The invention discloses a liquid phase chip for detecting an allergen specific antibody. The liquid phase chip mainly comprises (1) a plurality of common allergen probes, comprising 12 fluorescence coded microspheres respectively coated with mite, cockroach, ragweed, felon herb, dog hairs, cat hairs, soybean, peanut, milk, egg, shrimp and crab allergen extracts; (2) a biotin labeling detection antibody; (3) a streptavidin phycoerythrin; (4) a total IgE (immunoglobulin E) probe used when semiquantitative or quantitative detection is carried out on the allergen specific IgE antibody, namely, a fluorescence coded microsphere coated with a rat anti-human IgE monoclonal antibody. The invention also discloses a preparation method of the liquid phase chip probe. The liquid phase chip disclosed by the invention is mainly used for detecting the allergen specific IgE antibody in a serum sample, also for detecting an allergen specific IgG4 (Intravenous Gamma Globulin 4) antibody and a total IgE antibody, and has the advantages of few used samples, parallel detection of a plurality of indexes, high flux, high sensitivity and the like.