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Food specificity IgE detection kit and preparation method thereof

A detection kit and specific technology, applied in the field of clinical laboratory diagnostics, can solve the problems of inability to combine detection, inability to obtain test results, long operation time, etc., and achieve the effects of saving time for medical treatment, simplifying preparation methods, and simple use

Inactive Publication Date: 2012-11-14
WATSON & CRICK TIANJIN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The problem to be solved by the present invention is to provide a food allergen (specific IgE) detection kit that is simple, fast (10 minutes), and can select a combination of different food-specific IgEs for detection according to the patient's specific conditions, to solve Existing commercial food allergen (specific IgE) detection kits cannot perform "personalized" combined detection according to the specific conditions of patients, and the operation time is long, and the test results cannot be obtained in a short time, etc.

Method used

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  • Food specificity IgE detection kit and preparation method thereof
  • Food specificity IgE detection kit and preparation method thereof
  • Food specificity IgE detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Such as figure 1 , figure 2 Shown, a kind of test reaction plate, its composition comprises sample pad, gold standard pad, NC membrane, water-absorbent material, non-absorbent support sheet; Described sample pad, gold standard pad, NC film and water-absorbent material are pasted on non-absorbent material successively on the support sheet; the sample pad is provided with a sample hole; the gold label pad contains colloidal gold-labeled mouse anti-human IgE monoclonal antibody; the NC membrane is provided with a biotin-labeled bovine serum albumin The detection area and the quality control area coated with goat anti-mouse IgG polyclonal antibody; the detection area and the quality control area are provided with observation ports; the biotin-labeled bovine serum albumin is bound with streptavidin.

Embodiment 2

[0063] A food specific IgE detection kit, its composition comprises:

[0064] (1) the test reaction plate of embodiment 1;

[0065] (2) Eight allergens labeled with biotin - milk allergen, egg allergen, shrimp allergen, crab allergen, peanut allergen, soybean allergen, cashew nut allergen and wheat Allergens, each biotin-labeled allergen is separately packed in a reagent bottle, and used in conjunction with the test reaction plate.

[0066] The preparation method of described test kit comprises the steps:

[0067] 1. Preparation of biotinylated allergen

[0068] (1) Prepare eight kinds of food protein extracts

[0069] A: Egg white extract

[0070] Take about 100ml of egg whites from several raw eggs, add -20°C pre-cooled acetone at a volume ratio of 1:5, soak in 4°C to remove fat for 24 hours, stir twice during the period, change the liquid several times until the acetone is clear. Then centrifuge at 3000rpm for 30min, discard the acetone, weigh the precipitate in a low-...

Embodiment 3

[0125] A method for using the kit described in embodiment 2, comprising the steps of:

[0126] (1) preparation

[0127] Take out the test reaction plate and biotin-labeled allergen, and leave it at room temperature for about 20 minutes;

[0128] (2) test

[0129] Select biotin-labeled allergens (one or several) according to clinical needs, take 1:10 diluted serum (dilute the serum to be tested with 0.85% normal saline) and 200 microliters of biotin-labeled allergens, mix and react After 5 minutes, drop it into the sample hole (S+R) of the test plate, and let it stand for 10 minutes to watch the results.

[0130] (3) Result judgment

[0131] Such as figure 2 As shown, when a single test line (quality control area (C)) appears at the observation port, the result is negative; when two test lines appear, (quality control area (C) and detection area (T)) the result is positive; An invalid test line result appears.

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Abstract

The invention provides a food specificity IgE detection kit and a preparation method thereof. The kit comprises a test reaction plate, and a gold mark cushion and a NC (Numerical Control) film used as a solid phase carrier are arranged on the reaction plate. The kit further comprises various biotin labeled allergens independent from the test reaction plate; the gold mark cushion contains a colloidal gold marked mouse anti-human IgE monoclonal antibody; the NC film is provided with a detection region coated by a biotin labeled bovine serum albumin and a quality control region coated by a goat anti-mouse IgG polyclonal antibody; and the biotin labeled bovine serum albumin is combined with the streptavidin. The kit provided by the invention can be used for 'individualized' combined detection according to specific situation of a patient, is simple to operate and can obtain the detection result within short time.

Description

technical field [0001] The invention belongs to the field of clinical examination and diagnosis, and relates to a food-specific IgE detection kit and a preparation method thereof established by using colloidal gold immunochromatography technology and a biotin-streptavidin system. technical background [0002] 1. The clinical value of specific IgE detection [0003] Allergy, also known as hypersensitivity, refers to an abnormal or pathological immune response that occurs when a body that has been sensitized by an antigen is exposed to the same antigen again, manifested as physiological dysfunction and / or ) tissue damage. Antigens that cause allergic reactions are called allergens. Allergies cause tissue damage or dysfunction and cause clinical manifestations and pathological symptoms. Such diseases are called allergic diseases. Allergic reactions mediated by immunoglobulin E (IgE) involving mast cell nuclei and basophils are called type I hypersensitivity reactions (also k...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577
Inventor 李会强常艳敏彭志军单立新
Owner WATSON & CRICK TIANJIN BIOTECH
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