Anti-drought ultraviolet resistant long-storage cicadae penicillium notatum strain and application thereof
A Paecilomyces cicadae, anti-ultraviolet technology, applied in applications, fungi, microorganism-based methods, etc., can solve the problems of ecological environment damage, pest resistance enhancement, etc., to achieve no damage to ecological balance, good effect, and production cost. low effect
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[0016] Pick a small amount of spore powder from the cicadae specimens collected from Maolan Nature Reserve in Guizhou Province with a sterile inoculation needle, put them on the Sabouraud medium with penicillin and streptomycin, and place them in a constant temperature incubator at 25°C , cultured for 3-5 days, picked a small amount of mycelium of a single colony and transferred it to a test tube, and obtained the stress resistance of the entomogenic fungus Paecilomyces cicadae by using ultraviolet radiation for mutagenesis and screening with polyethylene glycol-6000. sex mutants. The spores of the strain can grow in the medium containing polyethylene glycol-6000, the maximum concentration of polyethylene glycol that can be tolerated is 45%, and the osmotic pressure of the medium is lower than -2.0Mpa. Its spore death rate is less than 20% after irradiation time 5 minutes with 30 watts ultraviolet lamp 40 centimeters.
[0017] Continuously transfer 10 generations on the PDA m...
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