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Method for detecting aortic coarctation markers based on proteomics

A technology of proteomics and aorta, applied in the field of protein detection, to achieve the effect of reducing misdiagnosis rate, high specificity and good sensitivity

Inactive Publication Date: 2018-12-18
NANJING CHILDRENS HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] There are many difficulties in finding biomarkers in serum or plasma

Method used

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  • Method for detecting aortic coarctation markers based on proteomics
  • Method for detecting aortic coarctation markers based on proteomics
  • Method for detecting aortic coarctation markers based on proteomics

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Embodiment Construction

[0071] In order to make the technical solutions of the present invention clearer and clearer to those skilled in the art, the present invention will be further described in detail below in conjunction with the examples and accompanying drawings, but the embodiments of the present invention are not limited thereto.

[0072] Such as figure 1 As shown, the method for proteomics-based detection of aortic coarctation markers provided in this embodiment comprises the following steps:

[0073] Step 1: Sample protein extraction

[0074] Use blood collection tubes to extract iTRAQ experimental serum samples from venous blood, collect the upper serum, and store it at low temperature;

[0075] Step 2: Protein quantification

[0076] The diluted sample and standard were respectively reacted with the protein quantification dye in the dark, and the absorbance values ​​of the standard and sample were measured at the same time, and the protein concentration of each sample was calculated;

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Abstract

The invention discloses a method for detecting aortic coarctation markers based on proteomics, and belongs to the technical field of protein detection. The method comprises the following steps of sample protein extraction, protein quantification, proteolysis, isobaric tags for relative and absolute quantitation (iTRAQ) marking, protein analysis, result detecting and follow-up verification. According to the method for detecting the aortic coarctation markers based on proteomics, a serum specific differential protein of child patients with aortic coarctation is clarified, the protein markers with good sensitivity and high specificity in clinical aortic coarctation laboratory examination are prepared, thus the purpose of decreasing the misdiagnosis rate at the early stage of the disease course of the child patients with aortic coarctation is achieved, the misdiagnosis rate at the early stage of the disease course of the child patients with aortic coarctation can be decreased by combiningconventional echocardiographic examination at the present stage, and import significance is achieved in clinical practical application.

Description

technical field [0001] The invention relates to a method for detecting cortical actin CTTN, in particular to a method for detecting biological markers of aortic coarctation based on blood proteomics, and belongs to the technical field of protein detection. Background technique [0002] Coarctation of the aorta is narrowing of the aorta in the area adjacent to the ductus arteriosus or ligamentum arteriosus. Significant pressure gradients appear when the aortic cross-sectional area is reduced by more than 50%. How to diagnose coarctation of the aorta and carry out early intervention is a clinical problem that needs to be solved urgently. Clinical echocardiography can realize the early diagnosis of congenital heart disease. At present, aortic coarctation is mainly based on echocardiography. However, there are still deficiencies in echocardiography at present, and it cannot be detected 100% of the time. It also has high requirements for sonographers and is highly subjective. ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N30/89
CPCG01N30/89G01N33/6848
Inventor 莫绪明徐诚徐阳束亚琴
Owner NANJING CHILDRENS HOSPITAL
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