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Penicillium notatum strain and application thereof in biotransformation mulberry leaf flavonoid glycoside

A technology of mulberry leaf flavonoids and Penicillium pointis, applied in the field of microorganisms, can solve the problems of lack of basic data, unreported research, unstable yield and conversion rate, etc.

Inactive Publication Date: 2011-01-19
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the preparation of highly active aglycone by microbial or enzymatic conversion of mulberry leaf flavonoid glycosides
[0005] Although the existing technology shows that microorganisms can produce glycoside hydrolase, the yield and conversion rate reports are very unstable, and there is a lack of basic data on the application of mulberry leaf flavonoid glycoside conversion and directional hydrolysis to produce mulberry leaf flavonoid aglycone

Method used

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  • Penicillium notatum strain and application thereof in biotransformation mulberry leaf flavonoid glycoside
  • Penicillium notatum strain and application thereof in biotransformation mulberry leaf flavonoid glycoside
  • Penicillium notatum strain and application thereof in biotransformation mulberry leaf flavonoid glycoside

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] This example illustrates the screening procedure for Penicillium notatum LJ-2.

[0016] Collect multiple soil samples, put them into a conical flask with 50mL of sterile water, add about 20 glass beads, shake vigorously for 20 minutes to completely mash the soil, and let it stand still. Add 1mL of soil sample suspension into each 50mL sterile water Erlenmeyer flask, put it into a constant temperature shaking incubator and cultivate it at 37°C for 2 days. Take the suspension and spread it on the primary screening plate medium to carry out the primary screening of the enzyme-producing strains. The quality composition of the primary screening medium is: dipotassium hydrogen phosphate 1.0%, potassium dihydrogen phosphate 0.5%, ammonium sulfate 0.1%, sodium nitrate 0.05%, magnesium sulfate 0.05%, ferrous sulfate 0.005%, zinc sulfate 0.005%, agar 5%, pH 6.5, and added 1% rutin. The temperature is 35°C, and the cultivation time is 1 to 6 days. Select 20 strains that produce...

Embodiment 2

[0022]This example illustrates the method of using Penicillium notatum LJ-2 to carry out fermentation and enzyme production to produce glycoside hydrolase and its biotransformation of mulberry flavone glycosides to prepare highly active aglycones.

[0023] The mass ratio composition of the medium: 0.1% potassium hydrogen phosphate, 0.1% potassium dihydrogen phosphate, 0.1% ammonium sulfate, 0.01% sodium nitrate, 0.01% magnesium sulfate, 0.001% ferrous sulfate, 0.001% zinc sulfate, pH 5.0, and Add 1% rutin.

[0024] The spore suspension (mass concentration) of Penicillium notatum LJ-2 was inoculated in the culture medium with an inoculum amount of 1%, and cultured aerobically at a temperature of 25° C. for 1 day, that is, the enzyme production ended.

[0025] The flavonoid glucoside of mulberry leaves is dissolved to prepare a solution with a mass concentration of 1%, and the pH is adjusted to 4.0 with NaOH or HCl solution, which is used as a substrate for the enzyme-catalyzed ...

Embodiment 3

[0027] The method of this embodiment is the same as that of Embodiment 2, and the parameters of the method used are changed.

[0028] The mass ratio composition of the medium: dipotassium hydrogen phosphate 1.0%, potassium dihydrogen phosphate 0.5%, ammonium sulfate 0.1%, sodium nitrate 0.05%, magnesium sulfate 0.05%, ferrous sulfate 0.005%, zinc sulfate 0.005%, pH 6.0.

[0029] The spore suspension (mass concentration) of Penicillium notatum LJ-2 was inoculated in the culture medium at an inoculum size of 2%, and cultured aerobically at a temperature of 35° C. for 4 days, that is, the enzyme production ended.

[0030] The solution is prepared into a solution with a mass concentration of 3%, which is used as the substrate of the enzyme-catalyzed reaction. Add the substrate solution to the crude enzyme solution or wet bacteria obtained by filtering the fermentation broth, and adjust the pH to 6.5 with NaOH or HCl solution, at 35°C, with a rotation speed of 150r / min, and react f...

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Abstract

The invention discloses a penicillium notatum LJ-2 strain. The preservation date is July 14th, 2008, and the preservation registration number is CGMCC No.2590. The invention also discloses applicationof the penicillium notatum LJ-2 in preparing flavonoid glycosides by biotransformation mulberry leaf flavonoid glycoside.

Description

1. Technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a Penicillium point strain and its application in biotransformation of mulberry flavonoid glycosides. 2. Background technology [0002] Prior art: The medicinal use of mulberry resources has a long history in my country, and it is widely used as natural medicine. my country is the origin center of mulberry trees, and the germplasm types of mulberry trees are rich and diverse. There are 15 species and 4 varieties of Morus in my country. The National Germplasm Zhenjiang Mulberry Nursery now preserves more than 1,900 copies of various mulberry germplasms of 12 mulberry species and 3 varieties. Mulberry trees are distributed and cultivated in most areas of my country. The area of ​​mulberry gardens in the country is about 10 million mu, of which Jiangsu, Zhejiang, Sichuan and other major producing areas have the largest output. Mulberry leaves are the part with the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P17/06C12R1/80
Inventor 吴福安王俊陈明胜耿涛仇荣赵璐璐
Owner JIANGSU UNIV OF SCI & TECH
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