Method for preparing biological carbon immobilized microorganism

A technology of immobilizing microorganisms and biochar, which is applied in the field of environmental engineering, can solve the problems of boric acid toxicity, hindering the mass transfer of beads, etc., and achieve the effect of safe preparation process, high moisture content, and low requirements for conditions and equipment

Inactive Publication Date: 2017-03-22
ZHONGBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The most simple and economical method to prepare immobilized particles with polyvinyl alcohol is the polyvinyl alcohol-boric acid method, but this method has two

Method used

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  • Method for preparing biological carbon immobilized microorganism
  • Method for preparing biological carbon immobilized microorganism

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0033] Example 1

[0034] A preparation method of biochar immobilized microorganisms includes the following steps:

[0035] (1) Weigh 0.8g of biomass charcoal prepared at 300°C, add 20ml of bacterial solution that has been cultured for 22 hours; among them, the strain is obtained from Pseudomonas and Sphingomonas as parents using protoplast fusion technology Fusion strain F14, the inoculum amount is 20%;

[0036] (2) Weigh 8g polyvinyl alcohol and 1g sodium alginate into a 100ml Erlenmeyer flask, add 50ml and 20ml distilled water to soak overnight, shake the sodium alginate solution on a shaker; the next day the polyvinyl alcohol solution shakes in a water bath About 3h to dissolve evenly, add sodium alginate solution at room temperature, and rinse the inner wall with 5ml distilled water;

[0037] (3) Mix the bacteria solution added with biomass charcoal with the solution in step (2) to obtain a mixed bacteria solution, and rinse the inner wall with 5ml of distilled water, at this ti...

Example Embodiment

[0042] Example 2

[0043] A preparation method of biochar immobilized microorganisms includes the following steps:

[0044] (1) Weigh 0.8g of biomass charcoal prepared at 500°C, and add 20ml of bacterial solution that has been cultured for 22 hours;

[0045] (2) Weigh 8g polyvinyl alcohol and 1g sodium alginate into a 100ml Erlenmeyer flask, add 50ml and 20ml distilled water to soak overnight, shake the sodium alginate solution on a shaker; shake the polyvinyl alcohol solution in a water bath for 3h the next day About to dissolve evenly, add sodium alginate solution at room temperature, and rinse the inner wall with 5ml distilled water;

[0046] (3) Mix the bacteria solution added with biomass charcoal with the solution in step (2) to obtain a mixed bacteria solution, and rinse the inner wall with 5ml of distilled water, at this time the concentration of polyvinyl alcohol is 8% and the concentration of sodium alginate is 1%;

[0047] (4) Weigh 5g boric acid in 100ml distilled water, wa...

Example Embodiment

[0051] Example 3

[0052] A preparation method of biochar immobilized microorganisms includes the following steps:

[0053] (1) Weigh 0.8g of biomass charcoal prepared at 700°C and add 20ml of bacteria liquid that has been cultured for 2 hours;

[0054] (2) Weigh 8g polyvinyl alcohol and 1g sodium alginate into a 100ml Erlenmeyer flask, add 50ml and 20ml distilled water to soak overnight, shake the sodium alginate solution on a shaker; shake the polyvinyl alcohol solution in a water bath for 3h the next day About to dissolve evenly, add sodium alginate solution at room temperature, and rinse the inner wall with 5ml distilled water;

[0055] (3) Mix the bacteria solution added with biomass charcoal with the solution in step (2) to obtain a mixed bacteria solution, and rinse the inner wall with 5ml of distilled water, at this time the concentration of polyvinyl alcohol is 8% and the concentration of sodium alginate is 1%;

[0056] (4) Weigh 5g boric acid in 100ml distilled water, wait un...

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Abstract

The invention belongs to the technical field of environmental engineering, and discloses a method for preparing a biological carbon immobilized microorganism. The preparation method comprises the following steps: uniformly mixing a polyvinyl alcohol solution with a sodium alginate solution to form a mixed gel solution; mixing a biological carbon adsorbed microorganism bacterial suspension with the mixed gel solution to obtain a mixed bacterial liquid; dropping into a calcium chloride (CaCl2) solution with saturated boric acid for crosslinking, and transferring into an anhydrous sodium sulfate solution for crosslinking to prepare immobilized beads; and washing with normal saline for 3 times for later use. The prepared immobilized beads have the advantages of large specific surface area, high opening ratio, high moisture content and high microorganism activity.

Description

technical field [0001] The invention belongs to the technical field of environmental engineering, and in particular relates to a method for preparing biochar-immobilized microorganisms. Background technique [0002] Immobilized microorganism technology is a new biotechnology developed on the basis of immobilized enzyme technology. It can greatly increase the concentration of microorganisms, make microorganisms not easy to lose and use for a long time, and their anti-toxicity and tolerance are significantly increased. Solid-liquid separation is easy, less secondary pollution. [0003] The methods of immobilization of microbial cells mainly include adsorption method, carrier binding method, cross-linking method and embedding method. A single immobilization method has its own advantages and disadvantages. The composite immobilization method combining adsorption-embedding-crosslinking can be used in To a certain extent, these problems can be avoided. [0004] Polyvinyl alcohol...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/10C12N11/08C12R1/38C12R1/01
CPCC12N11/14C12N11/08C12N11/10
Inventor 卢静侯彬张松王海芳贾峰刘鹏霄刘侃侃刘晓庆
Owner ZHONGBEI UNIV
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