Method for rapidly detecting sibutramine in weight-reducing health food
A health food, fast technology, applied in material inspection products, measuring devices, biological tests, etc., can solve the problems of being too time-consuming and unsuitable, and achieve the effect of convenient operation, simple steps, and improved sensitivity
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[0022] Attached below Figure 1-5 Embodiments of the present invention will be described in detail.
[0023] 1 Materials and methods
[0024] 1.1 Materials and reagents
[0025] Nitrocellulose membrane (NC membrane), fluorescent silicon ball pad, sample pad, absorbent paper and PVC bottom plate; amino-modified immunofluorescent microspheres (made of polystyrene); sibutramine standard products (sibutramine, N-monodesmethyl sibutramine, N, N-bis demethyl sibutramine, benzyl sibutramine, hamoxibutramine), and other reagents, other reagents are domestic analytical grade . Sibutramine monoclonal antibody and sibutramine antigen were provided by Shenzhen Sanfangyuan Biotechnology Co., Ltd.
[0026] The above-mentioned nitrocellulose membrane (NC membrane), fluorescent silicon ball pad, sample pad, absorbent paper and PVC bottom plate were purchased from Millipore Company in the United States, and the amino-modified fluorescent microspheres were made of pyrogen biological product...
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