25 results about "Protein polymer" patented technology

A protein polymer and a production process therefor. The protein polymer is obtained by means of stimulating MSCs, lysing same, and separating and purifying same. The protein polymer has a strong capability to repair oxidative damage, and thus can reduce the range of cerebral infarction, improve neural functions, inhibit the level of neuroinflammation, and increase the number of surviving neurons.

The invention belongs to the technical field of nutritious food, and particularly relates to a micromolecular donkey-hide gelatin protein polymer which comprises the following components in parts by weight: 40-50 parts of donkey skin, 15-20 parts of deer skin, 10-15 parts of cow leather, 8-12 parts of pigskin, 3-5 parts of sea cucumber, 3-5 parts of fresh oyster, 2-4 parts of snail, 2-4 parts of golden cicada, 5-8 parts of red rock sugar, 4-6 parts of red date and 1-2 parts of fresh ginger. The preparation method comprises the following steps: soaking, cleaning and slicing the raw materials, pre-cooking to remove fishy smell, performing composite enzymolysis, enzyme inactivation and filtration, concentrating and polymerizing, and finally drying and forming. The protein polymer which is appropriate in molecular weight, easy to absorb and comprehensive in nourishing effect is obtained through compatibility of the composite leather raw materials and a precise enzymolysis process, and the absorption efficiency and the comprehensive nourishing effect are improved.

Provided is an application of a protein polymer in the preparation of a drug for treating autism. Research has found that protein polymers derived from mesenchymal stem cells stimulated under stress conditions, such as ultraviolet irradiation, have a certain therapeutic effect on autism, providing a possibility for the treatment of autism.

The present invention provides protein-polymer conjugates and hydrogels containing same, along with methods for generating biocompatible scaffolds formed of hydrogels containing the conjugates and using the scaffolds for tissue regeneration. The present invention also provides improved processes for the preparation of the conjugates, wherein the conjugates of the invention are preferably produced in an environmentally friendly process avoiding polar organic solvents.

The application discloses a kind of targeted dual anti-apoptotic protein polymer micelles and its preparation method and application, to overcome single drug resistance with two kinds of small molecule drugs for inhibiting the expression of anti-apoptotic protein BCL-2 and MCL-1 simultaneously loaded by polymer carrier.VEN and SOR in clinical treatment are obviously hematological toxicity due to the need to use high dose, reduce the overall treatment effect, the application discloses new targeted dual anti-apoptotic protein polymer micelles, effectively solve the problems of large dose, toxicity in the prior art.The experimental results show that TPMs-V / S effectively reduces the dose, toxic side effects of VEN and SOR, effectively improves the curative effect of double drug.

The present disclosure is a lipid nanoparticle including a composition including a protein selected from an antibody and an antibody fragment, a polymer, and a lipid, in which the polymer and the protein have opposite charges at a predetermined pH. The lipid nanoparticle preferably has a protein encapsulated therein. In addition, when the particle diameter of the lipid nanoparticles is measured, the peak top of the particle diameter distribution is preferably at 80 nm to 120 nm.

Imitation leather fabrics comprising a plant protein, plasticizer, and optionally non-proteinaceous polymer and methods of making are disclosed. The fabrics have similar properties to natural leather obtained from animal skin. They are biodegradable, flexible, and stretchable; and can be used as a substitute of natural or synthetic leather.

A protein polymer and a use thereof in the treatment of Alzheimer's disease. The protein polymer is obtained by stimulating MSCs, and then lysing the MSCs and performing separation and purification. The protein polymer has a strong oxidative damage repair ability, can improve Alzheimer's disease-related biomarker abnormalities, and has a significant treatment effect on Alzheimer's disease.

The present invention provides a divalent or trivalent anionic salt cross-linked cryogel, a preparation method therefor, and use thereof, and belongs to the technical field of biomedical materials. The cross-linked cryogel is prepared by cross-linking a polysaccharide and / or a protein polymer cryogel scaffold with a divalent or trivalent anionic salt. The cross-linked cryogel has good mechanical properties, morphological memory properties, hemostatic properties, and the effect of promoting wound healing. The cross-linked cryogel, as a wound dressing, is environmentally friendly and economical, and has characteristics enabling large-scale production, fatigue resistance, breathability, anti-adhesion function, rapid shape memory function after extreme compression, injectability, and exudate absorption capacity, providing a new approach and option for wound repair.

The application provides a chondroitin synthase mutant and application thereof, and relates to the technical field of biology.The chondroitin synthase mutant is obtained by firstly performing deletion on N-terminal and C-terminal amino acid sequences of chondroitin synthase with an amino acid sequence as shown in SEQ ID NO.1 and performing compound mutation on amino acids at positions 200, 279 and 346, introducing the chondroitin synthase mutant into a plasmid to form a recombinant plasmid, introducing the recombinant plasmid into a recombinant bacterium for fermentation culture, and finding that the chondroitin synthase mutant can significantly improve the yield of chondroitin.In addition, the application also overexpresses N-acetylglucosamine epimerase, 6-phosphoglucosamine synthetase, 6-phosphoglucosamine synthetase and uridine diphosphate-glucose dehydrogenase, and constructs the overexpressed enzymes into a protein polymer through CipA protein, so that the synthesis efficiency of chondroitin is improved, and the yield of chondroitin obtained through fermentation in a 5L fermentation tank is about 10.5g / L.

