Method for producing feeding glucose oxidase by using rice processing by-products as raw materials

The technology of a glucose oxidase and production method is applied in the field of solid fermentation to produce feed glucose oxidase, which can solve the problems of high technical quality requirements for fermentation workers, high cost of fermentation equipment, high energy consumption in the fermentation process, etc., and achieve wide source of raw materials , Reduce the cost of raw materials, the effect of simple process

Inactive Publication Date: 2011-10-19
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, the production of glucose oxidase for feed uses the liquid submerged fermentation method, which has many shortcomings in specific applications, for example, the cost of fermentation equipment is high, and liquid submerged fermentation requires professional fermentation equipment, such as fermentation tanks, etc., a 30-ton The cost of the fermentation tank is up to 1 million; the fermentation process consumes a lot of energy and requires constant stirring; the technical quality of the fermentation workers is high; the three wastes (waste water, waste residue, and waste gas) cause serious environmental pollution (Journal of Microbiology 2009, 1 (29 ) p86-89; Food and Fermentation Industry 2003, 29 (6) p 87-91)
There is another significant disadvantage of the liquid fermentation method of glucose oxidase for feed production: the raw material used in the fermentation process is mainly high-quality soft white sugar, and the raw material cost is high ("Feed and Animal Husbandry" 2008 (7) p37-39)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Inoculate a 10 cm microbial culture dish with potato agar medium (PDA medium) with Penicillium pointis, which produces high glucose oxidase, and incubate at 28 °C for 40 h;

[0016] The preparation of the potato agar medium: boil the juice of 200 g of peeled potatoes for 30 min, filter through 4 layers of gauze, add 20 g of glucose and 15 g of agar to the filtrate, add distilled water to 1000 ml, sterilize at 115°C for 20 min, and spread the plate for standby;

[0017] ⑵ Seed culture: liquid seed medium formula: 5wt% high-quality soft white sugar, 0.1wt% KH2PO4, 0.1wt% NaCl, 0.02wt% MgSO4·7H2O, 0.2wt% NaNO3: 0.0015wt% FeCl3, 0.1wt% CaCl2, distilled water preparation , pH natural), after sterilizing at 115 ℃ for 20 min, the activated Penicillium pointis in the petri dish was transferred to the liquid seed medium, and the inoculation amount was 500 ml of liquid seed medium in a 10 cm petri dish; Cultivate until a large number of spores are formed;

[0018] (3) Prepar...

Embodiment 2

[0026] ⑴ Inoculate a 10 cm microbial culture dish with potato agar medium (PDA medium) with Penicillium pointis, which produces high glucose oxidase, and culture it at 30 °C for 36 h;

[0027] The preparation of the potato agar medium: boil the juice of 200 g of peeled potatoes for 30 min, filter through 4 layers of gauze, add 20 g of glucose and 15 g of agar to the filtrate, add distilled water to 1000 ml, sterilize at 115°C for 20 min, and spread the plate for standby;

[0028] ⑵ Seed culture: liquid seed medium formula: 10 wt% high-quality soft white sugar, 0.15wt% KH2PO4, 0.15wt% NaCl, 0.03 wt% MgSO4 7H2O, 0.3wt% NaNO3: 0.003wt% FeCl3, 0.15wt% CaCl 2 , the rest is water, the pH is natural, after sterilizing at 115 ℃ for 30 minutes, the activated Penicillium pointis in the petri dish is transferred to the liquid seed medium, and the inoculum amount is 1000 ml liquid seed medium for a 10 cm petri dish; inoculation Then culture at 30°C until a large number of spores are forme...

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PUM

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Abstract

The invention discloses a method for producing feeding glucose oxidase by using rice processing by-products as raw materials, which comprises the following steps of: (1) inoculation of penicillium notatum of glucose oxidase with high yield into a microorganism culture dish of 10cm filled with a PDA (Potato Dextrose Agar) culture medium for carrying out cultivation; (2) seed cultivation: sterilizing a liquid seed culture medium at a temperature of 115 to 121 DEG C for 20 to 30 min, and then transferring the activated penicillium notatum in the culture dish in the step (1) to the liquid seed culture medium; (3) preparation of a solid fermentation medium: mixing broken rice and rice bran according to the weight ratio of 10: (1-3), and cooking for 25 to 40 min at a temperature of 90 to 100 DEG C; (4) inoculating cultivation: after spreading the solid fermentation medium for cooling, inoculating penicillium notatum seeds for carrying out cultivation; and (5) after ending the solid fermentation, drying the culture medium at a temperature of 100 to 120 DEG C for 2-4h, and crushing to 40 to 80 meshes. The method disclosed by the invention has the advantages that sources of raw materials are wide, no three wastes (waste gas, waste water and industrial residual) are generated, the process is simple and the vigor of the glucose oxidase reaches up to 200 U.

Description

technical field [0001] The invention relates to a production method of glucose oxidase for feed, in particular to a method for producing glucose oxidase for feed by solid fermentation by using broken rice and rice bran as by-products of rice processing. Background technique [0002] At present, the production of glucose oxidase for feed uses the liquid submerged fermentation method, which has many shortcomings in specific applications, for example, the cost of fermentation equipment is high, and liquid submerged fermentation requires professional fermentation equipment, such as fermentation tanks, etc., a 30-ton The cost of the fermentation tank is up to 1 million; the fermentation process consumes a lot of energy and requires constant stirring; the technical quality of the fermentation workers is high; the three wastes (waste water, waste residue, and waste gas) cause serious environmental pollution (Journal of Microbiology 2009, 1 (29 ) p86-89; Food and Fermentation Indust...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/04C12R1/80
Inventor 杨涛林亲录刘俊文周慧
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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