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IgE antigenicity sequence and preparation of branched multiple antigenic peptides

An antigenic and antigenic peptide technology, applied in the field of preparation of IgE antigenic sequences and branched multimeric antigenic peptides, can solve problems such as weak immunogenicity, and achieve the effect of overcoming weak immunogenicity and strong antibody specificity

Inactive Publication Date: 2009-08-12
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

The branched multimeric antigenic peptide obtained in the present invention can solve the disadvantage of weak immunogenicity of the linear peptide of the antigenic epitope alone, and can effectively induce the antibody response to the Cε3 domain of the human IgE heavy chain in the immunized animal

Method used

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  • IgE antigenicity sequence and preparation of branched multiple antigenic peptides

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Experimental program
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Effect test

Embodiment

[0020] Step 1, using the peptide sequence shown in SEQ ID NO.1, using the Fmoc solid-phase peptide synthesis method to synthesize an eight-branched polyantigen peptide with lysine as a scaffold, and collecting and lyophilizing it for later use

[0021]Flexibility, antigenicity, secondary structure, hydrophilic region, protein surface accessibility index of the human IgE immunoglobulin heavy chain Cε3 region using the Kolaskar-Tongaonkar antigenicity analysis method and the Protean module in the DNA Star software package The epitope parameters and epitope index were analyzed comprehensively. According to the software analysis results, in the Cε3 amino acid segment of the human IgE immunoglobulin heavy chain: the antigenic index ≥ 1.0204, the hydrophilic index ≥ 0, the surface possibility index of amino acids ≥ 1, and there is a flexible structure in or near it. We selected the amino acid sequence shown in SEQ ID NO.1 near the carboxyl terminal from the amino acid segment meetin...

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Abstract

The invention relates to IgE antigenicity sequence in the immunological technical field and a preparation method for branch multiple antigenic peptides; wherein, the IgE antigenicity sequence has amino acid sequence shown in the SEQ ID NO.I; the preparation method for the branch multiple antigenic peptides comprises the following steps: the peptide sequence shown in the SEQ ID NO.I is used, a Fmoc solid phase peptide synthesis method is used for synthesizing eight branch multiple antigen peptide taking lysine as a bracket; after being collected and freezing out, the eight branch multiple antigen peptide is reserved; a HPLC method purifies the branch multiple antigenic peptides obtained in the step one. The branch multiple antigenic peptides prepared by the method can solve weak immunogenicity when the antigen epitope linear peptide is separately used and effectively gives rises to antibody response aiming at human IgE heavy chain C epsilon3 structure in immune animals.

Description

technical field [0001] The invention relates to a method for preparing an antigenic sequence and a branched multimeric antigenic peptide in the field of immunization technology, in particular to a method for preparing an IgE antigenic sequence and a branched multimeric antigenic peptide. Background technique [0002] Immunoglobulin IgE plays a key mediating role in the development of asthma, seasonal allergic rhinitis and other allergic diseases. When allergens enter the body for the first time, allergen-specific B cells are selectively induced to produce IgE antibody responses, and free IgE in serum can interact with mast cells and basophils through its Fc fragment without binding antigens The binding of high-affinity FcεRI receptor fragments on the cell surface puts the body in a sensitized state. After the body is exposed to the allergen again, the multivalent allergen binds to two or more adjacent IgE antibodies on the surface of the sensitized cell, cross-linking the F...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C07K14/00C07K1/04
Inventor 杨浩崔大祥
Owner SHANGHAI JIAO TONG UNIV
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