Chinese herbal medicine leavening, preparation method thereof and application of Chinese herbal medicine leavening in cosmetics

Abstract

The invention discloses a Chinese herbal medicine leavening, a preparation method thereof and application of the Chinese herbal medicine leavening in cosmetics, and particularly relates to the technical field of cosmetics. Comprising the following raw materials in parts by weight: 12-18 parts of radix astragali, 8-12 parts of radix ampelopsis, 10-15 parts of radix saposhnikoviae, 18-22 parts of rhizoma chuanxiong, 6-9 parts of bitter almond seeds, 18-22 parts of radix salviae miltiorrhizae, 7-10 parts of herba centellae, 6-9 parts of folium artemisiae argyi, 7-10 parts of fructus gardeniae, 4-6 parts of flos sophorae immaturus, 5-8 parts of fructus citri, 12-15 parts of poria cocos and 1.5-2.5 parts of a dendrobium officinale extract. The composition can synergistically exert moisturizing, immune regulation and anti-allergy effects with other raw material components to form an anti-allergy, anti-oxidation and repair efficacy closed loop, a process chain from aspergillus oryzae high-enzyme-activity solid fermentation to double-bacterium liquid symbiosis to chitinase directional enzymolysis can synergistically improve the yield of active matters, and the situation that traditional anti-allergy components have no hair growth effect is broken through, so that the anti-allergy and anti-allergy effects are achieved. Due to the limitation that the hair restorer stimulates sensitive skin, the hair growing and double effects are integrated, and the hair restorer is suitable for preparing cosmetics for resisting allergy, resisting oxidation and repairing barriers and is especially suitable for sensitive skin care and scalp care.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and more particularly to a Chinese herbal medicine fermentation product, a preparation method thereof, and application thereof in cosmetics. Background Art

[0002] The application of Chinese herbal fermentation products in cosmetics has undergone an iteration from traditional experience to modern technology, forming a multi-dimensional technological progress. There have been records of Chinese herbal skin care in ancient books, such as the "Zi Bu" in the Siku Quanshu, which removes powder residue, chapped skin and hair, making the face smooth and shiny like when it was fourteen or fifteen years old. The recipe itself is a mixture of various medicinal materials and eggs, which is applied to the face and washed with water after one night. It can be understood as an ancient version of a ready-to-mix, smeared night mask, in which the main medicinal materials include astragalus (Huangqi), Atractylodes macrocephala, Bletilla striata, Eagle Shit White, Saposhnikovia divaricata, Angelica dahurica, Aconitum carmichaelii, Chuanxiong (i.e. Ligusticum chuanxiong), apricot kernel, etc. The recipe can be traced back to the ninth facial medicine in Sun Simiao's "Prescriptions for Emergencies" in the Tang Dynasty. After being handed down to the Qing Dynasty, it was included in the Siku Quanshu again, proving that its effect is relatively real and reliable. This type of traditional formula can achieve basic cleansing and nourishment through ready-to-mix use, but is limited by extraction technology, and the utilization rate of active ingredients is less than 10%. Currently, sensitive skin care and scalp health issues are receiving increasing attention, and the market is in urgent need of natural ingredients that have anti-allergic, repairing and specific effects such as hair growth.

[0003] On the one hand, although traditional anti-allergic ingredients such as dipotassium glycyrrhizate and purslane extract can relieve skin sensitivity, they lack the function of regulating hair follicles and cannot improve scalp hair loss problems. On the other hand, although mainstream hair growth agents such as minoxidil and capsicum tincture can promote hair growth, they are highly irritating and can easily cause redness, swelling and itching on sensitive skin, and are especially unsuitable for people with fragile scalp barriers. The split in the efficacy of the two makes it difficult for people with sensitive skin to obtain both anti-allergic care and hair growth improvement at the same time. The market lacks dual-action ingredients that can both mildly combat allergies, repair the barrier, and safely promote hair growth. At the same time, the existing fermentation process has not formed a synergistic system of high enzyme activity solid-state fermentation, dual-bacteria liquid symbiosis and directed enzymatic hydrolysis, resulting in low yield of active ingredients and poor stability, further limiting its application in sensitive skin and scalp care. Summary of the Invention

[0004] In order to overcome the above-mentioned defects of the prior art, the present invention provides a Chinese herbal medicine fermentation product, a preparation method thereof, and application thereof in cosmetics, so as to solve the problems raised in the above-mentioned background technology.

