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Method for preparing antibody for detecting clenbuterol

A technology of clenbuterol and antibodies, which can be used in measuring devices, instruments, scientific instruments, etc., and can solve the problem of low ELISA titer

Inactive Publication Date: 2007-02-28
SHANGHAI HIGH SCHOOL
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

The antibodies used in these detection reagent strips all use heterologous protein (BSA) as the carrier, and the ELISA titer of the antibody is not high

Method used

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  • Method for preparing antibody for detecting clenbuterol
  • Method for preparing antibody for detecting clenbuterol
  • Method for preparing antibody for detecting clenbuterol

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Embodiment Construction

[0017] The following combination selects self-proteins to prepare "leptin" carrier antigens, and produces high-titer specific antibodies after immunization of animals. The purified antibodies are combined with colloidal gold to form immune gold, which is prepared into simple test strips. The present invention is described in detail through processes such as detection of test strip sensitivity, shelf life, comparison of operating methods, clinical testing, and parallel detection with monoclonal antibody gold standard test strips.

[0018] The technical route of the above process is as follows:

[0019] A. Antibody preparation: "Clenbuterol" + self-protein (albumin, whole serum and hemoglobin) → carrier "Clenbuterol" (antigen) → preparation of adjuvant antigen → immunization of rabbits → blood sampling to measure antibody titer (indirect ELISA method )→serum separation→antibody purification→antibody quantification.

[0020] B. Preparation of immunogold test strips: preparation ...

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Abstract

The invention relates to a method for preparing antibody used to check lean meat, wherein it comprises: collecting the animal blood and separating serum to prepare full-serum protein solution; the mixing the solution into carbonate baffle liquid; then adding azo liquid, to prepare carrier couple material; adding the carrier couple material and Freund's complete adjuvant into penicillium notatum bottle, vibrating it on mixer to be white color, to form CL Freund's complete adjuvant emulsion liquid; using emulsifying another carrier couple material and Freund's incomplete adjuvant; and planting the prepared CL Freund's complete adjuvant emulsion liquid on animal to be used in immunization; then separating serum to obtain antibody. The invention uses itself protein, especially the full-serum as the immunization carrier of CL, to make the immunity animal generate special antibody whose ELISA effect can reach more than 40thousand; and the immunity gold testing method built from said antibody can quickly test CL of urine sample, while its sensitivity is 40ng / ml.

Description

technical field [0001] The invention relates to a method for preparing an antibody, in particular to a method for preparing an antibody for detecting clenbuterol. Background technique [0002] Modern animal husbandry provides humans with an extremely rich variety of meat, poultry, milk, eggs and other foods with high nutritional value and high quality, which has made great contributions to human material life, improving people's health and prolonging human life. At the same time, modern scientific research has proved that due to the modernization of animal husbandry, a large number of antibiotics, hormones and other synthetic drugs are used, resulting in drug residues and hormone residues in other livestock and poultry foods. Through the food chain, drug residues and hormone residues pose danger and damage to human health and life, such as the increase in the incidence of cancer patients, the increase in the proportion of human birth deformities, the increase in human drug r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/535
Inventor 刘见
Owner SHANGHAI HIGH SCHOOL
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