Anti-drought ultraviolet resistant long-storage cicadae penicillium notatum strain and application thereof

A Paecilomyces cicadae, anti-ultraviolet technology, applied in applications, fungi, microorganism-based methods, etc., can solve the problems of ecological environment damage, pest resistance enhancement, etc., to achieve no damage to ecological balance, good effect, and production cost. low effect

Inactive Publication Date: 2007-09-26
GUIZHOU UNIV
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

This poses a serious challenge to conventional wisdom and bad practices in pest control
Long-term use of chemical pesticides will lead to side effects such as damage to the ecological environment and increased pest resistance

Method used

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Examples

Experimental program
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Embodiment Construction

[0016] Pick a small amount of spore powder from the cicadae specimens collected from Maolan Nature Reserve in Guizhou Province with a sterile inoculation needle, put them on the Sabouraud medium with penicillin and streptomycin, and place them in a constant temperature incubator at 25°C , cultured for 3-5 days, picked a small amount of mycelium of a single colony and transferred it to a test tube, and obtained the stress resistance of the entomogenic fungus Paecilomyces cicadae by using ultraviolet radiation for mutagenesis and screening with polyethylene glycol-6000. sex mutants. The spores of the strain can grow in the medium containing polyethylene glycol-6000, the maximum concentration of polyethylene glycol that can be tolerated is 45%, and the osmotic pressure of the medium is lower than -2.0Mpa. Its spore death rate is less than 20% after irradiation time 5 minutes with 30 watts ultraviolet lamp 40 centimeters.

[0017] Continuously transfer 10 generations on the PDA m...

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Abstract

The invention discloses a strain resisting drought, resisting ultraviolet and storage cicada pecilocin (Paecilomyces cicadidae Miquel samson GZDXIFR-M3L) and appliance, which is characterized by the following: growing in culture medium of carbowax -6000; setting the highest tolerance density at 45%; using 30 watt ultraviolet lamp to irradiate at 40 cm site; irradiating at 5 min; making the survival rate bigger than 80%; possessing stronger resistance for imperfect environment. This invention can prevent and cure aphid and powder lice with stab and suck type mouth parts.

Description

technical field [0001] The invention relates to an entomogenic fungus Paecilomyces cicadae and its application. Background technique [0002] Cicada is a traditional and precious traditional Chinese medicinal material in my country. Su Song of the Song Dynasty described the cicada in the "Bencao Tujing": "There is a kind of cicada in Sichuan today. It has a corner on its shell head, which is like a corolla. It is called a cicada. Flowers... are the most amazing medicines". It is also recorded in the herbal medicines of the past dynasties that the host insects of cicadae mainly include cicadae, mole crickets and black grasshoppers. In recent years, researchers have isolated and purified a fungus named Paecilomyces cicadidae Miquel samson from cicada flowers. The conidia stage of cicadae was also called Isaria cicadae Miquel, the conidia stage was called anamorph, and the astheca stage was called telemorph. In nature, the sexual type is very rare, and the asexual type is oft...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04C12R1/79
Inventor 刘爱英邹晓赵杰宏李忠
Owner GUIZHOU UNIV
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