The invention belongs to the technical field of biology, and discloses a protein polymer derived from stem cell lysate obtained through ultrasonic stress culture and a preparation method and application of the protein polymer. The inventor finds out that the protein polymer obtained by cracking, separating and purifying stem cells after ultrasonic stimulation culture has a good cell injury repairing effect, is expected to be used for treating neurodegenerative diseases, cerebral apoplexy, arthritis, post-traumatic recovery, autism, enteritis, depression, pulmonary fibrosis and the like, and particularly has a good application prospect in treatment of neurodegenerative diseases, cerebral apoplexy, arthritis, post-traumatic recovery, autism, enteritis, depression, pulmonary fibrosis and the like. The neurodegenerative diseases include, but are not limited to, Alzheimer's disease (AD), cerebrovascular disease, Parkinson's disease (PD), amyotrophic lateral sclerosis (ALS), different types of spinal cerebellar ataxia (SCA).

The invention belongs to the field of biological medicines, and discloses a protein polymer quality detection method, which comprises at least one of reversed phase HPLC detection, SDS-PAGE analysis, mass spectrum detection, particle size detection, exosome surface marker detection, sterility detection and endotoxin detection. According to the quality detection method of some examples, separation of components in a protein polymer sample can be effectively achieved by optimizing HPLC conditions, a better detection result is achieved, and a quality control standard of the protein polymer is established.

A monolayer, plant protein based imitation leather material and methods of making same. Embodiments of the method comprise 1) creating a mixture of a one or more plant protein powder, a one or more non-proteinaceous polymer or toughening elastomer, a one or more plasticizer, a one or more cross-linking agent, and a one or more curing agent in an; 2) calendering the mixture into a flat, monolayer sheet at a controlled roll temperature and affixing the sheet onto a surface of a carrier fabric and 3) curing the calendered monolayer sheet by applying heat and pressure for a period of time.

Use of a protein polymer in the treatment of amyotrophic lateral sclerosis. The protein polymer is obtained by stimulating and subsequently lysing mesenchymal stem cells, followed by isolation and purification. In SOD1 mutant mouse experiments, the protein polymer can significantly increase the number of motor neurons in the spinal anterior horn, ameliorate muscle atrophy, improve the motor ability of mice, and prolong the survival time of mice. In clinical trials, the protein polymer exhibits the effect of blocking the progression of amyotrophic lateral sclerosis, can reduce the concentration of TDP-43 protein in cerebrospinal fluid, and can increase the concentration of TDP-43 protein in serum.

The application discloses synthesis of a protein polymer self-assembly with multiple environmental stimulus responsiveness and application thereof. Compared with common electrically neutral ELPs, the application finds that the negative electrically modified polymer carrier ELPs are not easy to be taken up by cells and metabolically removed in vivo, have longer drug half-life and bioavailability, and obviously improve pharmacokinetic characteristics; compared with traditional cationic nanoparticles, the negative electrically modified polymer carrier ELPs reduce cytotoxicity caused by positive charges, increase stability, and are easy to realize selective enrichment at an inflammation site, improve drug bioavailability and the like due to in-situ accumulation of part of inflammatory positive charges. The application also adds an MMP9 enzyme recognition site to the hydrophilic end of the ELPs, increases drug release and distribution at the inflammation site, and improves drug efficacy.

A protein polymer and its application in treating spinocerebellar ataxia. The protein polymer is obtained by stimulating MSCs to split, and is separated and purified. The protein polymer has strong oxidative damage repair capacity, can relieve cell stress state by regulating cell gene expression pathway, and promotes cells to restore normal morphology and function.

The invention discloses a modified chitosan magnetic adsorbent, a preparation method thereof and a method for removing macromolecular substances in posterior pituitary injection, and belongs to the technical field of biological medicine purification. The method for removing the macromolecular substances in the posterior pituitary injection comprises the following steps: heating a glacial acetic acid solution, adding posterior pituitary powder and chitosan, preserving heat, stirring, filtering residues, adjusting the pH value, heating, adding a modified chitosan magnetic adsorbent, stirring, cooling, filtering to remove the adsorbent, and filtering through a multi-stage microporous filter membrane to obtain a finished product of the posterior pituitary injection. According to the method, impurities such as high-molecular protein polymers and endotoxin in the posterior pituitary injection can be efficiently removed in a targeted mode, the removal rate reaches 100% or above, meanwhile, active ingredients are reserved to the maximum extent, the process is simplified, the efficiency and controllability are improved, the preparation purity and medication safety are remarkably improved, and the method is suitable for purification of polypeptide biological preparations and has wide industrial application value.

