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Test strip and kit for detecting human novel coronavirus IgM antibody and preparation method thereof

A coronavirus and test strip technology, applied in the field of immunoassay analysis, can solve the problems of high laboratory cleanliness and operator requirements, false negative nucleic acid test results, high price, etc., and achieve high photochemical stability, Good detection sensitivity and specificity, not easy to decompose

Active Publication Date: 2020-08-14
BIOISLAND LAB +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to improper sampling, improper storage of specimens, use of different types of specimens, and use of reagents from different manufacturers may cause "false negative" nucleic acid test results and missed diagnosis; moreover, nucleic acid detection has high requirements for detection equipment or platforms, and high-sensitivity RT- The PCR instrument is expensive, and has high requirements for the cleanliness of the laboratory and operators; at the same time, nucleic acid detection takes a long time, and it usually takes 4-6 hours to complete an RT-PCR test. However, considering sample transportation, In the case of a large sample backlog, results are usually not available until 24 hours at the earliest

Method used

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  • Test strip and kit for detecting human novel coronavirus IgM antibody and preparation method thereof
  • Test strip and kit for detecting human novel coronavirus IgM antibody and preparation method thereof
  • Test strip and kit for detecting human novel coronavirus IgM antibody and preparation method thereof

Examples

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Effect test

Embodiment 1

[0083] The present embodiment provides a test strip for rapid detection of human novel coronavirus IgM antibody. The test strip includes a PVC bottom plate, a sample pad is connected to the PVC bottom plate, a binding pad, a nitrocellulose membrane (NC membrane) ) and absorbent pads.

[0084] In this embodiment, the components of various solutions used are shown in Table 1 below, and the % in the table represents the mass fraction:

[0085] Table 1

[0086]

[0087] The preparation step of described test strip comprises:

[0088] (1) Preparation of the sample pad: cut the sample pad into a size of 23mm×300mm, soak it in the sample pad treatment solution, take it out after 1 hour, and dry it at room temperature for 16 hours. agent, sealed, and stored at 4°C for later use;

[0089] (2) Preparation of bonding pad: A piece of glass fiber with a size of 200mm×300mm is completely immersed in 50mL of bonding pad treatment solution, and the roller is evenly treated. Take out the...

Embodiment 2～5

[0104] Examples 2-5 provide a plurality of test strips with different concentrations of S1 protein and N protein. The difference from Example 1 is that the working concentrations of the detection line S1 protein and N protein in each embodiment are respectively set as:

Embodiment 2

[0105] Example 2: S1 protein 1.50 mg / mL, N protein 0.50 mg / mL;

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Abstract

The invention provides a test strip and a kit for detecting a human novel coronavirus IgM antibody and a preparation method of the test strip and the kit. A nitrocellulose membrane of the test strip is provided with a detection line and a quality control line; the detection line is coated with novel coronavirus nucleocapsid protein and S1 protein, and the quality control line is coated with a chicken IgY antibody; the combination pad of the test strip is coated with a quantum dot labeled goat anti-human IgM antibody and a quantum dot labeled goat anti-chicken IgY antibody. According to the test strip provided by the invention, novel coronavirus N protein and S1 protein are simultaneously coated on the detection line; the goat anti-human IgM antibody and the goat anti-chicken IgY antibody are labeled by using quantum dots; the human IgM antibody is a serum specific antibody firstly appearing after being infected with novel coronavirus; and the obtained test strip can be used for effectively and accurately detecting patients infected with the novel coronavirus at the early stage, and has important significance for preventing further diffusion of the virus.

Description

technical field [0001] The invention belongs to the technical field of immune detection and analysis, in particular to an immunochromatographic detection test strip, a test kit and a preparation method thereof, in particular to a test strip, a test kit and a preparation method thereof for detecting human novel coronavirus IgM antibody . Background technique [0002] Coronaviruses are a large family of viruses known to cause more serious illnesses such as colds, Middle East Respiratory Syndrome (MERS) and Severe Acute Respiratory Syndrome (SARS). Common signs after a person is infected with a coronavirus include respiratory symptoms, fever, cough, shortness of breath, and dyspnea. In more severe cases, infection can lead to pneumonia, severe acute respiratory syndrome, kidney failure, and even death. [0003] 2019 novel coronavirus (2019-nCoV or SARS-CoV-2) is a kind of coronavirus (Coronavirus), is currently known as the seventh coronavirus that can infect humans, and can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/58G01N33/569G01N33/558G01N33/533G01N33/531
CPCG01N33/6854G01N33/582G01N33/587G01N33/588G01N33/56983G01N33/558G01N33/533G01N33/531G01N2333/165G01N2469/20Y02A50/30
Inventor 张志栋解巧丽齐凯歌陈志龙
Owner BIOISLAND LAB
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