Specific antibody of humphead snapper and its prepn process

Abstract

The present invention discloses specific antibody of humphead snapper and its preparation process. The pecific antibody of humphead snapper as high-tech bioengineering product extracted from sea water body includes a serum IgM, a specific yolk antibody and a fish fry antibody with high purity and high specificity. The preparation process includes immunizing sexually matured female humphead snapper with commercial goat IgG, taking parent fish's serum and yolk, promoting propagation, taking fish fry and homogenizing, stepped salting out with saturated ammonium sulfate solution and affinity chromatography to purify the specific antibody. The present invention relates to immunological technology for preventing and treating diseases of aquatic animal, and is significant in raising the disease resistance and survival rate of humphead snapper fry.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a red snapper-specific antibody and a preparation method thereof. Background technique [0002] Within a certain period of time after the larvae of most fish are born or hatched, their own immune system is not yet perfect, they cannot produce antibodies against specific pathogens, and cannot resist the invasion of pathogenic microorganisms in water, resulting in a decline in disease resistance, so that the larvae The survival rate is very low. In the production process of fish, especially seawater fish seedlings in our country, the survival rate of seedlings is very low, such as the survival rate of red snapper seedlings is less than 5%, and the survival rate of grouper seedlings is even lower. Seriously restricting the healthy development of large-scale fish breeding in my country. Traditional methods cannot achieve good results, such as long-term use of antibiotics, which will lea...

AI Technical Summary

Problems solved by technology

The combination of gel filtration and ion exchange chromatography is often used to purify fish antibodies, but the operation is more cumbersome, and the purity of the isolated protein cannot meet the requirements; SPA affinity chromatography technology is also used, and SPA is effective for mammalian IgG. High non-specific binding ability, poor binding ability to fish Ig, resulting in less amount of isolated and purified antibody, and low purity; there are also reports on the purification of antibodies by immunoaffinity chromatography using monoclonal antibodies as ligands, but The preparation process of monoclonal antibodies is more complicated