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Polyclonal antibody of porcine epidemic diarrhea virus Nsp15 protein and preparation method thereof

A porcine epidemic diarrhea and polyclonal antibody technology, applied in the field of medicine, can solve the problems that hinder the research on the mechanism of Nsp15 antagonizing interferon, lack of Nsp15 protein polyclonal antibody, etc., and achieve good specificity

Pending Publication Date: 2022-07-22
ACAD OF MILITARY SCI PLA CHINA ACAD OF MILITARY MEDICAL SCI INST OF MILITARY VETERINARY MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is currently a lack of polyclonal antibodies against the Nsp15 protein of porcine epidemic diarrhea virus, which hinders the subsequent research on the mechanism of Nsp15 antagonizing interferon

Method used

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  • Polyclonal antibody of porcine epidemic diarrhea virus Nsp15 protein and preparation method thereof
  • Polyclonal antibody of porcine epidemic diarrhea virus Nsp15 protein and preparation method thereof
  • Polyclonal antibody of porcine epidemic diarrhea virus Nsp15 protein and preparation method thereof

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Embodiment Construction

[0023] In order to show the technical solutions, objects and advantages of the present invention more concisely and clearly, the present invention will be further described in detail below with reference to specific embodiments and accompanying drawings. It can be understood that those skilled in the art can refer to the content of this document to appropriately improve the process parameters for implementation. It should be particularly pointed out that all similar substitutions and modifications are obvious to those skilled in the art, and they are deemed to be included in the present invention. The method and application of the present invention have been described through the preferred embodiments, and it is obvious that relevant persons can make changes or appropriate changes and combinations of the methods and applications described herein without departing from the content, spirit and scope of the present invention to achieve and Apply the technology of the present inve...

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Abstract

The invention relates to a polyclonal antibody of porcine epidemic diarrhea virus Nsp15 protein and a preparation method thereof. Prokaryotic expression plasmids of the porcine epidemic diarrhea virus Nsp15 protein are successfully constructed, 37 DEG C is found to be the optimal induction temperature, the high-purity Nsp15 protein is obtained through nickel column purification, the purity is as high as 90%, the protein concentration is 0.69 mg / mL, then the Nsp15 protein polyclonal antibody with high titer is prepared, the titer of the antibody is 2 * 10 < 5 >, a Western Blot test verifies that Nsp15 positive serum specificity is good, and the Nsp15 protein polyclonal antibody can be used for preparing the porcine epidemic diarrhea virus Nsp15 protein polyclonal antibody. The kit can be used for detecting the porcine epidemic diarrhea virus and researching the functional mechanism of Nsp15.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a polyclonal antibody against Nsp15 protein of porcine epidemic diarrhea virus and a preparation method thereof. Background technique [0002] Porcine epidemic diarrhea (PED), discovered in the United Kingdom in 1971, is a severe diarrheal infectious disease of pigs caused by Porcine epidemic diarrhea virus (PEDV) infection. PEDV is generally transmitted by the direct or indirect fecal-oral route. In some cases, airborne transmission may also play a role in PEDV transmission. PEDV is mainly transmitted through diarrheal feces or vomit, but also through clinically or subclinically infected pigs, vehicles transporting pig feces or food, owners, visitors, etc. Other sources of contamination, such as microorganisms in the environment, food, feed, sow milk, etc., may be potential transmission routes of the virus. Newborn piglets are extremely susceptible to PEDV due to low materna...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/10C07K16/06C07K14/165C12N15/70C12N1/21G01N33/569G01N33/68C12R1/19
CPCC07K16/10C07K16/06C07K14/005C12N15/70G01N33/56983G01N33/6854C12N2770/20022C12N2770/20052G01N2333/165G01N2469/10C07K2317/35
Inventor 金宁一李昌李乐天宋利娜鲁会军李霄田明尧郝鹏飞
Owner ACAD OF MILITARY SCI PLA CHINA ACAD OF MILITARY MEDICAL SCI INST OF MILITARY VETERINARY MEDICINE
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