A kind of rapid detection specific antibody IGE kit and preparation method thereof
A kit and a specific technology are applied in the field of rapid detection of specific antibody IgE kits and their preparation, which can solve problems such as low sensitivity, and achieve the effects of good stability, convenient portability and simple operation.
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Embodiment 1
[0072] The preparation of embodiment 1 allergen-specific antibody IgE rapid detection kit
[0073] 1. Preparation of catalase-labeled allergen
[0074] (I) All the allergen combination I, allergen combination II, allergen combination III and allergen combination IV were diluted to 5 μg / ml.
[0075] in:
[0076] Allergen combination I is an inhalation group of allergens, including: house dust mite / dust mite, artemisia pollen, ragweed pollen, dog hair / cat hair, cockroach, lawn pollen, mold mixture (Cladospora / Aspergillus fumigatus / Alternaria), tree pollen combination (willow / triangle poplar / juniper / English sycamore / poplar / elm) allergens;
[0077] Allergen combination II is an inhalation group of allergens, including: spring pollen I (cedar / fir / poplar / elm / willow), spring pollen II (birch / maple / oak / walnut / rape), Polyvalent fungi I (Penicillium chrysogenum / A. fungus / baker's yeast), polyvalent fungi III (Chemophagocystis sp. English sycamore / white wax / elm) allergens;
[0078...
Embodiment 2
[0108] Embodiment 2 quality inspection
[0109] The quality standard of the kit of the present invention is based on three parts of the "Pharmacopoeia" of the People's Republic of China, and the minimum detection limit, negative reference product compliance rate, positive reference product compliance rate, and precision all meet the national standard (Table 1).
[0110] Table 1: Allergen-specific antibody IgE rapid detection kit quality standards and tests
[0111]
[0112]
Embodiment 3
[0113] Embodiment 3 stability investigation
[0114] The allergen-specific antibody IgE detection kit was placed at 37°C for 8 days, and then tested with standard serum, CV<15% (Table 2).
[0115] Table 2: Stability Test
[0116]
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