Serum allergen specific IgE detection method based on micro-fluidic chip and protein chip

A protein chip and microfluidic chip technology, applied in the field of biomedicine, can solve the problems of inability to achieve blood volume, limited blood collection, immune response, etc., to achieve large-scale epidemiological analysis, reduce the amount of use and detection time. , the effect of reducing usage
CN105223367AActive Publication Date: 2016-01-06FUDAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
FUDAN UNIV
Publication Date
2016-01-06

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Abstract

The invention belongs to the field of biomedicine, and relates to a serum allergen specific IgE detection method based on micro-fluidic chip and protein chip. Micro fluidic chip only need a trace amount of sample and is capable of quickly carrying out analysis; protein chip can perform high-flux analysis, and the provided method adopts the micro fluidic chip and protein chip and is developed on an antigen-antibody specific immunity response principle. The micro fluidic chip and protein chip are combined together to detect the allergen specific IgE in a clinical serum sample of an allergy sufferer. The results show that the provided method can effectively reduce the using amount of serum for detection. So the method is suitable for infants or children, who have little peripheral blood. Moreover, the using amount of detection reagents and detection time are both reduced. Compared with the prior art, the provided method has the characteristics of rapidness, high flux, reduced amount of serum, high efficiency, portability, and low cost. The method is especially suitable for analysis of large-scale epidemic disease.
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Description

Technical field

[0001] The invention belongs to the field of biomedicine, and relates to a serum immunological detection method, in particular to a method for detecting serum allergen-specific IgE based on a microfluidic chip and a protein chip. Background technique

[0002] The prior art discloses allergies, that is, allergic reactions caused by the interpretation of foreign antigens as harmful objects (such as bacteria, pollen, dust mites, cockroaches, dust, etc.). Allergies activate macrophages in immune cells and release histamine and prostaglandin. Histamine and prostaglandin will cause a series of effects such as microvascular expansion, increased vascular permeability, itching, smooth muscle contraction and reflex; The clinical symptoms are mainly allergic rhinitis, allergic asthma, allergic gastroenteritis and eczema, urticaria, macula, papulosis, scratch syndrome, atopic dermatitis, wind rash, skin itching and other allergic skin disease. Allergic asthma is a relativel...

Claims

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