Method of anaphylactogen screening

An allergen and screening technology, which is applied in the field of allergen screening, can solve the problems of high price per serving of reagents and expensive instruments, and achieve the effects of easy promotion and use, simple operation and accurate results

Inactive Publication Date: 2006-11-29
深圳市博卡生物技术有限公司
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Problems solved by technology

The MAST CLA allergen detection system of American HITACHI Chemical Diagnostics Company adopts chemiluminescence technology, the German PCA allergen detection system adopts solid-phase enzyme spot technology, the American Dexall(R) allergen rapid detection box adopts chromatography colloidal gold technology, and the German MEDIWISS "sensitivity screen" The quantitative allergen detection system uses immunoblotting technology to detect patient serum in vitro. The kits of these

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  • Method of anaphylactogen screening

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Embodiment Construction

[0028] The following examples are further explanations and illustrations of the present invention, and do not constitute any limitation to the present invention.

[0029] figure 1 The flow chart of the allergen screening method of the present invention is shown, as shown in the figure, the method includes the following steps:

[0030] a. The enzyme label strip is used as the carrier for screening allergens. The enzyme label strip is detachable, and it is movably placed in the kit, with enzyme label holes on it. ELISA strips can be conventional products in the art. Coat the common allergens in the wells of the microtiter plate before testing;

[0031] b. Add the human serum of the test subject to the well of the microtiter plate, so that the IgE antibody in the serum can specifically bind to the allergen, and then add biotin-labeled anti-human IgE to specifically form the allergen-IgE-biological Anti-human IgE conjugates;

[0032] c, then add bioactive material avidin-horse...

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Abstract

The invention is an allergen screening method, comprising the steps of: a. taking removable ELISA plate stripe as allergen screening carrier, and coating common allergens in ELISA plate holes; b. adding human serum into the ELISA plate stripe to make IgE antibodies in the serum specifically bind with the allergens, and then adding in biotin-labeled anti-human IgE to specifically form combination; c. successively adding in streptavidin-Horse Radish Peroxidase (streptavidin-HRP) and using biotin-avidin specific binding to form compound; d. finally adding in allergen color developing agent, and under the action of HRP, making the color developing agent change color, and determining the concentration of IgE antibodies in the sample according to the shade of color. And it is simple to operate and has accurate result and low cost.

Description

technical field [0001] The invention relates to a method for detecting allergens that cause allergic reactions in humans, in particular to an allergen screening method for in vitro detection of allergens. Background technique [0002] An allergic reaction, also known as an allergic reaction, is an abnormal reaction of the body to one or more substances that are harmless to most people. The main cause is that patients with allergic diseases produce too much of a special antibody called immunoglobulin E (IgE). It can react with allergens (allergens) in the environment, stimulate the body to produce and release certain excessive chemical substances, and then produce various symptoms. [0003] Allergic disease, also known as allergic disease, refers to the tissue damage or physiological function caused by the stimulation of the body by antigenic substances (also known as allergens or allergens), such as pollen, dust, food, drugs, parasites, etc. Disorder, which is an abnormal ...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N21/78
Inventor 雷均平何素华邱于斌赵振军薛晓英
Owner 深圳市博卡生物技术有限公司
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