A kind of test kit and application thereof for detecting the serum-specific miRNA of getting angry

Abstract

The present invention relates to the application of miR-122-5p, miR-483-5p and miR-3197 in combination as molecular markers for getting angry in traditional Chinese medicine; it involves the combined use of miR-122-5p, miR-483-5p, miR-3197 and U6 Application in the preparation of traditional Chinese medicine miRNA detection kits; the present invention uses serum hsa-miR-122-5p, hsa-miR-483-5p and hsa-miR-3197 as diagnostic markers, verified by large samples, in practice In people with heat and fire, individual serum hsa-miR-122-5p, hsa-miR-483-5p or hsa-miR-3197 all have significantly low expression, and the kit provided by the invention is used to detect the constitution of excessive heat and fire , through simultaneous relative quantification of serum hsa‑miR‑122‑5p, hsa‑miR‑483‑5p and hsa‑miR‑3197 to identify excess heat and upper body fire constitution, the diagnostic sensitivity is 90.9%, and the specificity is 72.7% , has high accuracy and is higher than the diagnostic properties of a single miRNA. It can replace the traditional TCM diagnostic methods of looking, smelling, asking, and feeling, and combines the diagnosis of TCM syndromes with modern molecular biology to achieve standardization of diagnosis.

Description

technical field [0001] The present invention designs a detection product of miRNA and the application of miR-122-5p, miR-483-5p and miR-3197 as molecular markers for getting angry, and belongs to the field of biotechnology. Background technique [0002] According to the "Group Standards of the Chinese Association of Traditional Chinese Medicine" - Diagnosis and Treatment Guidelines for Getting Fired, getting angry in traditional Chinese medicine refers to the body's head, face, mouth, It is a mild and easily recurring disease with redness, swelling, heat, pain and ulcers on the skin and mucous membranes of the tongue, gums, throat, eyes, nose, etc., and may be accompanied by systemic symptoms. It usually manifests as mouth ulcers, swollen gums, sores on the tongue, red eyes, sore throat, dry mouth, bleeding teeth, epistaxis and other head and face mucosal symptoms, accompanied by symptoms such as red tongue and rapid pulse. With the change of diet structure and the enhancem...

AI Technical Summary

Problems solved by technology

The content of miRNA in serum is much lower than that in tissues, so a very sensitive and specific detection method is required; and because of the short sequence of miRNA primers, primer design is difficult, and no targeted software can be used directly, so both primers and systems need to be screened and tested. optimization process

At present, chip technology is generally used for high-throughput screening of miRNA, which has high cost, limited specificity and sensitivity, and cannot meet the needs of real-time detection of scattered samples; real-time detection of a small number of miRNAs mostly uses Taqman probe method, and some of them have been commercialized Kit products, but the cost and selling price are high

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