49 results about "Protein polymer" patented technology

The invention relates to a protein-based phenylboronic acid ester dynamic crosslinking hydrogel material as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving a component A, namely a phenylboronic acid modified protein polymer derivative, in a biocompatible medium to obtain a solution A; dissolving a component B, namely a macromolecular derivative containing a vicinal diol group, into a biocompatible medium to obtain a solution B; and mixing the solution A and the solution B with a certain concentration for a certain time to obtain the protein-based phenylboronic acid ester dynamic crosslinking hydrogel material. The boric acid ester dynamic cross-linking bond belongs to reversible chemical cross-linking reaction, and the prepared protein-based hydrogel material has enough mechanical strength and cheap operability. The protein-based hydrogel material disclosed by the invention can provide the effects of cell adhesion, proliferation and differentiation promotion, tissue regeneration and the like, can load and slowly release beneficial components for promoting tissue repair, is an ideal tissue engineering scaffold material, and can be applied to defect regeneration and repair of soft tissues, cartilages, bones and the like.

The invention relates to a method for detecting and / or purifying a protein polymer and application thereof. The method comprises a step of detecting the protein polymer based on HPLC-SEC. The chromatographic conditions are as follows: a chromatographic column is a molecular size exclusion high performance liquid chromatographic column; and a PB buffer solution with the concentration of 50-200mM is used as a mobile phase. The method disclosed by the invention is good in separation degree, simple to operate, short in peak appearance time and short in detection time, and has good method reproducibility and stability.

The invention belongs to the field of biological medicines, and discloses an application of a protein polymer in preparation of a medicine for treating Alzheimer's disease, a production process of the protein polymer comprises the following steps: S1) using ultraviolet irradiation to stimulate mesenchymal stem cells and culturing; and S2) splitting the mesenchymal stem cells, separating and purifying to obtain the protein polymer. According to the present invention, the mesenchymal stem cells are stimulated to express the protein with the nerve repair function through the ultraviolet irradiation stress mode, and the protein polymer is obtained through further separation and purification, such that the protein polymer can be adapted to diversified application modes, can improve the abnormal conditions of the Alzheimer's disease related biomarkers, and can provide the significant treatment effect on the Alzheimer's disease.

The invention belongs to the field of biological medicines, and discloses an application of a protein polymer in preparation of a medicine for treating spinal cerebellar ataxia disease, a production process of the protein polymer comprises the following steps: S1) using ultraviolet irradiation to stimulate mesenchymal stem cells and culturing; and S2) splitting the mesenchymal stem cells, separating and purifying to obtain the protein polymer. An ultraviolet irradiation stress mode is adopted, mesenchymal stem cells are stimulated to express protein with a neural repair function, the protein polymer is obtained through further separation and purification, the cell gene expression pathway is regulated, the cell stress state is relieved, the cells are promoted to recover normal forms and functions, meanwhile, the preparation mode is relatively simple, and the preparation method is suitable for large-scale production. And quality and yield monitoring is facilitated, and the method can be adapted to diversified application modes.

The present invention provides protein-polymer conjugates, and methods for generating biocompatible scaffolds formed of hydrogels comprising the conjugates and use of the scaffolds for tissue regeneration. The present invention provides improved processes for the preparation of the conjugates, wherein the conjugates of the invention are preferably produced in an environmentally friendly process avoiding polar organic solvents.

A protein polymer and a production process therefor. The protein polymer is obtained by means of stimulating MSCs, lysing same, and separating and purifying same. The protein polymer has a strong capability to repair oxidative damage, and thus can reduce the range of cerebral infarction, improve neural functions, inhibit the level of neuroinflammation, and increase the number of surviving neurons.

The invention belongs to the technical field of nutritious food, and particularly relates to a micromolecular donkey-hide gelatin protein polymer which comprises the following components in parts by weight: 40-50 parts of donkey skin, 15-20 parts of deer skin, 10-15 parts of cow leather, 8-12 parts of pigskin, 3-5 parts of sea cucumber, 3-5 parts of fresh oyster, 2-4 parts of snail, 2-4 parts of golden cicada, 5-8 parts of red rock sugar, 4-6 parts of red date and 1-2 parts of fresh ginger. The preparation method comprises the following steps: soaking, cleaning and slicing the raw materials, pre-cooking to remove fishy smell, performing composite enzymolysis, enzyme inactivation and filtration, concentrating and polymerizing, and finally drying and forming. The protein polymer which is appropriate in molecular weight, easy to absorb and comprehensive in nourishing effect is obtained through compatibility of the composite leather raw materials and a precise enzymolysis process, and the absorption efficiency and the comprehensive nourishing effect are improved.

