HPLC-ELSD (high performance liquid chromatography-evaporation light-scattering detection) measuring method of grape berry sugar

Abstract

The invention discloses an HPLC-ELSD (high performance liquid chromatography-evaporation light-scattering detection) measuring method of grape berry sugar, belonging to the technical field of food sugar detection. The method comprises the following steps: firstly, juicing and homogenizing grapes, ultrasonic extracting the juice, and then, centrifuging the juice, taking the supernatant, filtering the supernatant and using the filtrate as a test sample; respectively preparing standard working solutions with a series of concentrations of glucose, fructose and sucrose; the chromatographic conditions are as follows: a polystyrene-divinyl benzene bonded amino sugar column serves as a chromatographic column, the mobile phase is a mixture of acetonitrile and water with volume ratio of 70-80:30-20, and the flow velocity is 1mL / min, the column temperature is 30-35 DEG C, the detector is ELSD, the temperature of a detecting pool is 40-80 DEG C, and the sample size is 10 microliters; and then inspecting linear dependence and precision, and detecting stability and sample recovery. The method provided by the invention is simple and convenient to operate, small in time consumption, good in measurement result repeatability, high in precision and capable of accurately and quickly measuring the contents of glucose, fructose and sucrose in grape berry.

Description

technical field [0001] The invention belongs to the technical field of food sugar detection, in particular to an HPLC-ELSD determination method for grape sugar. Background technique [0002] Grapes are one of the most important fruits in the world. Grape fruit is thin and juicy, sweet and sour, and rich in nutrition. The sugar content in ripe berries can reach 10% to 30%. The sugar content of grape fruit is mainly glucose and fructose, and also contains a small amount of sucrose, which is easily absorbed by the human body; Digestion: Grape fruit contains a variety of beneficial minerals, vitamins, amino acids, lecithin, and anthocyanins, flavonoids, resveratrol and other ingredients that are beneficial to the human body. Grapes are widely used and can be used for fresh consumption, wine making, juice making and drying. The sugar content of grape fruit is an important index affecting the quality of berries. The composition and content of sugar not only directly affect the...

AI Technical Summary

Problems solved by technology

When the traditional high performance liquid chromatography is used to determine sugar, the amino column in the commonly used chromatographic column has high requirements for the purity of acetonitrile, poor stability, easy loss of stationary phase, short life, and high price.

ODS column (octadecylsilane bonded silica filler, also known as C18 column) has incomplete separation of target peaks and unsatisfactory results

Waters high-efficiency sugar column, Zorbaxa high-efficiency sugar column, etc. all have isocratic elution. The separation of some sugars is not as good as gradient elution. If gradient elution may cause chemical degradation of the column bed and affect column efficiency

Images