[0005] To achieve the above object, the present invention provides the following technical solution: a Chinese herbal medicine fermentation product, comprising the following raw materials in parts by weight: 12-18 parts of astragalus, 8-12 parts of white ampelopsis, 10-15 parts of siler, 18-22 parts of ligusticum chuanxiong, 6-9 parts of amygdalus seeds, 18-22 parts of salvia miltiorrhiza, 7-10 parts of centella asiatica, 6-9 parts of wormwood, 7-10 parts of gardenia, 4-6 parts of sophora japonica seeds, 5-8 parts of citron, 12-15 parts of poria, and 1.5-2.5 parts of dendrobium officinale extract; The preparation of the Chinese herbal medicine fermentation product comprises the following steps: S1: Grind all raw materials into 80-100 mesh, mix the raw materials with water in a ratio of 1:6-1:9, and sequentially treat with a 500-700W microwave for 3-4 minutes, followed by ultrasonic wall breaking at 450-550W for 15-20 minutes; S2: Inoculate Aspergillus oryzae, add 0.2-0.4 M citric acid to adjust the pH to 4.2-4.8, and solid-state ferment at 32-34°C for 36-48 hours; S3: After sterilization, a mixture of Lactobacillus plantarum and Aureobasidium pullulans is inoculated at an inoculum concentration of 6-9% and fermented in liquid form at 26-28°C for 24-36 hours. The temperature is then raised to 36-38°C, 0.3-0.5% chitinase is added, and fermentation continues for 15-20 hours. S4: After the fermentation broth is filtered through a 200 nm ceramic membrane and decolorized through a 10 kDa ultrafiltration membrane, it is mixed with a composite solution of 0.8-1.2% sodium alginate and 0.5-0.7% chitosan and spray-dried at an inlet air temperature of 160-170°C and an outlet air temperature of 80-85°C to obtain a microencapsulated freeze-dried powder with a particle size of 3-6 μm.

[0006] Preferably, the Dendrobium officinale extract is a polysaccharide component with a molecular weight of ≤5 kDa, and the polysaccharide purity is ≥90%.

[0007] Preferably, the Aspergillus oryzae in S2 is deposited under the accession number CICC 41457, and its cellulase activity during solid-state fermentation is ≥150 U / g, and the inoculation amount is 10 6 -10 7 CFU / g dry basis material.

[0008] Preferably, the mass ratio of sodium alginate to chitosan in the composite microcapsule in S4 is 1.5:1.

[0009] Preferably, the inoculation amount of the mixed strains in S3 is 8%, the fermentation temperature is 27°C, the volume ratio of the mixed strains in S3 is 1:1, the concentration is 10 7 -10 8 CFU / mL.

[0010] Preferably, the fermentation product has a total polyphenol content of ≥7.2 mg / g, a polysaccharide content of ≥11 mg / g, a salvianolic acid B content of ≥0.7 mg / g, and an asiaticoside content of ≥1.5 mg / g.

[0011] The invention relates to the application of the above-mentioned Chinese herbal fermentation products in the preparation of cosmetics with anti-allergic, antioxidant and barrier repair effects.

[0012] Preferably, when the cosmetic is used for sensitive skin care, the fermentation product is added in an amount of 1.5%-4%, and the efficacy is achieved by inhibiting hyaluronidase activity with an inhibition rate of ≥55% and scavenging DPPH free radicals with a scavenging rate of ≥85%.

[0013] Preferably, when the cosmetic is used for scalp care, the fermentation product is added in an amount of 2%-6%, which promotes hair growth by increasing the IGF-1 level of hair follicles by ≥20%.

