Bacillus amyloliquefaciens subsp. plantarum and application thereof

A technology of amylolytic spores and bacillus plants, applied in the fields of application, plant growth regulators, botany equipment and methods, etc., can solve problems affecting product quality, health, harm to stems, pollution, etc., achieve good development and application prospects, and be easy to use The effect of industrialized production and simple cultivation conditions

Inactive Publication Date: 2012-10-03
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, strawberry anthracnose (Colletotrichum fragariae A.N.Brooks) is the most serious hazard. It damages stems, petioles, leaves, and stipules during the annual strawberry growth period, and damages flowers and fruits in the later stage, directly affecting strawberr

Method used

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  • Bacillus amyloliquefaciens subsp. plantarum and application thereof
  • Bacillus amyloliquefaciens subsp. plantarum and application thereof
  • Bacillus amyloliquefaciens subsp. plantarum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0038] Experimental example 1: Bacillus amyloliquefaciens subsp.plantarum (Bacillus amyloliquefaciens subsp.plantarum) B203 to strawberry anthracnose fungus Colletotrichum fragariae A.N.Brooks, strawberry gray mold Botrytis cinera Pers.ex Fr.), cacao two spores [Lasiodiplodia theobromae (Pat. ) Griffon & Maubl., Dothiorella gregaria Sacc., Phomopsis sp. and Apiocarpella macrospore (Speg.) H. & P.Syd.] Antibacterial effect.

[0039] The Bacillus amyloliquefaciens subsp.plantarum (Bacillus amyloliquefaciens subsp.plantarum) B203 bacterial strain activated with the LB slant was streak-inoculated on one side of the PDA plate culture medium in a 9cm glass petri dish, and then strawberry anthracnose, strawberry gray mold, For the PDA plate cultures of Dispora cocoa, Phomopsis poplaris, and Phomopsis poplaris, use a puncher with a diameter of 5 mm to get the same quality and same amount of bacterial blocks, and connect them to the other side of the plate. Cultivate at 28±1°C for 30 ...

experiment example 2

[0049] Experimental example 2: Bacillus amyloliquefaciens subsp.plantarum B203 metabolites on strawberry anthracnose bacteria Colletotrichum fragariae A.N.Brooks, strawberry gray mold Botrytis cinera Pers.ex Fr.), cacao bisporum [Lasiodiplodia theobromae ( Pat.) Griffon & Maubl., Dothiorella gregaria Sacc., Phomopsis sp. and Apiocarpella macrospore (Speg.) H. & P.Syd.] plate antibacterial effect.

[0050] The fermentation broth of Bacillus amyloliquefaciens subsp.plantarum cultivated for 3 days was filtered with a bacterial filter (the pore size of the filter membrane was 0.25μm); Punch out the same quality and the same amount of bacterial blocks, and inoculate one side of the PDA plate culture medium in a 9 cm diameter glass culture dish; then use a 5 mm diameter punch on the other side of the PDA plate to get the same quality and amount of Take out the round block and make a hole; put 100 μL of the filtered sterile filtrate of Bacillus amyloliquefaciens subsp. The biocontr...

experiment example 3

[0054] Experimental Example 3: A test to verify whether Bacillus amyloliquefaciens subsp. plantarum B203 has an inhibitory effect on strawberry anthracnose.

[0055] 1. Experimental equipment: incubator, shaker, ultra-clean workbench;

[0056] 2. Experimental principle:

[0057] Before strawberry cultivation, put strawberry roots in the culture solution of Bacillus amyloliquefaciens subsp.plantarum (Bacillus amyloliquefaciens subsp.plantarum) B203. It can adhere to the strawberry root to continue to grow and produce metabolites, thereby inhibiting the growth of strawberry anthracnose.

[0058] 3. Experimental method:

[0059] 1) Cultivation of Bacillus amyloliquefaciens subsp.plantarum B203 bacterial liquid: Inoculate Bacillus amyloliquefaciens subsp.plantarum B203 into an Erlenmeyer flask containing 50 mL of LB medium , 120 rpm, and cultured at 35°C for 60 hours.

[0060] 2) Preparation of bacteria-carrying strawberry roots: Put the strawberry seedlings to be cultivated i...

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Abstract

The invention discloses Bacillus amyloliquefaciens subsp. plantarum. The classified nomenclature of the Bacillus amyloliquefaciens subsp. plantarum is Bacillus amyloliquefaciens subsp. plantarum B203; the Bacillus amyloliquefaciens subsp. plantarum was collected in China General Microbiological Culture Collection Center on May 25, 2012; and the collection number is CGMCC No. 6156. The Bacillus amyloliquefaciens subsp. plantarum is mainly used for biological control of strawberry anthracnose. The Bacillus amyloliquefaciens subsp. plantarum B203 has a remarkable inhibitory effect on Colletotrichum fragaliae Brooks, strawberry Botrytis cinerea and various pathogenic fungi of poplar canker, and the antagonistic bacterium also has a good control effect on various other plant diseases; the propagation speed is high; the Bacillus amyloliquefaciens subsp. plantarum can be manually cultured, is easy to operate, and can be conveniently produced and applied; the Bacillus amyloliquefaciens subsp. plantarum has the advantages of high stress resistance and the like, and has an important significance for controlling the strawberry anthracnose caused by fungi; and culture conditions are simple, and the Bacillus amyloliquefaciens subsp. plantarum is easy to preserve, can be industrially produced easily, and has a good development and application prospect.

Description

technical field [0001] The invention belongs to the field of plant disease prevention and control, and in particular relates to a plant subspecies of Bacillus amyloliquefaciens and its application in the prevention and treatment of strawberry anthracnose. Background technique [0002] In recent years, with the general concern of the public on the safety of chemical pesticides in food and the environment, and the prohibition of some dangerous chemical pesticides, people are seeking new ways to control plant diseases safely and effectively, and the use of beneficial microorganisms to control plant diseases has attracted increasing attention. It introduces exogenous antagonistic bacteria that are beneficial to plants into the broken plant natural ecosystem, or promotes the growth of natural beneficial microbial populations by regulating the environment, and exhibits biological control activity, and achieves disease prevention through the interaction between microorganisms Purpo...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00C12R1/07
Inventor 赵桂华席刚俊史俊徐超杨鹤同
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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