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Diagnostic kit for determination of serum total IgE, preparation method and application method

A diagnostic kit and the technology of the kit, applied in the field of immunoassay medicine, can solve the problems of environmental pollution of radioimmunoassay, poor repeatability of enzyme immunoassay, easy occurrence of "hook effect, etc., and achieve shortened detection time and strong anti-interference ability. , the effect of low cost

Inactive Publication Date: 2012-11-28
天津中企华科生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The problem to be solved by the present invention is to provide a serum total IgE homogeneous luminescent immunoassay diagnostic kit and its preparation and use method, so as to solve the cumbersome washing steps of the existing heterogeneous immunoassay kit; meanwhile, to overcome the environmental pollution of radioimmunoassay, The defect of short shelf life overcomes the poor repeatability of enzyme immunoassay and the defect of "hook effect" prone to occur

Method used

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  • Diagnostic kit for determination of serum total IgE, preparation method and application method
  • Diagnostic kit for determination of serum total IgE, preparation method and application method
  • Diagnostic kit for determination of serum total IgE, preparation method and application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] A homogeneous luminescent immunoassay diagnostic kit for serum total IgE, comprising:

[0069] (1) IgE standard (calibrator): 6 concentrations, 1000IU / ml, 500IU / ml, 200IU / ml, 100IU / ml, 50IU / ml and 0IU / ml;

[0070] (2) Biotin-labeled mouse anti-human IgE monoclonal antibody solution: the concentration is 0.447 μg / ml; the mouse anti-human IgE monoclonal antibody was purchased from Anti-IgE antibody (Mouse monoclonal[4C3]to IgE) from abcam Company, Cat. No. :ab106494;

[0071] (3) Acceptor microsphere solution coated with rabbit anti-human IgE polyclonal antibody: the concentration is 10 μg / ml; acceptor microspheres were purchased from Platinum Elmer Co., Ltd., catalog number 6762001; rabbit anti-human IgE polyclonal antibody was purchased from Anti-IgE antibody (Rabbit polyclonal to IgE) from abcam company, catalog number: ab75673.

[0072] (4) Streptavidin-labeled donor microsphere solution at a concentration of 50 μg / ml, purchased from Platinum Elmer Co., Ltd., Cat. N...

Embodiment 2

[0117] Such as figure 2 Shown, a kind of using method of the kit prepared in embodiment 1 comprises the steps:

[0118] (1) Take out the IgE standard, biotin-labeled mouse anti-human IgE monoclonal antibody solution, acceptor microsphere solution coated with rabbit anti-human IgE polyclonal antibody, and streptavidin-labeled donor microsphere solution , equilibrate to room temperature for 20 minutes; mark on the homogeneous luminescent 96-well reaction plate;

[0119] (2) Take the reaction plate, add 10 microliters of serum to be tested or IgE standard, 50 microliters of biotin-labeled mouse anti-human IgE monoclonal antibody solution and 50 microliters of rabbit anti-human IgE polyclonal into the microwells successively. Antibody receptor microsphere solution, mix well and incubate at 37°C for 15 minutes to fully combine antigen and antibody;

[0120] (3) Add 100 microliters of streptavidin-labeled donor microspheres to the microwells in the dark, mix well and incubate at ...

Embodiment 3

[0128] Performance testing of the kit prepared by the present invention.

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Abstract

The invention provides a diagnostic kit for determination of serum total IgE, a preparation method and an application method. The kit comprises an IgE standard substance, a biotin labeled rate anti-human IgE monoclonal antibody solution, a rabbit anti-human IgE polyclonal antibody coated acceptor microspheres solution and a streptavidin donor microspheres solution. The kit provided by the present invention solves a tedious washing step of current heterogeneous immunoassay kits, overcomes the defects of environmental pollution caused by radioimmunoassay and short shelf life, and overcomes the defects of poor repeatability of enzyme immunoassay analysis and easy hook effect generation, and the detection precision is higher than that of immuno-turbidimetric analysis.

Description

technical field [0001] The invention belongs to the field of immunoanalysis medicine, and in particular relates to a serum total immunoglobulin E (total IgE) assay diagnostic kit and a preparation and use method. Background technique [0002] Allergic disease refers to a pathological immune response that occurs when the body is stimulated by the same antigen again after the body responds to certain antigens for the first time, mainly due to physiological dysfunction or tissue damage. Clinical allergic diseases mainly refer to type I allergic reactions, the mechanism of which is that allergens (allergens) can stimulate B lymphocytes to produce specific IgE (sIgE) antibodies after entering the body. Sensitized state. When the same allergen enters the sensitized body again, it can combine with specific IgE antibodies to trigger cell activation and produce an allergic reaction. In recent years, with the development of social economy, the improvement of living environment and t...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N21/64
Inventor 李会强苑凤君周宇捷张燕玲
Owner 天津中企华科生物科技发展有限公司
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