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Bacillus atrophaeus and application thereof

A technology of Bacillus spp. and Bacillus, applied in the biological field, can solve problems such as food safety hazards, environmental and ecological balance pressures, etc.

Active Publication Date: 2015-09-30
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the long-term and extensive use of chemical pesticides not only leads to food safety hazards caused by drug residues, but also poses a huge pressure on the environment and ecological balance

Method used

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  • Bacillus atrophaeus and application thereof
  • Bacillus atrophaeus and application thereof
  • Bacillus atrophaeus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] Embodiment 1, the isolation and identification of Bacillus atrophaeus (Bacillus atrophaeus) JZB120050CGMCC No.10919

[0107] 1. Isolation of Bacillus atrophaeus JZB120050CGMCC No.10919

[0108] The following operations were performed on 50 soil samples collected from Inner Mongolia: Take 1 g of soil samples, add 9 mL of sterile water, shake for 15 minutes to fully dissolve, mix well, and treat them in a high-temperature water bath in a boiling water bath for 10 minutes, during which time shake for 2-3 Second-rate. After shaking, draw 1mL stock solution and add it into a test tube filled with 9mL sterile water to obtain 10 ﹣1 1-fold dilution, and then serially diluted to obtain a dilution factor of 10 ﹣2 -10 ﹣6 Pipette 100 μL of different dilutions on the CM0002 medium plate, spread it evenly with a spreader, mark it, and repeat each gradient 3 times; then place the coated plate upside down and place it at a constant temperature of 37°C Incubate overnight in the box....

Embodiment 2

[0126] Embodiment 2, the mensuration of the bacteriostatic spectrum of Bacillus atrophaeus (Bacillus atrophaeus) JZB120050CGMCC No.10919

[0127] Pathogens tested:

[0128] Botrytis cinerea Per.ex Fr. Botrytis cinerea Per.ex Fr.

[0129] Strawberry gray mold (Botrytis cinerea Persoon);

[0130] Fusarium oxysporum Schl.f.sp.conglutinans (Wollenw.) Snyder&Hansen];

[0131] Fusarium oxysporum f.sp.niveum;

[0132] Fusarium wilt of cotton - Fusarium oxysporum Schl.f.sp.vasinfectum (Atk) Snyder&Hansen];

[0133] Pea Fusarium solani root rot - Fusarium solani f.sp.pisi;

[0134] Lily root rot fungus - Fusarium oxysporum Schlecht.;

[0135] Wheat scab - Fusarium graminearum Schwabe;

[0136] Wheat sheath blight (Rhizoctonia cerealis);

[0137] Wheat take-all pathogen [Gaeumannomyces graminis (Sacc.) ArX & Olivier var. tritici J. Walker];

[0138] Peach brown rot fungus-strep sclerotinia [Monilinia fructicola (wint.) Rehm];

[0139] Grape anthracnose - Colletortrichum gloeosp...

Embodiment 3

[0153] Example 3, Bacillus atrophaeus (Bacillus atrophaeus) JZB120050CGMCC No.10919 biological control-related functional detection

[0154] 1. Determination of Biofilm Formation Ability

[0155] 1. Strain culture: Adopt polypropylene tube microplate culture method, inoculate a ring of seed solution of JZB120050 strain that has been activated for 16-18 hours into 5 mL of LB culture solution, and culture with shaking at 30°C overnight to obtain the bacterial solution of JZB120050 strain. Inoculate 10 μL of JZB120050 strain bacterial solution into a polypropylene tube filled with 0.5 mL CM culture solution, and incubate statically at 30°C for 12-24 hours.

[0156] 2. Biofilm detection: use crystal violet staining method, pour out the culture medium after the culture is terminated, wash with deionized water, stain the adherent cells with 1% crystal violet for 2 minutes, wash with water, observe the formation of biofilm, according to the following grading standards Statistics: Gr...

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Abstract

The invention discloses Bacillus atrophaeus and an application thereof. The Bacillus atrophaeus JZB120050CGMCC No.10919 has stable, efficient and broad-spectrum antibacterial properties. The Bacillus atrophaeus can generate a biological membrane, has strong capability of generating siderophore, chitinase, cellulase and protease, which shows that the strain has relatively strong biocontrol potential. The control effect of fermentation liquor of the strain to tomatoes inoculated with botrytis cinerea for culturing 11d is 100% (the control effect of a 5-time diluent treatment group of JZB120050 strain fermentation liquor is 48%, the control effect of a treatment group of JZB120028 strain fermentation liquor is 50%, the control effect of a 5-time diluent treatment group of JZB120028 strain fermentation liquor is 26%, and the control effects of a blank control group, a 1000-time liquid treatment group of 3% polyoxin, and 1000-time liquid treatment group of 50% carbendazim are 0%), which shows that the strain has an effective inhibitory action on the botrytis cinerea. The strain is safe to human and domestic animals, does not have environmental pollution problems, is simple in culture condition, easy to store and suitable for industrial production, and has a good development application prospect.

Description

technical field [0001] The invention relates to a strain of bacillus fueci and its application in the field of biotechnology. Background technique [0002] Plant fungal diseases caused by phytopathogenic fungi pose a huge threat to agricultural production and income. For a long time, selecting disease-resistant varieties and applying chemical pesticides have become the first choice for the prevention and treatment of fungal diseases. As an important means of production in agriculture, pesticides have a great impact on food safety production. Chemical pesticides are the most important plant protection inputs in crop disease control, and they have played a huge role in disease control. However, the long-term and large-scale use of chemical pesticides not only leads to food safety hazards caused by drug residues, but also poses a huge pressure on the environment and ecological balance. Compared with chemical pesticides, biological pesticides have the characteristics of long-la...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
Inventor 卢彩鸽刘伟成张殿朋刘德文刘霆吴慧玲卢向阳董丹张涛涛田兆丰
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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