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Test strip for screening specific insulin allergen ige and preparation method thereof

A technique for insulin and test strips, which is applied in the field of test strips for specific insulin allergen IgE screening and preparation thereof, can solve the problems of cumbersome operation, long test time, poor sensitivity and precision, etc. Inexpensive and accurate results

Active Publication Date: 2016-01-13
BIOSINO BIO TECH & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are disadvantages of cumbersome operation, poor sensitivity and precision, and long test time

Method used

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Examples

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Embodiment Construction

[0027] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available products.

[0028] Example The test strip used for the screening of specific insulin allergen IgE and its preparation method

[0029] The preparation of test strips for IgE screening of specific insulin allergens includes the preparation of the antigen solution to be labeled, the preparation of the colloidal gold solution to be labeled, the colloidal gold labeled insulin antigen, the purification of the colloidal gold insulin antigen, and the purification of the colloidal gold protein particles. Particle size identification and coating of test strips. Specific steps are as follows:

[0030] 1. Preparation of antigen solution to be labeled

[0031] ...

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Abstract

The invention provides a test strip for screening specific insulin allergens IgE (Immunoglobulin E). The test strip is coated with an insulin antigen marked by colloidal gold. The test strip utilizes a colloidal gold marking technology; firstly, the colloidal gold mark is used for marking the specific insulin allergens; then the marked allergens are uniformly sprayed on a cellulose acetate membrane or a nitrocellulose membrane to prepare a test strip product to carry out allergen detection; the test strip is simple and convenient to operate, accurate in result and low in cost and can realize multi-term free detection.

Description

technical field [0001] The invention relates to the technical field of colloidal gold labeling, in particular to a test strip for screening specific insulin allergen IgE and a preparation method thereof. Background technique [0002] Allergic disease is the most common disease in contemporary society. It is caused by widespread allergens. About 5-10% of the population in my country and 5-25% of the population in the United States and Europe have suffered from allergic infestation. Among them, young children And teenagers are particularly obvious, and harm is more serious. Allergic reaction (allergy) is a pathological immune response that causes physiological dysfunction and tissue damage when the immune body is exposed to the same allergen again. Involves various clinical departments, such as internal medicine including bronchial asthma, anaphylactic shock, serum sickness, connective tissue disease, glomerulonephritis, migraine, etc., surgery including transplant rejection, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543
CPCG01N33/531G01N33/543G01N33/558G01N2800/24
Inventor 蒋琳
Owner BIOSINO BIO TECH & SCI
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