Method for detection effectiveness of aftosa inactivated vaccines

A technology of inactivated vaccines and testing methods, applied in the field of biological products for animals, can solve problems such as outbreaks of epidemics, large individual differences in animals, and inability to objectively reflect the effective antigen content of vaccines

Inactive Publication Date: 2016-04-20
CHINA INST OF VETERINARY DRUG CONTROL
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Problems solved by technology

Because foot-and-mouth disease is a compulsory immune animal disease in my country, there are very few negative animals for foot-and-mouth disease, resulting in an extreme shortage of animals for efficacy testing; at the same time, there is a biosafety risk in the immune challenge of foot-and-mouth disease, which may cause outbreaks of disease caused by loose

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  • Method for detection effectiveness of aftosa inactivated vaccines
  • Method for detection effectiveness of aftosa inactivated vaccines
  • Method for detection effectiveness of aftosa inactivated vaccines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0013] 1. The relationship between different 146S content of foot-and-mouth disease O-type inactivated antigen and mouse immune serum antibody

[0014] The O-type foot-and-mouth disease inactivated antigen (provided by Jinyu Baoling Biological Pharmaceutical Co., Ltd.) with known 146S content (64 μg / ml) was serially diluted to 0.25 μg / ml, and an equal volume of 206 adjuvant (China Animal Husbandry Co., Ltd. Provided by Lanzhou Biopharmaceutical Factory) prepared vaccine (vaccine antigen content--32μg / ml), each Balb / c was subcutaneously injected with 0.5ml for immunization, each antigen content vaccine was injected into 5 mice, blood was collected for 28 days to separate serum, and liquid phase Serum titer was determined by ELISA kit. The serum titer is represented by -log10X, and the mean value and standard deviation are calculated, and the results are as follows figure 1 shown.

[0015] The antigen content per milliliter of vaccine is correlated with antibody production, an...

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Abstract

The invention relates to a method for detecting the effectiveness of aftosa inactivated vaccines. The method comprises the following steps that firstly, each dose of each batch of vaccines immunizes 4 to 8 Balb/C series rats, the immunizing dose of the vaccines can be classified into 1 rat/part, one-second rat/part and one-fourth rat/part, blood sampling is performed on the twenty-eighth day, and serum is separated; secondly, serum specific antibodies are detected through an aftosa inhibiting ELISA method, and if antibody inhibiting ELISA titer-log10X is higher than 2.0 and variable coefficient is lower than 12 percent, the antibodies are judged to be qualified. The invention provides a novel method for detecting the effectiveness of aftosa inactivated vaccine, immunizing Balb/C series rats and detecting serum through the method can properly reflect the effective antigen content in the vaccines, and the method replaces the original animal immunization challenge test.

Description

technical field [0001] The invention relates to a method for testing the effectiveness of a foot-and-mouth disease inactivated vaccine. It belongs to the field of biological products for animals. Background technique [0002] Foot-and-mouth disease is still rampant in many developing countries, causing major livestock diseases such as pigs, cattle and sheep, causing huge economic losses. Developed countries have effectively controlled foot-and-mouth disease through culling-based prevention and control measures. For endemic areas and countries, comprehensive prevention and control measures combined with immunization and culling are mainly adopted. Vaccine immunization is the key link, and the quality of vaccines is the top priority. The main indicators for testing the quality control of foot-and-mouth disease inactivated vaccines include endotoxin content, total protein content, and effective antigen content (146S content). There are already detection standards for endotoxin...

Claims

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Application Information

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IPC IPC(8): G01N33/53
CPCG01N33/94
Inventor 赵启祖邹兴启朱元源王琴徐璐范学政张乾义丁家波冯忠武冯忠泽印春生郎洪武杨劲松才学鹏宁宜宝
Owner CHINA INST OF VETERINARY DRUG CONTROL
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