31 results about "Allergen extract" patented technology

Pro-toothpaste compositions formulated to receive a volume of allergen solution to provide toothpastes that exhibit efficacy for oral mucosal immunotherapy (OMIT) and acceptable consumer and product stability properties, along with kits of pro-toothpaste, allergen/extracts of allergens, and optionally compounding means and/or specialized toothbrushes are provided. Toothpastes suitable and effective for OMIT and methods for managing allergic symptoms and for reducing risk of allergy in people without symptoms employing the pro-toothpastes, toothpastes and kits of the invention are also disclosed.

The invention relates to a water-containing liquid bacterin preparation and the purpose thereof for treating and preventing allergic reaction. The preparation contains an allergen extract which exists in water-containing liquid or suspension containing a protein stabilizer and derives from one or more kinds of artemisia plant pollen.

The invention relates to a London plane tree pollen allergen extract and an extracting solution and a preparation method thereof. The amino acid sequences of London plane tree pollen contained in theLondon plane tree pollen allergen extract are shown in SEQ ID NO.4 and SEQ ID NO.5, and the extracting solution can be prepared by London plane tree pollen collection, drying, degreasing, extracting,ultra-filtration concentrating, freeze-drying, re-dissolving and other process methods. The London plane tree pollen allergen extracting solution has the characteristic of high specificity, and the London plane tree pollen component is fully extracted, the total biological titer is stable, the validity period is long, and the aseptic effect is good. Its stock solution can be effectively used for skin prick test diagnosis of allergic reaction diseases, can be used for in-vitro basophil activation test diagnosis, intradermal test diagnosis and specific immunotherapy after proper dilution (10-2 to 10-20), and can effectively diagnose and carry out specific immunotherapy on allergic reaction diseases induced by London plane tree pollen.

The present invention provides a periplaneta americana allergen preparation production method, which comprises: (1) inactivating periplaneta americana in a refrigerator, removing intestinal tracts, washing with 75% ethanol, carrying out air-drying, and placing in the refrigerator so as to be spare; (2) taking an appropriate amount of the frozen periplaneta americana, and grinding into powder in liquid nitrogen; (3) degreasing with acetone, placing into the refrigerator to freeze, and carrying out freeze-drying in a vacuum freeze dryer to obtain powder; (4) weighing the periplaneta americana powder, adding an extraction solution, carrying out centrifugation, and sucking the supernatant, wherein the supernatant is a periplaneta americana allergen extract; (5) adopting specific antibodies to identify the content and the proportion of the main allergen in the periplaneta americana allergen crude extract; and (6) according to the identification result in the step (5), adding recombinant protein expressed by yeast or insects. With the periplaneta americana allergen crude extract extracted by using the method, the main allergen protein of the periplaneta americana can be obtained to the maximal degree, the extract has good repeatability, and the error is less than 30%.

The invention relates to processes for producing semi-purified and purified allergen extracts and pharmaceutical compositions and vaccines for use in the diagnosis and treatment of allergy. In one aspect of the invention, a process for producing a depigmented allergen extract is provided, the process comprising: a) basifying a native allergen extract; and b) removing molecules having a molecular size of less than 3.5 kDa; and c) adjusting the pH to neutrality; thereby to produce a depigmented allergen extract.

The invention provides a fraxinus pennsylvanica pollen allergen extract and immersion liquid of the fraxinus pennsylvanica pollen allergen extract and a preparation method thereof. The fraxinus pennsylvanica pollen allergen extract contains an amino acid sequence, which is as shown in SEQ ID NO.4, of fraxinus pennsylvanica pollen allergen protein. The immersion liquid is prepared through technology methods of fraxinus pennsylvanica pollen collecting, drying, degreasing, extracting, ultrafiltration concentrating, freeze-drying, redissolving and the like, and main components of the immersion liquid are a fraxinus pennsylvanica pollen allergen, glycerin, sodium chloride, phenol and the like. The fraxinus pennsylvanica pollen allergen immersion liquid has the character of high specificity, a fraxinus pennsylvanica allergic protein component is fully extracted, a total biological value is stable, a validity period is long, and an aseptic effect is good; and stoste of the immersion liquid ofthe fraxinus pennsylvanica pollen allergen extract can be effectively used for skin prick test diagnosis of allergic reaction diseases, and can be used for in-vitro basophilic granulocyte activationtest diagnosis and specific immunotherapy after being properly diluted so that the allergic reaction diseases induced by fraxinus pennsylvanica pollen can be effectively diagnosed, and can be subjected to specific immunotherapy.