[0014] Technical effects and advantages of the present invention: 1. The formula uses a variety of traditional Chinese herbal medicines such as Astragalus, Bletilla striata, Saposhnikovia divaricata, Centella asiatica, and Salvia miltiorrhiza. Each ingredient has potential for anti-allergic, antioxidant, and skin or scalp barrier repair. Centella asiatica promotes repair, Astragalus is an antioxidant, and Saposhnikovia divaricata is anti-allergic. The synergistic effect can enhance the overall efficacy. Paired with high-purity Dendrobium officinale polysaccharides, the low-molecular-weight polysaccharide has high bioavailability and can synergize with other raw materials to exert moisturizing, immunomodulatory, and anti-allergic effects, forming a closed loop of anti-allergic, antioxidant, and repair effects. The process chain, which involves solid-state fermentation with high enzyme activity of Aspergillus oryzae, dual-bacteria liquid symbiosis, and then chitinase-directed enzymatic hydrolysis, can synergistically improve the yield of active ingredients, breaking the limitations of traditional anti-allergic ingredients that have no hair growth effect and hair growth agents that irritate sensitive skin. By regulating the immune microenvironment of hair follicles through small-molecule polysaccharides from Dendrobium officinale, the dual-effect hair growth and hair growth effect are achieved in one. 2. In the pretreatment stage, microwaves are used to promote the rapid dissolution of raw materials, and ultrasonic waves are used to break down cell structures, significantly improving the extraction rate of active ingredients such as polyphenols, polysaccharides, and asiaticoside. Solid-state fermentation with Aspergillus oryzae decomposes macromolecules such as cellulose in the raw materials, releasing more active substances. Liquid fermentation with a mixture of Lactobacillus plantarum and Aureobasidium pullulans produces metabolites such as organic acids and active peptides, enhancing anti-allergic and anti-inflammatory effects. Chitinase is subsequently added to further decompose the ingredients and improve the utilization rate of active substances. Ceramic membranes and ultrafiltration membranes are used to remove impurities and improve purity. Microencapsulation can protect the active ingredients from external environmental influences and improve stability. The 3-6μm microcapsule particle size and freeze-dried powder form facilitate rapid absorption by the skin or scalp, enhancing bioavailability. 3. The minimum content of total polyphenols, polysaccharides, salvianolic acid B and asiaticoside in the fermentation product is clearly defined. Total polyphenols have strong antioxidant properties, polysaccharides moisturize and repair the skin barrier, salvianolic acid B is anti-inflammatory and antioxidant, and asiaticoside promotes skin repair. The controllable content ensures the stability and reliability of the product's efficacy. It is suitable for the preparation of cosmetics with anti-allergic, antioxidant and barrier-repairing effects, especially for sensitive skin care and scalp care. Its suitability for sensitive skin reflects its mild anti-allergic properties, while scalp care specifically repairs the scalp barrier. The clear range of added amounts ensures efficacy while reducing the risk of irritation, achieving a balance between safety and effectiveness. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the method flow structure of the present invention. DETAILED DESCRIPTION

[0016] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0017] Example 1 Raw material ratio (parts by weight): 15 parts astragalus, 10 parts white ampelopsis, 12 parts siler, 20 parts ligusticum, 8 parts amygdalus seed, 20 parts salvia miltiorrhiza, 8 parts centella asiatica, 7 parts wormwood, 8 parts gardenia, 5 parts sophora japonica, 6 parts citron, 14 parts poria, 2 parts dendrobium officinale extract (molecular weight ≤ 5kDa, purity 92%); Preparation steps of Chinese herbal fermentation products: S1 preprocessing: The raw materials were crushed to 80 mesh, and water was added and mixed at a material-liquid ratio of 1:7; The cells were treated with a 600W microwave for 3.5 min and then ultrasonically broken for 18 min.

[0018] S2 solid-state fermentation: Inoculate Aspergillus oryzae CICC41457 (1.5×10 6 CFU / g dry material, determined according to GB / T4789.2-2022 "National Food Safety Standard for Microbiological Examination of Foods, Determination of Total Colony Count"), add 0.3M citric acid to adjust the pH to 4.5; Fermentation at 33°C for 42 h (cellulase activity 158 U / g).

[0019] S3 liquid fermentation: After sterilization, Lactobacillus plantarum CGMCC1.595 and Aureobasidium pullulans CICC40348 were inoculated (volume ratio 1:1, total inoculation volume 8%, bacterial concentration 5×10 7CFU / mL); Ferment at 27°C for 30 h, then raise the temperature to 37°C, add 0.4% chitinase and continue fermentation for 18 h.

[0020] S4 microencapsulation: The fermentation broth was filtered through a 200 nm ceramic membrane and decolorized with a 10 kDa ultrafiltration membrane; mixed with 1.0% sodium alginate and 0.67% chitosan (mass ratio 1.5:1); Spray drying (inlet air 165°C, outlet air 82°C) was performed to obtain a freeze-dried powder with a particle size of 4.2±0.5 μm.

[0021] Example 2 Raw material ratio (parts by weight): 12 parts astragalus, 8 parts white ampelopsis, 10 parts siler, 18 parts ligusticum, 18 parts chuanxiong, 6 parts amygdalus seed, 18 parts salvia miltiorrhiza, 7 parts centella asiatica, 6 parts artemisia argyi, 7 parts gardenia, 4 parts sophora japonica, 5 parts citron, 12 parts poria, 1.5 parts dendrobium officinale extract (molecular weight ≤ 5kDa, purity 92%).

[0022] Preparation steps of Chinese herbal fermentation products: S1 preprocessing: The raw materials were crushed to 80 mesh, and water was added and mixed at a material-liquid ratio of 1:7; The cells were treated with a 600W microwave for 3.5 min and then ultrasonically broken for 18 min.

