The invention relates to the technical field of thermal transfer printing lettering film, and in particular, relates to a high-elasticity silicone rubber lettering film and a preparation method and anapplication method thereof. The high-elasticity silicone rubber lettering film comprises a load-bearing substrate, a release layer, a surface layer silicone rubber ink layer, an anti-photopermeable silicone rubber ink layer, an adhesive interfacial agent layer and a TPU hot melt adhesive film which are connected from bottom to top successively; the adhesive interfacial agent layer is prepared from an adhesive interfacial agent, and the adhesive interfacial agent is an adhesive interfacial agent containing dual-resin components. The silicone rubber lettering film has the advantages of both thesilicone rubber material and the TPU material, has good elasticity, tearing strength, weatherability, yellowing resistance, water washing resistance and other comprehensive properties, has layers noteasy to separate, is not easy to discolor or fade by sunlight exposure, and has the water-washing fading rate not less than 0.5%, is not easy to dye when being mixed and washed with materials with other colors, and is high in stability, easy to bond with a variety of materials, high in bonding strength, not easy to peel off, good in stability, delicate in hand feeling, good in aesthetic degree, and long in service life.