Preparation method of procyanidin molecular imprinting sensor

A technology of proanthocyanidins and molecular imprinting, applied in instruments, scientific instruments, analytical materials, etc., can solve problems such as poor regeneration and reversibility, high detection limit, slow electron transfer speed and response, and achieve high affinity and selectivity, Response to improved effects

Inactive Publication Date: 2017-08-29
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the thickness of the imprinted film prepared by the traditional imprinting method is difficult to control, and the high cross-linking degree makes the electron transfer speed and response slow, the detection limit is high, and the regeneration and reversibility are poor, which affects the application of molecular imprinting technology in electrochemical sensors.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Preparation of graphene platinum modification solution: In the reactor, add deionized water: 92 mL, chloroplatinic acid: 2 g, graphene oxide: 6 g, ultrasonic at room temperature for 15 minutes, and disperse evenly to obtain graphene nano-platinum Correction fluid;

[0024] (2) Preparation of graphene nano-platinum-modified glassy carbon electrode: Polish the glassy carbon electrode with 0.2 μm and 0.01 μm polishing powder in turn, then ultrasonically clean it with double distilled water, dry it, and drop 23 μL graphite on the surface of the glassy carbon electrode Graphene platinum modification solution, placed under an infrared lamp, and after the solvent is evaporated, the graphene nano-platinum modified glassy carbon electrode is obtained;

[0025] (3) Preparation of proanthocyanidin molecularly imprinted polymer: In the reactor, add deionized water: 60 mL, hydroxyethyl methacrylate: 8 g, methylene succinic acid: 8 g, 1-propenyl-3-butyl Imidazolium hexafluoropho...

Embodiment 2

[0028] (1) Preparation of graphene platinum modification solution: In the reactor, add deionized water: 94 mL, chloroplatinic acid: 1 g, graphene oxide: 5 g, ultrasonic at room temperature for 15 min, and disperse evenly to obtain graphene nano-platinum Correction fluid;

[0029](2) Preparation of graphene nano-platinum-modified glassy carbon electrode: Polish the glassy carbon electrode with 0.2 μm and 0.01 μm polishing powder in turn, then ultrasonically clean it with double distilled water, dry it, and drop 20 μL of graphite on the surface of the glassy carbon electrode Graphene platinum modification solution, placed under an infrared lamp, and after the solvent is evaporated, the graphene nano-platinum modified glassy carbon electrode is obtained;

[0030] (3) Preparation of proanthocyanidin molecularly imprinted polymer: In the reactor, add deionized water: 65mL, hydroxyethyl methacrylate: 6g, methylene succinic acid: 6g, 1-propenyl-3-butyl Imidazolium hexafluorophosphat...

Embodiment 3

[0033] (1) Preparation of graphene platinum modification solution: In the reactor, add deionized water: 90 mL, chloroplatinic acid: 3 g, graphene oxide: 7 g, ultrasonic at room temperature for 15 min, and disperse evenly to obtain graphene nano-platinum Correction fluid;

[0034] (2) Preparation of graphene nano-platinum-modified glassy carbon electrode: Polish the glassy carbon electrode with 0.2 μm and 0.01 μm polishing powder in turn, then ultrasonically clean it with twice distilled water, dry it, and drop 25 μL graphite on the surface of the glassy carbon electrode Graphene platinum modification solution, placed under an infrared lamp, and after the solvent is evaporated, the graphene nano-platinum modified glassy carbon electrode is obtained;

[0035] (3) Preparation of proanthocyanidin molecularly imprinted polymer: In the reactor, add deionized water: 56 mL, hydroxyethyl methacrylate: 10 g, methylene succinic acid: 10 g, 1-propenyl-3-butyl Imidazolium hexafluorophosph...

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Abstract

The invention discloses a preparation method of a procyanidin molecular imprinting sensor. The preparation method comprises modifying a glassy carbon electrode with graphene oxide and chloroplatinic acid, adding 56 to 66% by mass of deionized water, 6 to 10% by mass of hydroxyethyl methacrylate, 6 to 10% by mass of methylene succinic acid, 12 to 20% by mass of 1-allyl-3-butylimidazolium hexafluorophosphate, 1.0 to 4.0% by mass of dimethyl azobisisobutyrate and 0.5 to 2.0% by mass of procyanidin into a reactor, stirring the materials in an oxygen-free atmosphere for a reaction for 6-8h, removing a template molecule through a mixed solution of methanol and acetic acid, carrying out drying to obtain a procyanidin molecular imprinting polymer, and dispensing the procyanidin molecular imprinting polymer to an electrode modified by graphene oxide and chloroplatinic acid to obtain the procyanidin molecular imprinting sensor. The procyanidin molecular imprinting sensor has high affinity and selectivity, has high sensitivity, good specificity and a rapid detection rate and can be recycled.

Description

technical field [0001] The present invention relates to a preparation method of a molecularly imprinted sensor and the technical field of rapid detection application, in particular to a preparation method of a proanthocyanidin molecularly imprinted sensor, which is based on the specific recognition of molecular imprinting and is used to detect drugs, food, biological Proanthocyanidins technology in samples. Background technique [0002] Proanthocyanidins, referred to as OPC, its molecular formula is C 30 h 12 o 6 , with a relative molecular mass of 468.24, is a bioflavonoid with a special molecular structure, and is currently internationally recognized as the most effective natural antioxidant for eliminating free radicals in the human body. It is generally a reddish-brown powder with a slight gas and astringent taste, soluble in water and most organic solvents. The latest research shows that blueberry leaf extract proanthocyanidins can prevent the replication of hepatit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/30
CPCG01N27/308G01N27/48
Inventor 李慧芝赵淑英杨春霞
Owner UNIV OF JINAN
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