Method for producing feeding glucose oxidase by using rice processing by-products as raw materials
The technology of a glucose oxidase and production method is applied in the field of solid fermentation to produce feed glucose oxidase, which can solve the problems of high technical quality requirements for fermentation workers, high cost of fermentation equipment, high energy consumption in the fermentation process, etc., and achieve wide source of raw materials , Reduce the cost of raw materials, the effect of simple process
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Embodiment 1
[0015] (1) Inoculate a 10 cm microbial culture dish with potato agar medium (PDA medium) with Penicillium pointis, which produces high glucose oxidase, and incubate at 28 °C for 40 h;
[0016] The preparation of the potato agar medium: boil the juice of 200 g of peeled potatoes for 30 min, filter through 4 layers of gauze, add 20 g of glucose and 15 g of agar to the filtrate, add distilled water to 1000 ml, sterilize at 115°C for 20 min, and spread the plate for standby;
[0017] ⑵ Seed culture: liquid seed medium formula: 5wt% high-quality soft white sugar, 0.1wt% KH2PO4, 0.1wt% NaCl, 0.02wt% MgSO4·7H2O, 0.2wt% NaNO3: 0.0015wt% FeCl3, 0.1wt% CaCl2, distilled water preparation , pH natural), after sterilizing at 115 ℃ for 20 min, the activated Penicillium pointis in the petri dish was transferred to the liquid seed medium, and the inoculation amount was 500 ml of liquid seed medium in a 10 cm petri dish; Cultivate until a large number of spores are formed;
[0018] (3) Prepar...
Embodiment 2
[0026] ⑴ Inoculate a 10 cm microbial culture dish with potato agar medium (PDA medium) with Penicillium pointis, which produces high glucose oxidase, and culture it at 30 °C for 36 h;
[0027] The preparation of the potato agar medium: boil the juice of 200 g of peeled potatoes for 30 min, filter through 4 layers of gauze, add 20 g of glucose and 15 g of agar to the filtrate, add distilled water to 1000 ml, sterilize at 115°C for 20 min, and spread the plate for standby;
[0028] ⑵ Seed culture: liquid seed medium formula: 10 wt% high-quality soft white sugar, 0.15wt% KH2PO4, 0.15wt% NaCl, 0.03 wt% MgSO4 7H2O, 0.3wt% NaNO3: 0.003wt% FeCl3, 0.15wt% CaCl 2 , the rest is water, the pH is natural, after sterilizing at 115 ℃ for 30 minutes, the activated Penicillium pointis in the petri dish is transferred to the liquid seed medium, and the inoculum amount is 1000 ml liquid seed medium for a 10 cm petri dish; inoculation Then culture at 30°C until a large number of spores are forme...
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