[0023] S2 solid-state fermentation: Inoculate Aspergillus oryzae CICC41457 (1.5×10 6 CFU / g dry material, determined according to GB / T4789.2-2022 "National Food Safety Standard for Microbiological Examination of Foods, Determination of Total Colony Count"), add 0.3M citric acid to adjust the pH to 4.5; Fermentation at 33°C for 42 h (cellulase activity 158 U / g).

[0024] S3 liquid fermentation: After sterilization, Lactobacillus plantarum CGMCC1.595 and Aureobasidium pullulans CICC40348 were inoculated (volume ratio 1:1, total inoculation volume 8%, bacterial concentration 5×10 7 CFU / mL); Ferment at 27°C for 30 h, then raise the temperature to 37°C, add 0.4% chitinase and continue fermentation for 18 h.

[0025] S4 microencapsulation: The fermentation broth was filtered through a 200 nm ceramic membrane and decolorized with a 10 kDa ultrafiltration membrane; mixed with 1.0% sodium alginate and 0.67% chitosan (mass ratio 1.5:1); Spray drying (inlet air 165°C, outlet air 82°C) was performed to obtain a freeze-dried powder with a particle size of 4.2±0.5 μm.

[0026] Example 3 Raw material ratio (weight parts): Astragalus 18, Bletilla striata 12, Saposhnikovia divaricata 15, Chuanxiong 22, Prunus amygdalus dulcis seed 9, Salvia miltiorrhiza 22, Centella asiatica 10, Artemisia argyi 9, Gardenia jasminoides 10, Sophora japonica 6, Citron 8, Poria cocos 15, Dendrobium officinale extract 2.5 (molecular weight ≤ 5kDa, purity 92%).

[0027] Preparation steps of Chinese herbal fermentation products: S1 preprocessing: The raw materials were crushed to 80 mesh, and water was added and mixed at a material-liquid ratio of 1:7; The cells were treated with a 600W microwave for 3.5 min and then ultrasonically broken for 18 min.

[0028] S2 solid-state fermentation: Inoculate Aspergillus oryzae CICC41457 (1.5×10 6 CFU / g dry material, determined according to GB / T4789.2-2022 "National Food Safety Standard for Microbiological Examination of Foods, Determination of Total Colony Count"), add 0.3M citric acid to adjust the pH to 4.5; Fermentation at 33°C for 42 h (cellulase activity 158 U / g).

[0029] S3 liquid fermentation: After sterilization, Lactobacillus plantarum CGMCC1.595 and Aureobasidium pullulans CICC40348 were inoculated (volume ratio 1:1, total inoculation volume 8%, bacterial concentration 5×10 7 CFU / mL); Ferment at 27°C for 30 h, then raise the temperature to 37°C, add 0.4% chitinase and continue fermentation for 18 h.

[0030] S4 microencapsulation: The fermentation broth was filtered through a 200 nm ceramic membrane and decolorized with a 10 kDa ultrafiltration membrane; mixed with 1.0% sodium alginate and 0.67% chitosan (mass ratio 1.5:1); Spray drying (inlet air 165°C, outlet air 82°C) was performed to obtain a freeze-dried powder with a particle size of 4.2±0.5 μm.

[0031] According to the final component content detection of the Chinese herbal medicine fermentation product of Examples 1-3: Ingredients Detection method Example 1 Example 2 Example 3 Total polyphenol Folin-phenol method in Chinese Pharmacopoeia 2020 edition 7.8±0.3mg / g 7.3±0.2mg / g 8.1±0.4mg / g Polysaccharide Phenol-sulfuric acid method in GB / T31742-2015 12.5±0.6mg / g 11.2±0.5mg / g 13.7±0.7mg / g Danshensuan B HPLC method in Chinese Pharmacopoeia 2020 edition 0.75±0.05mg / g 0.71±0.04mg / g 0.79±0.06mg / g The efficacy of cosmetics was verified by using the fermented Chinese herbal medicine in cosmetics according to Example 1 (vs. no treatment): (1) Sensitive skin care (3% added) Test item Method standard Result Hyaluronidase inhibition rate Cosmetic Safety Technical Specifications 2022 edition 74.3±2.5% DPPH free radical scavenging rate GB / T39199-2020 91.6±1.8% Skin barrier repair (TEWL↓) Corneometer 28.7% improvement (2) Scalp care (5% added) Test item Method Result Hair follicle IGF-1 promotion level Human hair follicle in vitro culture + ELISA method 24.5±3.2%↑ Hair growth period extension Hair follicle microscopic observation (Anagen / Telogen ratio) 35.1% extension In summary, during the preparation of Chinese herbal fermentation products, the ratio of Chuanxiong and Danshen (18-22 parts) increased the yield of salvianolic acid B to 1.8 times that of the traditional process, reflecting the synergistic effect of the components. The three-step fermentation increased the total amount of active ingredients. The stability of 1.5:1 sodium alginate / chitosan microcapsules increased by 2.3 times in an accelerated test at 45°C. The 3% addition achieved a DPPH scavenging rate of >91% (the national standard requires ≥80% for high-efficiency antioxidants). The 5% addition increased hair follicle IGF-1 by ≥24.5%.

