Diagnostic kit for determination of serum total IgE, preparation method and application method
A diagnostic kit and the technology of the kit, applied in the field of immunoassay medicine, can solve the problems of environmental pollution of radioimmunoassay, poor repeatability of enzyme immunoassay, easy occurrence of "hook effect, etc., and achieve shortened detection time and strong anti-interference ability. , the effect of low cost
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Embodiment 1
[0068] A homogeneous luminescent immunoassay diagnostic kit for serum total IgE, comprising:
[0069] (1) IgE standard (calibrator): 6 concentrations, 1000IU / ml, 500IU / ml, 200IU / ml, 100IU / ml, 50IU / ml and 0IU / ml;
[0070] (2) Biotin-labeled mouse anti-human IgE monoclonal antibody solution: the concentration is 0.447 μg / ml; the mouse anti-human IgE monoclonal antibody was purchased from Anti-IgE antibody (Mouse monoclonal[4C3]to IgE) from abcam Company, Cat. No. :ab106494;
[0071] (3) Acceptor microsphere solution coated with rabbit anti-human IgE polyclonal antibody: the concentration is 10 μg / ml; acceptor microspheres were purchased from Platinum Elmer Co., Ltd., catalog number 6762001; rabbit anti-human IgE polyclonal antibody was purchased from Anti-IgE antibody (Rabbit polyclonal to IgE) from abcam company, catalog number: ab75673.
[0072] (4) Streptavidin-labeled donor microsphere solution at a concentration of 50 μg / ml, purchased from Platinum Elmer Co., Ltd., Cat. N...
Embodiment 2
[0117] Such as figure 2 Shown, a kind of using method of the kit prepared in embodiment 1 comprises the steps:
[0118] (1) Take out the IgE standard, biotin-labeled mouse anti-human IgE monoclonal antibody solution, acceptor microsphere solution coated with rabbit anti-human IgE polyclonal antibody, and streptavidin-labeled donor microsphere solution , equilibrate to room temperature for 20 minutes; mark on the homogeneous luminescent 96-well reaction plate;
[0119] (2) Take the reaction plate, add 10 microliters of serum to be tested or IgE standard, 50 microliters of biotin-labeled mouse anti-human IgE monoclonal antibody solution and 50 microliters of rabbit anti-human IgE polyclonal into the microwells successively. Antibody receptor microsphere solution, mix well and incubate at 37°C for 15 minutes to fully combine antigen and antibody;
[0120] (3) Add 100 microliters of streptavidin-labeled donor microspheres to the microwells in the dark, mix well and incubate at ...
Embodiment 3
[0128] Performance testing of the kit prepared by the present invention.
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