A serum-specific biomarker for diagnosing primary liver cancer and its screening method and application

Abstract

A serum-specific biomarker for the diagnosis of primary liver cancer and its screening method and application, using the Boyden chamber to separate the protrusion and cell body of liver cancer metastatic cells, starting from the subcellular structure, using the improved Boyden chamber method to separate liver cancer metastatic cells For cell bodies and protrusions, the separation results were verified by Western-blot, RNA staining, immunofluorescence and other techniques. Study the differential expression in HCCLM3 liver cancer cell body and protrusion, and screen out microRNAs related to the invasion and metastasis of HCC. Clinical experiments have proved that the change of individual serum hsa-let-7c-3p has a higher diagnostic value for HCC. Sensitivity and specificity. The present invention will provide a new diagnostic method for clinical diagnosis of primary liver cancer, and has certain potential clinical value in assisting the treatment of HCC and monitoring the course of disease, through the ROC curve of let-7c-3p, and let-7c-3p The ROC curves of combined use with CEA, let-7c-3p with AFP, and let-7c-3p with AFP and CEA have improved sensitivity and specificity, and the diagnostic value has been significantly improved.

Description

technical field [0001] The present invention specifically relates to the field of molecular biology technology, in particular to a serum-specific biomarker for diagnosing primary liver cancer, a screening method and application. Background technique [0002] Hepatocellular carcinoma (HCC) is the fifth most common malignant tumor worldwide. Although great progress has been made in the diagnosis and treatment of HCC in recent years, the 5-year survival rate of HCC patients is still quite low due to the frequent clinical recurrence and metastasis of HCC. The occurrence and development of HCC is a typical multi-stage process: first, liver tissue is exposed to external stimuli (hepatitis B virus (HBV) or hepatitis C virus (HCV) infection, aflatoxin B1 intake or alcohol abuse etc.) develop chronic hepatitis or cirrhosis, and then, after going through a series of hyperplasia and dysplasia stages, finally acquire the malignant phenotype of intrahepatic metastasis and distant metast...

AI Technical Summary

Problems solved by technology

[0004] Although some progress has been made in the basic and clinical research of HCC in recent years, the mechanism of HCC metastasis and recurrence has not yet been elucidated.

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