Serum allergen specific IgE detection method based on micro-fluidic chip and protein chip

Abstract

The invention belongs to the field of biomedicine, and relates to a serum allergen specific IgE detection method based on micro-fluidic chip and protein chip. Micro fluidic chip only need a trace amount of sample and is capable of quickly carrying out analysis; protein chip can perform high-flux analysis, and the provided method adopts the micro fluidic chip and protein chip and is developed on an antigen-antibody specific immunity response principle. The micro fluidic chip and protein chip are combined together to detect the allergen specific IgE in a clinical serum sample of an allergy sufferer. The results show that the provided method can effectively reduce the using amount of serum for detection. So the method is suitable for infants or children, who have little peripheral blood. Moreover, the using amount of detection reagents and detection time are both reduced. Compared with the prior art, the provided method has the characteristics of rapidness, high flux, reduced amount of serum, high efficiency, portability, and low cost. The method is especially suitable for analysis of large-scale epidemic disease.

Description

Technical field [0001] The invention belongs to the field of biomedicine, and relates to a serum immunological detection method, in particular to a method for detecting serum allergen-specific IgE based on a microfluidic chip and a protein chip. Background technique [0002] The prior art discloses allergies, that is, allergic reactions caused by the interpretation of foreign antigens as harmful objects (such as bacteria, pollen, dust mites, cockroaches, dust, etc.). Allergies activate macrophages in immune cells and release histamine and prostaglandin. Histamine and prostaglandin will cause a series of effects such as microvascular expansion, increased vascular permeability, itching, smooth muscle contraction and reflex; The clinical symptoms are mainly allergic rhinitis, allergic asthma, allergic gastroenteritis and eczema, urticaria, macula, papulosis, scratch syndrome, atopic dermatitis, wind rash, skin itching and other allergic skin disease. Allergic asthma is a relativel...

AI Technical Summary

Problems solved by technology

Skin pricking is currently a clinically used in vivo allergen detection method, but this method has disadvantages, such as, it may cause an immune response in the body, and the detection time is long, etc.; the UniCAP automatic in vitro diagnostic system developed by the Swedish Pharmacia Company is At present, the most advanced laboratory system for checking allergens in the world has been confirmed by the International Clinical Laboratory Standards Committee. This method has been confirmed by the World Health Organization for its high safety and accurate and reliable test results, and is known internationally as "The gold standard for allergen detection", however, the practice shows that the disadvantage of this method is that the detection requires a relatively large amount of serum, 40 μl of serum is required to detect a sample, especially for infants and young children, the most convenient way is usually to draw fingertip blood or earlobe For blood testing, due to the limited amount of blood taken, the blood volume required by the UniCAP method cannot be achieved.

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