Sea yeast for diseases biological control of postharvest fruits and vegetables, and preparation and use thereof

A technology for postharvest diseases and marine yeast, which is applied in the fields of chemicals for biological control, botanical equipment and methods, biocides, etc., can solve the problems of unstable effect and high production cost, and achieve low cost and market prospects. Broad, economical and social benefits

Inactive Publication Date: 2009-04-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

It describes how different types of bacteria called Saccharomyces cerevisiae (Saccharomycetes) grow on plants that have specific requirements such as salt tolerance or water management. These organisms help prevent damage caused during storage and transportation processes while still being effective against harmful germs like mold spores. They work with other beneficial agents when they come into contact with them. Overall, this technology helps improve food safety and quality through reducing spoilage due to contamination.

Problems solved by technology

This patents discusses the problem of controlling or reducing harmful organism populations during processing and storing agriculturals like berries and tomatoes. Current methods involve adding agents called steroid compounds into crops, making it difficult to meet international standards set forth internations relating to safe consumption regulations. Therefore there needs further improvement measures to reduce these negative effects while maintaining beneficial levels within acceptable limits.

Method used

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  • Sea yeast for diseases biological control of postharvest fruits and vegetables, and preparation and use thereof
  • Sea yeast for diseases biological control of postharvest fruits and vegetables, and preparation and use thereof
  • Sea yeast for diseases biological control of postharvest fruits and vegetables, and preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1) Take dozens of seawater samples from the coastal waters of Zhoushan, Qingdao sea area, China, dilute them in different multiples, draw 0.1mL from the seawater samples of each dilution concentration and evenly spread them on the NYDA medium plate with a NaCl concentration of 2%. Place it upside down in an incubator and culture at a constant temperature of 28°C for 3 days. Use an inoculation loop to pick out a single colony with different culture characteristics for purification by streaking on a plate. After microscopic examination confirms that it is yeast, transfer it to the slant of NYDA medium for culture and storage.

[0033] 2) Inoculate the yeast preserved on the slant into a 250mL Erlenmeyer flask containing 70mL of sterilized NYDB medium, cultivate at a constant temperature of 28°C at 150 rpm for 18 hours, and centrifuge at 2000 rpm for 10 minutes to collect the bacteria. And wash the yeast cells twice with sterile water, count with a hemocytometer and adjust ...

Embodiment 2

[0037] 1) Take seawater samples from the coastal waters of Zhoushan and Qingdao sea areas in China, dilute them, draw 0.2mL of diluted seawater samples and spread them evenly on the plate of NYDA medium containing 2% NaCl, place them upside down in the incubator, and cultivate at a constant temperature of 26°C On the 4th day, use an inoculation loop to pick out a single colony with different cultural characteristics for purification by streaking on a plate. After microscopic examination confirms that it is yeast, it is inserted into the slant of NYDA medium for culture and storage;

[0038] 2) Inoculate the yeast preserved on the slant into a 250mL Erlenmeyer flask containing 35mL of sterilized NYDB medium, culture at a constant temperature of 26°C at 200 rpm for 30 hours, and centrifuge the fermentation broth at 3000 rpm for 5 minutes to collect bacteria body, and washed the yeast cells twice with sterile water, counted with a hemocytometer and adjusted to a concentration of 1...

Embodiment 3

[0047] Control effect of marine yeast strain R.paludigenum Fell & Tallman of the present invention on postharvest Alternaria and Botrytis cinerea diseases of cherry tomato fruit at room temperature

[0048] 1) Configuration of yeast cell suspension

[0049] Inoculate the preserved medium bacteria into a 250ml Erlenmeyer flask containing 35ml of sterilized medium after activation, culture at 150 rpm at 26°C for about 30 hours, and centrifuge at 2000 rpm for 10 minutes to collect the bacteria , and wash the yeast cells with sterile water, count with a hemocytometer, and adjust the concentration of the cell suspension to 10 8 CFU / mL of yeast cell suspension.

[0050] 2) In this embodiment, cherry tomatoes are used as test materials. The fruit comes from orchards in the suburbs of Hangzhou.

[0051] Pathogens: Alternaria alternata, Botrytis cinerea

[0052] Cherry tomato fruits were inoculated with yeast cell suspension after pricking. The inoculum size is 20 μl. After 2 hou...

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Abstract

The invention discloses a marine yeast for biologically preventing and treating postharvest diseases of fruits and vegetables, a method for preparing the same and application thereof. A marine yeast strain Rhodosporidium p aludigenum Fell & Tallman is screened out from the seawater of the coastal waters of the East China Sea. The colony of the marine yeast is pink, has a smooth surface, and grows in a culture medium added with 1 to 2 percent NaCl. The use of a cell suspension of the yeast with certain concentration can effectively inhibit various postharvest main mycoses such as alternaria, botrytis cinerea, penicilllum expansum, penicillium digitatum, and pestalotia. The marine yeast strain Rhodosporidium p aludigenum Fell & Tallman is used for biologically preventing and controlling the postharvest diseases of fruits and vegetables for the first time both at home and abroad. The strain has various functions of preventing decay, controlling oxidative stain, controlling softening flavour reversion and the like, and has the advantages of convenient use, no generation of antibiotic, no environment pollution, security, innocuity, and broad market prospect. At the same time, the strain is conducive to the export for earning foreign currency of China's high-quality fruit and vegetable products, and has significant economic and social benefits.

Description

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Claims

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Application Information

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Owner ZHEJIANG UNIV
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