The invention discloses a photodynamic bacteriostasis-ultraviolet barrier composite film as well as a preparation method and application thereof, and belongs to the technical field of film materials. The composite film comprises a film substrate and a modified photoresponse material coated in the film substrate, the preparation raw materials of the membrane substrate comprise polysaccharide macromolecules, natural protein macromolecules and synthetic macromolecules; the modified photoresponse material is HOFs containing a metal center. Natural polysaccharide macromolecules, natural protein macromolecules and synthetic macromolecules are adopted as a film forming substrate, the raw materials are wide in source, non-toxic, harmless and biodegradable, and by means of the good film forming property and flexibility of the synthetic macromolecules and the anti-oxidation characteristic of the natural protein macromolecules, the natural polysaccharide macromolecules, the natural protein macromolecules and the synthetic macromolecules serve as the film forming substrate. Natural polysaccharide macromolecules, natural protein macromolecules and synthetic macromolecules are mixed to prepare a mixed film-forming system which has excellent antibacterial and fresh-keeping effects and can solve the problems that a single natural polysaccharide macromolecule solution is poor in mechanical property, insufficient in antioxidant activity and the like.

The invention belongs to the field of quality control of biological products, and discloses an activity detection method of a protein polymer. The inventor finds that NFL can be detected in a cultured culture medium after oxidative damage of nerve cells, and the NFL is related to the oxidative damage degree of the nerve cells. By detecting the amount of the NFL in the culture medium, the nerve injury repairing capacity can be better represented, the NFL directly represents the nerve injury degree, the nerve cell oxidative injury degree can be conveniently, rapidly and sensitively determined, and the biological activity of the protein polymer to the nerve cells can be better evaluated.

The present invention relates to the field of biopharmaceuticals, and discloses a quality detection method for a protein polymer, comprising at least one of reverse-phase HPLC detection and SDS-PAGE analysis, mass spectrometry detection, particle size detection, exosome surface marker detection, sterile detection, and endotoxin detection. In some embodiments of the present invention, the quality detection method effectively achieves separation of components in a protein polymer sample by optimizing HPLC conditions, thereby obtaining better detection results, and establishing a quality control standard for the protein polymer.

To provide pramlintide analogs that retain amylin agonist activity and have advantages such as extended half-life and reduced tendency to fibrillate.SOLUTION: A polypeptide having a specific sequence, which is a pramlintide analogue conjugated to a half-life extending moiety, such as an albumin binding moiety. The disclosed peptides can be formulated in or chemically conjugated to, for example, proteins, polymeric drug carriers or pre-drug delivery systems, which enhance the chemical and or physical stability and or circulatory exposure of the therapeutic moiety.SELECTED DRAWING: None

Provided is an MSC culture process for improving the yield of a protein polymer. The culture process comprises resuscitating and seeding mesenchymal stem cells, transferring same into a culture vessel with a microcarrier for incubation by rotation or shaking, and aseptically transferring same to a culture bag after culturing; subjecting the culture bag to irradiation stimulation; and after completing the stimulation, lysing the mesenchymal stem cells, and separating and purifying same to obtain a protein polymer. The MSC culture process allows for the acquisition of more MSCs, which can be purified to obtain more protein polymers.

The invention discloses a purification method of a high-purity astrocyte exosome, and relates to the technical field of exosome purification. Comprising the following steps: S1, culturing astrocytes and collecting supernatant; s2, pretreatment: removing cells and large-size impurities; s3, carrying out gradient filtration, and preliminarily enriching exosomes; s4, carrying out improved density gradient centrifugation and deep purification; s5, carrying out affinity purification, and removing residual impurities; and S6, preserving the exosome. The purification purity is higher, through combination of three-step purification, the purity of the exosome is far higher than that of the exosome obtained through a traditional ultracentrifugation method and a kit method, and no impurity residues such as cell debris and protein polymers exist; the recovery rate is higher, the overall recovery rate is remarkably improved compared with that of a traditional ultracentrifugation method, and exosome loss in the subsequent centrifugation process is reduced due to preliminary enrichment of gradient filtration; through low-temperature operation and mild elution conditions in the whole process, the integrity rate of an exosome membrane structure and the retention rate of functional protein and miRNA are all superior to those in the prior art.

A nanofiber comprising a cross-linkable protein polymer and a transglutaminase. A method for producing a nanofiber, the method comprising: combining a first solution comprising a cross-linkable protein polymer and a second solution comprising a transglutaminase to produce a liquid electro spinning mixture (LEM) characterized by an environment that is unsuitable for the transglutaminase to be enzymatically active on the cross-linkable protein polymer; and electro spinning the LEM to form a nanofiber comprising the TGase and the cross-linkable protein polymer.

Provided are a protein polymer and the use thereof in the treatment of spinocerebellar ataxia disease. The protein polymer is obtained by means of stimulating MSCs and then lysing same, followed by isolation and purification. The protein polymer has strong oxidative damage repair capability, and can relieve a cellular stress status by means of modifying a cellular gene expression pathway, thereby promoting the restoration of normal cellular morphology and function.