A monolayer, plant protein based imitation leather material and methods of making same. Embodiments of the method comprise 1) creating a mixture of a one or more plant protein powder, a one or more non-proteinaceous polymer or toughening elastomer, a one or more plasticizer, a one or more cross-linking agent, and a one or more curing agent in an; 2) calendering the mixture into a flat, monolayer sheet at a controlled roll temperature and affixing the sheet onto a surface of a carrier fabric and 3) curing the calendered monolayer sheet by applying heat and pressure for a period of time.

The present invention relates to multivalent protein-polymer conjugates, compositions, and methods for treating uveitis, such as chronic non-infectious uveitis.

Provided is an application of a protein polymer in the preparation of a drug for treating autism. Research has found that protein polymers derived from mesenchymal stem cells stimulated under stress conditions, such as ultraviolet irradiation, have a certain therapeutic effect on autism, providing a possibility for the treatment of autism.

The present invention provides protein-polymer conjugates and hydrogels containing same, along with methods for generating biocompatible scaffolds formed of hydrogels containing the conjugates and using the scaffolds for tissue regeneration. The present invention also provides improved processes for the preparation of the conjugates, wherein the conjugates of the invention are preferably produced in an environmentally friendly process avoiding polar organic solvents.

The application discloses a kind of targeted dual anti-apoptotic protein polymer micelles and its preparation method and application, to overcome single drug resistance with two kinds of small molecule drugs for inhibiting the expression of anti-apoptotic protein BCL-2 and MCL-1 simultaneously loaded by polymer carrier.VEN and SOR in clinical treatment are obviously hematological toxicity due to the need to use high dose, reduce the overall treatment effect, the application discloses new targeted dual anti-apoptotic protein polymer micelles, effectively solve the problems of large dose, toxicity in the prior art.The experimental results show that TPMs-V / S effectively reduces the dose, toxic side effects of VEN and SOR, effectively improves the curative effect of double drug.

The present disclosure is a lipid nanoparticle including a composition including a protein selected from an antibody and an antibody fragment, a polymer, and a lipid, in which the polymer and the protein have opposite charges at a predetermined pH. The lipid nanoparticle preferably has a protein encapsulated therein. In addition, when the particle diameter of the lipid nanoparticles is measured, the peak top of the particle diameter distribution is preferably at 80 nm to 120 nm.

The invention provides a preparation combination formula of pegylated phenylalanine lyase. According to the method, the physical change and chemical change of the pegylated phenylalanine lyase caused by the influence of salt, oscillation, pH and temperature are solved, particularly the technical problems of high temperature, oscillation and freeze-thaw stability can be solved, and the stability and activity effect of the pegylated phenylalanine lyase protein polymer can be kept for a long time.

The present invention belongs to the field of biotechnology and discloses a protein polymer and its production process. The protein polymer is obtained by stimulating MSCs, followed by lysis, isolation, and purification. The protein polymer has strong oxidative damage repair capabilities, can reduce the extent of cerebral infarction, improve neurological function, inhibit neuroinflammation, and increase the number of surviving neurons.

Imitation leather fabrics comprising a plant protein, plasticizer, and optionally non-proteinaceous polymer and methods of making are disclosed. The fabrics have similar properties to natural leather obtained from animal skin. They are biodegradable, flexible, and stretchable; and can be used as a substitute of natural or synthetic leather.

The application belongs to the field of biological drugs, and discloses a quality detection method of protein polymer, which comprises at least one of reverse phase HPLC detection, SDS-PAGE analysis, mass spectrometry detection, particle size detection, exosome surface marker detection, sterility detection and endotoxin detection. The quality detection method of some examples of the application can effectively realize the separation of components in the protein polymer sample by optimizing the HPLC condition, achieve better detection results, and establish the quality control standard of the protein polymer.

