Test strip for specific milk allergen ige screening and preparation method thereof

A technology of test strips and allergens, which is applied in the field of test strips and its preparation for IgE screening of specific milk allergens, can solve the problems of long test time, poor sensitivity and precision, cumbersome operation, etc. Simple, accurate and low-cost effects

Active Publication Date: 2016-01-13
BIOSINO BIO TECH & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are disadvantages of cumbersome operation, poor sensitivity and precision, and long test time

Method used

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Examples

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Embodiment Construction

[0027] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available products.

[0028] Example Test strips for IgE screening of specific milk allergens and preparation methods thereof

[0029] Preparation of test strips for IgE screening of specific milk allergens includes preparation of antigen solution to be labeled, preparation of colloidal gold solution to be labeled, colloidal gold labeled milk antigen, purification of colloidal gold milk antigen, colloidal gold protein particles Particle size identification and test strip coating. Specific steps are as follows:

[0030] 1. Preparation of antigen solution to be labeled

[0031]Preparation of milk antigen: take 100mL skimmed milk, filter it with an asbestos pla...

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Abstract

The invention provides a test strip for screening specific dairy allergen IgE. The test strip is coated with colloidal gold labeled dairy allergen. The colloidal gold labeling technique is utilized, the colloidal gold is used for labeling the specific dairy allergen (dairy antigen) firstly, then the labeled allergen is uniformly sprayed on a cellulose acetate membrane or a nitrocellulose membrane in a line manner, and the prepared test strip is used for allergen detection, the operation is simple and convenient, the result is accurate, the cost is low and other free detection can be realized.

Description

technical field [0001] The invention relates to the technical field of colloidal gold labeling, in particular to a test strip for screening specific milk allergen IgE and a preparation method thereof. Background technique [0002] Allergic disease is the most common disease in contemporary society. It is caused by widespread allergens. About 5-10% of the population in my country and 5-25% of the population in the United States and Europe have suffered from allergic infestation. Among them, young children And teenagers are particularly obvious, and harm is more serious. Allergic reaction (allergy) is a pathological immune response that causes physiological dysfunction and tissue damage when the immune body is exposed to the same allergen again. Involves various clinical departments, such as internal medicine including bronchial asthma, anaphylactic shock, serum sickness, connective tissue disease, glomerulonephritis, migraine, etc., surgery including transplant rejection, oto...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/558
CPCG01N33/531G01N33/558G01N33/68G01N2800/24
Inventor 蒋琳
Owner BIOSINO BIO TECH & SCI
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