A method for preparing a multi-channel 3D nano texture mold comprises the steps of: coating photoresist on a PET photoetching material, and then entering a stainless steel dust-free furnace for dryingfor standby; arranging a mask pattern plate on the raw rubber surface of the PET photoetching material, vacuuming for photoetching, taking out the mask pattern plate after the photoetching is completed, putting the prepared developer into a cleaning box, putting the photoetching-finished PET photoetching material into the cleaning box for cleaning, displaying the pattern on the PET photoetching material, dehydrating and spinning to obtain a first texture layer; then, performing second texture layer photoetching process which is similar to the first layer, realizing second texture processing superposition to obtain the multi-channel 3D nano-texture mold. The method has the advantages of simple manufacturing process, low cost, reusability, multi-texture superposition, better texture rendering effect and improved working efficiency.