A protein polymer and a use thereof in the treatment of Alzheimer's disease. The protein polymer is obtained by stimulating MSCs, and then lysing the MSCs and performing separation and purification. The protein polymer has a strong oxidative damage repair ability, can improve Alzheimer's disease-related biomarker abnormalities, and has a significant treatment effect on Alzheimer's disease.

The present invention relates generally to an isolated polypeptide from Hylaeus nubilosus, a proteinaceous polymer comprising the polypeptide or a portion thereof, a composition comprising the polypeptide and / or polymer or a portion thereof, and a method of recombinantly producing the polypeptide and / or polymer or a portion thereof.

The present invention provides a divalent or trivalent anionic salt cross-linked cryogel, a preparation method therefor, and use thereof, and belongs to the technical field of biomedical materials. The cross-linked cryogel is prepared by cross-linking a polysaccharide and / or a protein polymer cryogel scaffold with a divalent or trivalent anionic salt. The cross-linked cryogel has good mechanical properties, morphological memory properties, hemostatic properties, and the effect of promoting wound healing. The cross-linked cryogel, as a wound dressing, is environmentally friendly and economical, and has characteristics enabling large-scale production, fatigue resistance, breathability, anti-adhesion function, rapid shape memory function after extreme compression, injectability, and exudate absorption capacity, providing a new approach and option for wound repair.

The application provides a chondroitin synthase mutant and application thereof, and relates to the technical field of biology.The chondroitin synthase mutant is obtained by firstly performing deletion on N-terminal and C-terminal amino acid sequences of chondroitin synthase with an amino acid sequence as shown in SEQ ID NO.1 and performing compound mutation on amino acids at positions 200, 279 and 346, introducing the chondroitin synthase mutant into a plasmid to form a recombinant plasmid, introducing the recombinant plasmid into a recombinant bacterium for fermentation culture, and finding that the chondroitin synthase mutant can significantly improve the yield of chondroitin.In addition, the application also overexpresses N-acetylglucosamine epimerase, 6-phosphoglucosamine synthetase, 6-phosphoglucosamine synthetase and uridine diphosphate-glucose dehydrogenase, and constructs the overexpressed enzymes into a protein polymer through CipA protein, so that the synthesis efficiency of chondroitin is improved, and the yield of chondroitin obtained through fermentation in a 5L fermentation tank is about 10.5g / L.

The invention belongs to the technical field of biology, and discloses a protein polymer derived from stem cell lysate obtained through ultrasonic stress culture and a preparation method and application of the protein polymer. The inventor finds out that the protein polymer obtained by cracking, separating and purifying stem cells after ultrasonic stimulation culture has a good cell injury repairing effect, is expected to be used for treating neurodegenerative diseases, cerebral apoplexy, arthritis, post-traumatic recovery, autism, enteritis, depression, pulmonary fibrosis and the like, and particularly has a good application prospect in treatment of neurodegenerative diseases, cerebral apoplexy, arthritis, post-traumatic recovery, autism, enteritis, depression, pulmonary fibrosis and the like. The neurodegenerative diseases include, but are not limited to, Alzheimer's disease (AD), cerebrovascular disease, Parkinson's disease (PD), amyotrophic lateral sclerosis (ALS), different types of spinal cerebellar ataxia (SCA).

The invention belongs to the field of biological medicines, and discloses a protein polymer quality detection method, which comprises at least one of reversed phase HPLC detection, SDS-PAGE analysis, mass spectrum detection, particle size detection, exosome surface marker detection, sterility detection and endotoxin detection. According to the quality detection method of some examples, separation of components in a protein polymer sample can be effectively achieved by optimizing HPLC conditions, a better detection result is achieved, and a quality control standard of the protein polymer is established.

A monolayer, plant protein based imitation leather material and methods of making same. Embodiments of the method comprise 1) creating a mixture of a one or more plant protein powder, a one or more non-proteinaceous polymer or toughening elastomer, a one or more plasticizer, a one or more cross-linking agent, and a one or more curing agent in an; 2) calendering the mixture into a flat, monolayer sheet at a controlled roll temperature and affixing the sheet onto a surface of a carrier fabric and 3) curing the calendered monolayer sheet by applying heat and pressure for a period of time.