[0032] Finally, it should be noted that the drawings of the embodiments disclosed herein only relate to structures related to the embodiments disclosed herein, and other structures may refer to conventional designs. In the absence of conflicts, the same embodiment and different embodiments of the present invention may be combined with each other. Finally: The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A fermented Chinese herbal medicine, characterized in that: The invention is composed of the following raw materials in parts by weight: 12-18 parts of astragalus, 8-12 parts of white ampelopsis, 10-15 parts of siler, 18-22 parts of ligusticum chuanxiong, 6-9 parts of amygdalus seeds, 18-22 parts of salvia miltiorrhiza, 7-10 parts of centella asiatica, 6-9 parts of wormwood, 7-10 parts of gardenia, 4-6 parts of sophora japonica seeds, 5-8 parts of citron, 12-15 parts of poria, and 1.5-2.5 parts of dendrobium officinale extract; The preparation of the Chinese herbal medicine fermentation product comprises the following steps: S1: Grind all raw materials into 80-100 mesh, mix the raw materials with water in a ratio of 1:6-1:9, and sequentially treat with a 500-700W microwave for 3-4 minutes, followed by ultrasonic wall breaking at 450-550W for 15-20 minutes; S2: Inoculate Aspergillus oryzae, add 0.2-0.4 M citric acid to adjust the pH to 4.2-4.8, and solid-state ferment at 32-34°C for 36-48 hours; S3: After sterilization, a mixture of Lactobacillus plantarum and Aureobasidium pullulans was inoculated at an inoculum concentration of 6-9%. The mixture was fermented in a liquid state at 26-28°C for 24-36 hours. The temperature was then raised to 36-38°C, 0.3-0.5% chitinase was added, and the fermentation was continued for 15-20 hours. S4: After the fermentation broth is filtered through a 200 nm ceramic membrane and decolorized through a 10 kDa ultrafiltration membrane, it is mixed with a composite solution of 0.8-1.2% sodium alginate and 0.5-0.7% chitosan and spray-dried at an inlet air temperature of 160-170°C and an outlet air temperature of 80-85°C to obtain a microencapsulated freeze-dried powder with a particle size of 3-6 μm.

2. The Chinese herbal medicine fermentation product according to claim 1, characterized in that: The Dendrobium officinale extract is a polysaccharide component with a molecular weight of ≤5kDa, and the polysaccharide purity is ≥90%.

3. The Chinese herbal medicine fermentation product according to claim 1, characterized in that: The Aspergillus oryzae in S2 has a preservation number of CICC 41457, and its cellulase activity during solid-state fermentation is ≥150 U / g, and the inoculation amount is 10 6 -10 7 CFU / g dry basis material.

4. The Chinese herbal medicine fermentation product according to claim 1, characterized in that: The mass ratio of sodium alginate to chitosan in the composite microcapsule in S4 is 1.5:

1.

5. The Chinese herbal medicine fermentation product according to claim 1, characterized in that: The inoculation amount of the mixed strains in S3 is 8%, the fermentation temperature is 27°C, the volume ratio of the mixed strains in S3 is 1:1, the concentration is 10 7 -10 8 CFU / mL.

6. The Chinese herbal medicine fermentation product according to claim 1, characterized in that: The fermented product has a total polyphenol content of ≥7.2 mg / g, a polysaccharide content of ≥11 mg / g, a salvianolic acid B content of ≥0.7 mg / g, and an asiaticoside content of ≥1.5 mg / g.

7. Use of the Chinese herbal fermentation product according to any one of claims 1 to 6 in the preparation of cosmetics with anti-allergic, antioxidant and barrier repair effects.

8. The use according to claim 7, characterized in that: When the cosmetic is used for sensitive skin care, the amount of fermented product added is 1.5%-4%.

9. The use according to claim 7, characterized in that: When the cosmetic is used for scalp care, the fermented product is added in an amount of 2% to 6%.

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