HPLC-ELSD (high performance liquid chromatography-evaporation light-scattering detection) measuring method of grape berry sugar

A determination method, glucose technology, applied in the direction of measuring devices, material separation, analysis materials, etc., can solve the problems of chemical degradation affecting column efficiency, incomplete separation of target peaks, high acetonitrile purity requirements, etc., to reduce separation time and strong practicability , The effect of complete sugar extraction

Inactive Publication Date: 2013-08-28
SICHUAN AGRI UNIV
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

When the traditional high performance liquid chromatography is used to determine sugar, the amino column in the commonly used chromatographic column has high requirements for the purity of acetonitrile, poor stability, easy loss of stationary phase, short life, and high price.
ODS column (octadecylsilane bonded silica filler, also known as C18 c

Method used

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  • HPLC-ELSD (high performance liquid chromatography-evaporation light-scattering detection) measuring method of grape berry sugar
  • HPLC-ELSD (high performance liquid chromatography-evaporation light-scattering detection) measuring method of grape berry sugar
  • HPLC-ELSD (high performance liquid chromatography-evaporation light-scattering detection) measuring method of grape berry sugar

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Experimental program
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Example Embodiment

[0031] Example 1:

[0032] (1) Materials

[0033] Early-maturing grape variety'Xiahei', late-maturing grape variety'Red Earth' and'Beirenzhi', cultivated in shelter from rain in Pengshan County, Meishan City, Sichuan Province, and late-maturing grape variety'Red Earth' cultivated in open field.

[0034] (2) Method

[0035] 1) Sampling

[0036] 20-25 days after the flowering of grapes (the sampling time of early-maturing variety'Xiahei' is 20 days after flowering, and the sampling time of late-maturing varieties'Red Globe' and'Beirenzhi' is 25 days after flowering), in the east, west, and west of the plant In the four directions of south and north and the upper, middle and lower positions of the ears, pick medium-sized, disease-free fruits every 7-10 days (the sampling interval of the early-maturing variety'Xiahei' is 7 days, and the late-maturing variety'Red Earth' , The sampling interval of'Beauty Finger' is 10d) Take a sample; immediately put the fruit in the ice box after picking, ...

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Abstract

The invention discloses an HPLC-ELSD (high performance liquid chromatography-evaporation light-scattering detection) measuring method of grape berry sugar, belonging to the technical field of food sugar detection. The method comprises the following steps: firstly, juicing and homogenizing grapes, ultrasonic extracting the juice, and then, centrifuging the juice, taking the supernatant, filtering the supernatant and using the filtrate as a test sample; respectively preparing standard working solutions with a series of concentrations of glucose, fructose and sucrose; the chromatographic conditions are as follows: a polystyrene-divinyl benzene bonded amino sugar column serves as a chromatographic column, the mobile phase is a mixture of acetonitrile and water with volume ratio of 70-80:30-20, and the flow velocity is 1mL/min, the column temperature is 30-35 DEG C, the detector is ELSD, the temperature of a detecting pool is 40-80 DEG C, and the sample size is 10 microliters; and then inspecting linear dependence and precision, and detecting stability and sample recovery. The method provided by the invention is simple and convenient to operate, small in time consumption, good in measurement result repeatability, high in precision and capable of accurately and quickly measuring the contents of glucose, fructose and sucrose in grape berry.

Description

technical field [0001] The invention belongs to the technical field of food sugar detection, in particular to an HPLC-ELSD determination method for grape sugar. Background technique [0002] Grapes are one of the most important fruits in the world. Grape fruit is thin and juicy, sweet and sour, and rich in nutrition. The sugar content in ripe berries can reach 10% to 30%. The sugar content of grape fruit is mainly glucose and fructose, and also contains a small amount of sucrose, which is easily absorbed by the human body; Digestion: Grape fruit contains a variety of beneficial minerals, vitamins, amino acids, lecithin, and anthocyanins, flavonoids, resveratrol and other ingredients that are beneficial to the human body. Grapes are widely used and can be used for fresh consumption, wine making, juice making and drying. The sugar content of grape fruit is an important index affecting the quality of berries. The composition and content of sugar not only directly affect the...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/14
Inventor 邓群仙李梦鸽董礼吕秀兰廖明安刘健程雪丽李康宁
Owner SICHUAN AGRI UNIV
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