Method of anaphylactogen screening

Abstract

The invention is an allergen screening method, comprising the steps of: a. taking removable ELISA plate stripe as allergen screening carrier, and coating common allergens in ELISA plate holes; b. adding human serum into the ELISA plate stripe to make IgE antibodies in the serum specifically bind with the allergens, and then adding in biotin-labeled anti-human IgE to specifically form combination; c. successively adding in streptavidin-Horse Radish Peroxidase (streptavidin-HRP) and using biotin-avidin specific binding to form compound; d. finally adding in allergen color developing agent, and under the action of HRP, making the color developing agent change color, and determining the concentration of IgE antibodies in the sample according to the shade of color. And it is simple to operate and has accurate result and low cost.

Description

technical field [0001] The invention relates to a method for detecting allergens that cause allergic reactions in humans, in particular to an allergen screening method for in vitro detection of allergens. Background technique [0002] An allergic reaction, also known as an allergic reaction, is an abnormal reaction of the body to one or more substances that are harmless to most people. The main cause is that patients with allergic diseases produce too much of a special antibody called immunoglobulin E (IgE). It can react with allergens (allergens) in the environment, stimulate the body to produce and release certain excessive chemical substances, and then produce various symptoms. [0003] Allergic disease, also known as allergic disease, refers to the tissue damage or physiological function caused by the stimulation of the body by antigenic substances (also known as allergens or allergens), such as pollen, dust, food, drugs, parasites, etc. Disorder, which is an abnormal ...

AI Technical Summary

Problems solved by technology

The MAST CLA allergen detection system of American HITACHI Chemical Diagnostics Company adopts chemiluminescence technology, the German PCA allergen detection system adopts solid-phase enzyme spot technology, the American Dexall(R) allergen rapid detection box adopts chromatography colloidal gold technology, and the German MEDIWISS "sensitivity screen" The quantitative allergen detection system uses immunoblotting technology to detect patient serum in vitro. The kits of these companies basically use cellulose membrane as a carrier, and specific allergen analysis instruments are used for machine reading. The instruments are relatively expensive, and the price of reagents per person same high

[0011] There is also a kind of allergen detection kit using test tube reaction plate in China. The representative products are IVT products and QD allergen detection reagent packaged by Zhejiang Shangyu Quick. Machine-readable scale, only for qualitative judgment

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