Method for detection effectiveness of aftosa inactivated vaccines

Abstract

The invention relates to a method for detecting the effectiveness of aftosa inactivated vaccines. The method comprises the following steps that firstly, each dose of each batch of vaccines immunizes 4 to 8 Balb / C series rats, the immunizing dose of the vaccines can be classified into 1 rat / part, one-second rat / part and one-fourth rat / part, blood sampling is performed on the twenty-eighth day, and serum is separated; secondly, serum specific antibodies are detected through an aftosa inhibiting ELISA method, and if antibody inhibiting ELISA titer-log10X is higher than 2.0 and variable coefficient is lower than 12 percent, the antibodies are judged to be qualified. The invention provides a novel method for detecting the effectiveness of aftosa inactivated vaccine, immunizing Balb / C series rats and detecting serum through the method can properly reflect the effective antigen content in the vaccines, and the method replaces the original animal immunization challenge test.

Description

technical field [0001] The invention relates to a method for testing the effectiveness of a foot-and-mouth disease inactivated vaccine. It belongs to the field of biological products for animals. Background technique [0002] Foot-and-mouth disease is still rampant in many developing countries, causing major livestock diseases such as pigs, cattle and sheep, causing huge economic losses. Developed countries have effectively controlled foot-and-mouth disease through culling-based prevention and control measures. For endemic areas and countries, comprehensive prevention and control measures combined with immunization and culling are mainly adopted. Vaccine immunization is the key link, and the quality of vaccines is the top priority. The main indicators for testing the quality control of foot-and-mouth disease inactivated vaccines include endotoxin content, total protein content, and effective antigen content (146S content). There are already detection standards for endotoxin...

AI Technical Summary

Problems solved by technology

Because foot-and-mouth disease is a compulsory immune animal disease in my country, there are very few negative animals for foot-and-mouth disease, resulting in an extreme shortage of animals for efficacy testing; at the same time, there is a biosafety risk in the immune challenge of foot-and-mouth disease, which may cause outbreaks of disease caused by loose virus; there are large individual differences among animals, and the The results have a significant impact and cannot objectively reflect the effective antigen content in the vaccine; in addition, a large number of animal carcasses will be processed to cause environmental pollution; animal experiments cause animal welfare problems. Human and financial resources

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