The invention relates to novel immunogenic polypeptides identified in house dust mites and storage mites, which have the potential to be used in allergy immunotherapy, for diagnostic purposes, eventually via production of antibodies binding the polypeptide or for characterising allergen extracts of house dust mites and storage mites.

The invention belongs to the field of clinical laboratory diagnostics, and relates to an allergen-specific IgE antibody detection kit and a preparation method thereof. The reagent strip comprises a membrane strip, a sample pad, a colloidal gold pad, a water absorption pad and a PVC plate; the membrane strip is provided with at least one detection line, and the detection line is coated with an allergen extracting solution; the coating concentration of the allergen extracting solution is 0.5 to 5 mg/mL. The colloidal gold reagent strip and the kit disclosed by the invention can be used for detecting fingertip blood and whole blood, so that the bedside detection of allergens in a real sense is realized; the patient can be diagnosed in time, and even if the patient himself/herself can buy a reagent for self-inspection, so that the possibility is provided for early discovery and early treatment of more allergic disease patients.

The present invention relates to a purified veterinary allergen extract enriched. In particular, the invention relates to a purified veterinary allergen extract enriched with Der f 15 and Der f 18. The invention further relates to use of the allergen extract as a veterinary product, and its use in treating allergy, in particular house dust mite allergy in mammals, more particularly, dogs.

The invention discloses a high-efficiency wheat flour allergen extracting agent and a using method thereof, relating to the field of protein extraction, and aiming to provide a high-efficiency wheat allergen extracting agent, which has a high extraction rate and ensures high allergic immune activity of extracted wheat allergen, and the using method thereof. The high-efficiency wheat flour allergenextracting agent comprises a citrate phosphate buffer solution, in which the concentration of citric acid is 3 to 5 mmoL/L, and the concentration of phosphate is 70.3 to 147.3 mmoL/L, and the pH of the citrate phosphate buffer solution is 7.0 to 9.0. The using method of the high-efficiency wheat flour allergen extracting agent comprises the steps of weighing, extracting and detecting. The contentof allergen extracted by the high-efficiency wheat flour allergen extracting agent disclosed by the invention is 59.4%-106.3%, 54.5%-100% and 70%-120% higher than that of a phosphate buffer solution,tri(hydroxymethyl) amino methane hydrochloric acid and a Coca's solution, respectively; and the allergen obtained with the extracting agent has a rich variety of allergen subunits and high allergic immune activity.

The present invention relates to the virus elimination and deactivation process in preparing dermatophagoide allergen immersion, and viruses are eliminated and deactivated by means of several combined methods. Specifically, the combined process includes: 1.dry heating process including dry roasting of the instrument at 300 deg.c, heating culture medium and extracting buffer liquid at 80 deg.c for 72 hr, verifying the dry heating case for deactivating virus at least every half year and performing bacteria-free operation; 2. filtering process to filter allergen extracting liquid with filtering membrane to eliminate viruses of molecular weight greater than 100 KDa; 3. ultrasonic process to deactivate viruses via single cyclic path exposed to microwave energy; 4. chromatographic process to eliminate viruses of molecular weight greater than 100 KDa with Sephadex S-100 molecular sieve; and 5. verifying all the virus eliminating and deactivating processes.

The invention provides a process for the standardization of subcutaneous allergen immunotherapy formulas resulting in increasing availability and decreasing costs, allowing for expanded and improved relief for a greater number of allergy sufferers.

This invention provides a lower cost treatment to increase availability of the benefits of subcutaneous immunotherapy via standardization of most common seasonal or year round allergens for treatment. Standardizing a formula containing multiple allergen types allows a large population of potential users to benefit from a wide spectrum of included allergen sensitivities. Mass production of non-customized formulas allows for cost reduction, which breaks down a major barrier to current demand. The availability of non-individualized reduced allergen extract concentration strength formula allows for cost-effective availability in less-specialized delivery channels, which breaks down the other major barrier to current demand.

The present invention discloses processes for producing native, depigmented and polymerised allergen extracts. The invention further discloses extracts produced via the processes, and pharmaceutical and vaccine compositions comprising the extracts, for diagnosis and treatment of allergy.