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Test strip for specific egg white allergen ige screening and preparation method thereof

A test strip and allergen technology, which is applied to the test strip for specific egg white allergen IgE screening and the field of preparation thereof, can solve the problems of cumbersome operation, poor sensitivity and precision, long test time and the like, and achieves easy operation. , accurate results, low cost effect

Active Publication Date: 2016-03-23
BIOSINO BIO TECH & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are disadvantages of cumbersome operation, poor sensitivity and precision, and long test time

Method used

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Examples

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Embodiment Construction

[0027] The following examples are used to illustrate the present invention, but not to limit the scope of the present invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available products.

[0028] Example Test strips used for screening of specific egg white allergen IgE and preparation method thereof

[0029] The preparation of test strips for IgE screening of specific egg white allergens includes: preparation of antigen solution to be labeled, preparation of colloidal gold solution to be labeled, colloidal gold-labeled egg white antigen, purification of colloidal gold egg white antigen, and colloidal gold Identification of protein particle size and coating of test strips. Specific steps are as follows:

[0030] 1. Preparation of antigen solution to be labeled

[0031] Preparation of egg white antigen: Take an egg, pour the egg white into a Erl...

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Abstract

The invention provides a test strip for screening specific albumen allergens IgE (Immunoglobulin E). The test strip is coated with an albumen antigen marked by colloidal gold. The test strip utilizes a colloidal gold marking technology; firstly, the colloidal gold mark is used for marking the specific albumen allergens; then the marked allergens are uniformly sprayed on a cellulose acetate membrane or a nitrocellulose membrane to prepare a test strip product to carry out allergen detection; the test strip is simple and convenient to operate, accurate in result and low in cost and can realize multi-term free detection.

Description

Technical field [0001] The invention relates to the technical field of colloidal gold labeling, in particular to a test strip for screening specific egg white allergen IgE and a preparation method thereof. Background technique [0002] Allergic disease is the most common disease in contemporary society. It is caused by widespread allergens. About 5-10% of the population in my country, and about 5-25% in the United States and Europe have suffered allergic intrusion. And young people are particularly obvious, and the harm is more serious. Allergic reaction (allergy) is a pathological immune response in which the immune body is exposed to the same allergen again, and the physiological dysfunction and tissue damage are caused by an over-reaction. Involved in various clinical departments, such as internal medicine including bronchial asthma, anaphylactic shock, serum sickness, connective tissue disease, glomerulonephritis, migraine, etc., surgery including transplant rejection, otolar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/532
CPCG01N33/558G01N33/58G01N2800/24
Inventor 蒋琳
Owner BIOSINO BIO TECH & SCI
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