Method for measuring allergic response

A kind of allergy, allergy technology, applied in the field of food allergy, allergy, can solve the problem of no food allergy progress and so on

Inactive Publication Date: 2008-03-12
NV NUTRICIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No specific progression for food allergies

Method used

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  • Method for measuring allergic response
  • Method for measuring allergic response

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Induction of degranulation with serum from allergic and non-allergic patients

[0048] Threshing Models for Product Development and Alternative Allergic Types

[0049] The following systems are available for the detection of food allergens and for the determination of reactivity tests in allergic patients:

[0050] Degranulated cells are cultured in Geneticin-free medium (selectable marker) for 1 to 2 days and cells are sensitized overnight with preferably diluted IgE from one source (purified individual sera and / or pooled sera). In case of product development, the serum is preferentially isolated from allergy sufferers, especially milk or egg allergy donors. The corresponding sensitized cells containing IgE were washed several times with PIPES buffer (and / or Tyrode buffer) to remove unbound IgE. The cells are then stimulated for 30 minutes to 1 hour with an allergen, which may be a purified allergen, but commercial food of the food composition to be deve...

Embodiment 2

[0060] Example 2: Sera from a Patient with a Cow's Milk Allergy (CMA), Not a Cow's Allergy Degranulation induced by sera from allergic patients and sera from non-allergic patients.

[0061] The method is carried out as described in embodiment 1. The results of the test are shown in Table 2, which show that the method is suitable for detecting allergic reactions to products with complete nutrition in individual patients. Allergic patients who were not allergic to cow's milk did show negative results, demonstrating the reliability of this method.

[0062] Table 2

[0063]

Embodiment 3

[0064] Embodiment 3: complete kit:

[0065] The complete kit includes:

[0066] (1) One contains 1 gram of Nutrilon 2 TM (Nutricia, Zoetermeer, Netherlands) packaging;

[0067] (2) One contains 1 gram of Nutrilon Hypo-Allergeen TM (Nutricia, Zoetermeer, Netherlands) packaging;

[0068] (3) One contains 1 gram of Neocate TM (Nutricia, Zoetermeer, Netherlands) packaging;

[0069] (4) 4-Methylumbelliferyl-N-acetyl-β-D-glucosaminide (from Fluka, Sigma-Aldrich).

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Abstract

The present invention discloses a method for measuring allergic reaction with measuring the degranulation degree when the human IgE sensitized cell is activated by the allergen in the food.

Description

technical field [0001] The present invention relates to the field of allergy, especially food allergy. The present invention provides an in vitro method for determining whether a subject exhibits an allergic reaction when exposed to a particular food product. The invention also provides a method for developing food products with good allergenicity. Background technique [0002] Food allergy is a harmful immune reaction in susceptible individuals caused by ingestion of allergy-inducing substances in food and can cause dermatitis, asthma, digestive tract obstruction, anaphylactic shock, etc. Wait. Food allergy was originally called type I allergy, an immune disorder mediated by IgE antibodies that react with ingested food allergens. The number of patients suffering from food allergy has continued to rise in recent years, and the phenomenon of food allergy has caused serious problems in the fields of medicine and the food industry. [0003] Foods that trigger an allergic re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50C12Q1/34
Inventor V·S·C·特里戈特J·加森
Owner NV NUTRICIA
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