Use of a protein polymer in the treatment of amyotrophic lateral sclerosis. The protein polymer is obtained by stimulating and subsequently lysing mesenchymal stem cells, followed by isolation and purification. In SOD1 mutant mouse experiments, the protein polymer can significantly increase the number of motor neurons in the spinal anterior horn, ameliorate muscle atrophy, improve the motor ability of mice, and prolong the survival time of mice. In clinical trials, the protein polymer exhibits the effect of blocking the progression of amyotrophic lateral sclerosis, can reduce the concentration of TDP-43 protein in cerebrospinal fluid, and can increase the concentration of TDP-43 protein in serum.

The present invention relates to a nano-in micro formulation, capable of enhancing the oral bioavailability of drugs. Said formulation is made of a drug loaded composite nanoparticles made of protein-polymer, which is embedded within a microsystem (nano-in micro) made of multiple layers, each layer serving specific function such as enteric protection, muco-adhesion, muco-penetration, release modification, efflux inhibition, all functions collectively resulting in the enhanced bioavailability of loaded drug by at least two-fold when compared with control free drug. The present invention also discloses the process for conceiving said nano-in micro formulation and describes the use of said nano-in micro formulation in the treatment of cancer and other diseases.

The application discloses synthesis of a protein polymer self-assembly with multiple environmental stimulus responsiveness and application thereof. Compared with common electrically neutral ELPs, the application finds that the negative electrically modified polymer carrier ELPs are not easy to be taken up by cells and metabolically removed in vivo, have longer drug half-life and bioavailability, and obviously improve pharmacokinetic characteristics; compared with traditional cationic nanoparticles, the negative electrically modified polymer carrier ELPs reduce cytotoxicity caused by positive charges, increase stability, and are easy to realize selective enrichment at an inflammation site, improve drug bioavailability and the like due to in-situ accumulation of part of inflammatory positive charges. The application also adds an MMP9 enzyme recognition site to the hydrophilic end of the ELPs, increases drug release and distribution at the inflammation site, and improves drug efficacy.

A protein polymer and its application in treating spinocerebellar ataxia. The protein polymer is obtained by stimulating MSCs to split, and is separated and purified. The protein polymer has strong oxidative damage repair capacity, can relieve cell stress state by regulating cell gene expression pathway, and promotes cells to restore normal morphology and function.

Provided are a DNA analysis kit for analyzing DNA through scanning electron microscopy (SEM), a DNA-protein-polymer complex, a composition including the DNA-protein-polymer complex, and a DNA analysis method using the DNA-protein-polymer complex. The DNA-protein-polymer complex includes: a DNA molecule; a DNA-binding protein including a peptide, the peptide having an amino acid sequence capable of binding to the DNA molecule and having at least one functional group in at least one of both terminuses thereof; and a polymer, an anhydride thereof or a salt thereof, capable of binding to the DNA-binding protein by intermolecular electrostatic attraction. The DNA-protein-polymer complex is metal-free and enables high-resolution observation and analysis of DNA molecules having various shapes and lengths at almost real-time speed through SEM.

The invention discloses a modified chitosan magnetic adsorbent, a preparation method thereof and a method for removing macromolecular substances in posterior pituitary injection, and belongs to the technical field of biological medicine purification. The method for removing the macromolecular substances in the posterior pituitary injection comprises the following steps: heating a glacial acetic acid solution, adding posterior pituitary powder and chitosan, preserving heat, stirring, filtering residues, adjusting the pH value, heating, adding a modified chitosan magnetic adsorbent, stirring, cooling, filtering to remove the adsorbent, and filtering through a multi-stage microporous filter membrane to obtain a finished product of the posterior pituitary injection. According to the method, impurities such as high-molecular protein polymers and endotoxin in the posterior pituitary injection can be efficiently removed in a targeted mode, the removal rate reaches 100% or above, meanwhile, active ingredients are reserved to the maximum extent, the process is simplified, the efficiency and controllability are improved, the preparation purity and medication safety are remarkably improved, and the method is suitable for purification of polypeptide biological preparations and has wide industrial application value.