than 25% are realized. The protein peptide can be widely applied in the fields of functional food, feed additive and cosmetic raw material, and has economic value and environmental protection benefit.

The application discloses a uniform polysaccharide of Flemingia, a preparation method, a pharmaceutical composition and application thereof, and belongs to the technical field of medicines. The technical problem to be solved is to provide a uniform polysaccharide of Flemingia with high purity and a clear structure, which has macrophage immunoregulation and strong vaccine adjuvant activity, in view of the fact that it is very difficult to prepare the uniform polysaccharide with high purity, a clear structure and batch consistency from natural raw materials on a large scale in the prior art. The technical solution is that the uniform polysaccharide of Flemingia is composed of glucose.