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Application of Bacillus aryabhattai in preparation of acid soil conditioner

A technology of Bacillus aeruginosa, acid soil, applied in the field of microorganisms

Active Publication Date: 2019-07-30
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the core of the research is still the screening of strains with excellent performance

Method used

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  • Application of Bacillus aryabhattai in preparation of acid soil conditioner
  • Application of Bacillus aryabhattai in preparation of acid soil conditioner
  • Application of Bacillus aryabhattai in preparation of acid soil conditioner

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The acid-base tolerance of Bacillus Ascheri with the deposit number CCTCC AB 207736:

[0043] Alkali resistance test:

[0044] Inoculate the Bacillus abbeyii seed solution into LB medium with different initial pH according to the inoculation amount of 5%, culture at 30℃ and 200r / min, and take samples at regular intervals to measure OD 600 , The measurement is not ended until the late stabilization period. The initial pH gradient of the medium is set to 7, 8, 9, 10.

[0045] LB medium: 10g peptone, 5g yeast extract, 10g NaCl, 1000mL distilled water, pH7.0, autoclaved at 121°C for 20min.

[0046] Test results such as figure 1 with figure 2 The pH in the two groups of pH9 and pH10 has been in a downward trend from the beginning to 23h. It may be that Bacillus Ascheri produces some acidic substance to neutralize the alkali in the culture medium in order to make the environment suitable for spontaneous growth.

[0047] In the pH7 and pH8 groups, the pH kept rising, indicating that t...

Embodiment 2

[0054] Phosphorus-dissolving ability of Bacillus Ascheri with the deposit number CCTCC AB 207736:

[0055] The seed solution was inoculated into a liquid phosphorus-dissolving medium according to the inoculum amount of 5%, cultivated at 30℃, 200r / min, and then the available phosphorus (soluble phosphorus) content in the culture solution was determined by the molybdenum blue colorimetric method to determine the solubility The strength of phosphorus.

[0056] Phosphate dissolution medium (PKO medium): glucose 10.0g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.3g, KCl 0.3g, MgSO 4 ·7H 2 O 0.3g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 ·7H 2 O 0.3g, poorly soluble phosphorus source, 1000mL of water, pH 7.0, high pressure steam sterilization at 115°C for 30 minutes; poorly soluble phosphorus source and dosage: tricalcium phosphate 0.5%, phosphate rock powder 0.5%, egg yolk lecithin 0.2% (phosphoric acid 0.5% of tricalcium means that 0.5 g of tricalcium phosphate is added to 100 mL of medium, and the rest are the ...

Embodiment 3

[0063] The inhibitory effect of Bacillus asheri with the deposit number CCTCC AB 207736 on plant pathogens:

[0064] The inhibition zone method was used to determine the inhibitory ability of Bacillus asheri on pathogens. Dilute the activated Bacillus argentii seed solution appropriately and spread it on a PDA plate, and spread sterile water as a control test group. Punch holes on the PDA plate covered with pathogenic fungi, pick the bacterial clumps and inoculate them upside down on the PDA plate coated with Bacillus Ascheri, contact the plate with fungi, and place the plate at 30°C for 7 days. Subsequently, the diameter of the fungal colony was measured and the inhibition rate was calculated to analyze the antibacterial effect.

[0065] The plant pathogens selected in this experiment include banana fusarium wilt pathogen, potato scab pathogen, verticillium fusarium, Fusarium oxysporum (2 strains), Fusarium solani (2 strains), Fusarium, Cladosporium, Ash Botrytis, Myrothecium sp...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to application of Bacillus aryabhattai in preparation of an acidic soil conditioner. The Bacillus aryabhattai with the preservation number of CCTCC AB 207736 is used. The dissolving effect of the bacterial strain on an organic insoluble phosphorus source reaches 20% or above; the bacterial strain has a betterinhibition effect on pathogenic bacterium of fusarium; the bacteriostasis rate reaches 80% or above; the inhibition rate of the bacterial strain on pathogenic bacterium of banana wilt and potato scabcan reach 60% or above; and the inhibition rate of the bacterial strain on botrytis cinerea and lacca can reach about 60%. The bacterial strain has an obvious effect on improving the acid soil, and the pH of the soil before and after treatment is increased from 4.8 to 6.0. The Bacillus aryabhattai has obvious advantages in the aspects of biological control, soil fertilizer efficiency improvement,environmental bioremediation and the like, and has potential application value.

Description

Technical field [0001] The invention belongs to the field of microbial technology, and specifically relates to the application of Bacillus asheri in the preparation of acidic soil amendments. Background technique [0002] According to research reports, due to the increase in the use of chemical fertilizers in our country, the increasing dependence on chemical fertilizers has resulted in insufficient use of organic fertilizers and imbalanced soil nutrient ratios; soil compaction and soil quality decline; river and groundwater pollution, etc. problem. Microbial fertilizer has the characteristics of high fertilizer efficiency, non-toxic, non-pollution to the environment, low cost, and energy saving. It is the most effective substitute for chemical fertilizers. The rational development and utilization of microbial fertilizers is an important way for the sustainable development of agriculture in my country. In the past 30 years, the output and types of pesticides in my country have ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/14A01N63/00A01P3/00A01P21/00C12N1/20C12R1/07C09K109/00
CPCA01N63/00C09K17/14C09K2109/00C12N1/20
Inventor 吴鹏宇陈振民艾洪超任浩然汪兆璞龚泽奇王巧梅梁运祥
Owner HUAZHONG AGRI UNIV
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